Comparative study of class 1 integron, ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline (ACSSuT) and fluoroquinolone resistance in various Salmonella serovars from humans and animals

Hsu, Yu-Ting; Tang, Chiu-Ying; Lin, Hsuan Ming; Chen, Yu‐Hsin; Chen, Yulin; Su, Yu-Heng; Chen, Daniel S.; Lin, Jiunn-Horng; Chang, Chao‐Chin

doi:10.1016/j.cimid.2012.08.004

Cited by 19 publications

(19 citation statements)

References 41 publications

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“…enterica isolates exhibited resistance to chloramphenicol. This finding is supported by other studies that reported the presence of floR gene in various S. enterica as part of Salmonella pathogenicity island-1 23, 32. The resistance to tetracycline is highly associated with the acquisition and expression of efflux pumps, encoded by tet genes, that reduce the concentration of the drug inside the bacterial cells.…”

Section: Discussionsupporting

confidence: 78%

Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia

El-Tayeb

Ibrahim

Al‐Salamah

et al. 2017

Brazilian Journal of Microbiology

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Salmonella is recognized as a common foodborne pathogen, causing major health problems in Saudi Arabia. Herein, we report epidemiology, antimicrobial susceptibility and the genetic basis of resistance among S. enterica strains isolated in Saudi Arabia. Isolation of Salmonella spp. from clinical and environmental samples resulted in isolation of 33 strains identified as S. enterica based on their biochemical characteristics and 16S-rDNA sequences. S. enterica serovar Enteritidis showed highest prevalence (39.4%), followed by S. Paratyphi (21.2%), S. Typhimurium (15.2%), S. Typhi and S. Arizona (12.1%), respectively. Most isolates were resistant to 1st and 2nd generation cephalosporin; and aminoglycosides. Moreover, several S. enterica isolates exhibited resistance to the first-line antibiotics used for Salmonellosis treatment including ampicillin, trimethoprim–sulfamethoxazole and chloramphenicol. In addition, the results revealed the emergence of two S. enterica isolates showing resistance to third-generation cephalosporin. Analysis of resistance determinants in S. enterica strains (n = 33) revealed that the resistance to β-lactam antibiotics, trimethoprim–sulfamethoxazole, chloramphenicol, and tetracycline, was attributed to the presence of carb-like, dfrA1, floR, tetA gene, respectively. On the other hand, fluoroquinolone resistance was related to the presence of mutations in gyrA and parC genes. These findings improve the information about foodborne Salmonella in Saudi Arabia, alarming the emergence of multi-drug resistant S. enterica strains, and provide useful data about the resistance mechanisms.

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Section: Discussionsupporting

confidence: 78%

Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia

El-Tayeb

Ibrahim

Al‐Salamah

et al. 2017

Brazilian Journal of Microbiology

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“…In this study, Sal 4, Sal 7, Sal 8, and Sal 11 isolates produced the well-known ACSSuT Penta-resistance pattern (ampicillin, chloramphenicol, streptomycin, sulfisoxazole, and tetracycline; Hsu et al, 2013). The tetR gene was expressed in Sal 4, Sal 7, Sal 8, and Sal 11 isolates, and tetM, tetS genes were expressed in Sal 6, Sal 7, and Sal 8 with other additional tetracycline resistance genes.…”

Section: Discussionmentioning

confidence: 73%

Alternate antimicrobial resistance genes in multidrug resistant Salmonella spp. isolated from retail meats in Vietnam using RNA‐sequencing analysis

Nghiêm

Nguyen

Jeung

et al. 2019

Journal of Food Safety

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The aim of this study was to examine the resistome characteristics of Salmonella spp. Contamination found in raw retail beef, pork, and chicken samples collected in Hanoi, Vietnam. Approximately 28% (25/90) of samples tested carried Salmonella bacteria. A total of 13/25 (52%) of these Salmonella bacteria were resistant to at least one antibiotic and 9/25 (36%) of the isolates were found to be multidrug resistant. RNA‐seq analysis detected the expression of 107 antibiotic resistance genes (e.g., 22 β‐lactam, 46 aminoglycosides, 8 quinolones, 7 chloramphenicol, 6 tetracycline, and 6 sulfonamide‐trimethoprim resistance genes, and 12 other antimicrobial resistance genes). The parC (S80R and novel mutations A628S) and gyrA (S83F and D87G) mutations contributed to ciprofloxacin resistance in Salmonella Indiana. The findings illustrate the significant potential for multiple‐antibiotic resistant Salmonella in raw retail meats from Hanoi markets, and also provide abundant data to better understand the multidrug resistant Salmonella resistome. Practical Applications Studying the antibiotic resistance characteristics of Salmonella will provide important information for the prevention, control of diseases as well as food contamination control and regulations on the use of antibiotics in treatment and animal husbandry in order to limit antibiotic resistance of bacteria. Studying the expression genome of Salmonella, especially antibiotic resistance genes in multi‐antibiotic resistant Salmonella isolates, provides insight into the molecular epidemiology of antibiotic resistance genes. More importantly, it is possible to detect new mutations in antibiotic resistance genes that cause antibiotic resistance in Salmonella. In addition, studying the expression genome could help to identify new gene groups that can cause antibiotic resistance in this bacterium.

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“…Twenty-eight Salmonella Newport isolates were resistant to at least three antibiotics (Table 1), while 19 (16.4%) presented the now familiar penta-resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides and tetracycline, known as the ACSSuT phenotype, a common characteristic of Salmonella infections and closely linked to Class 1 integrons (Hsu et al, 2013). Mobile genetic elements including integrons, insertion sequences and transposons play a crucial role in facilitating the exchange and spreading of resistance genes between Gram-negative and Gram-positive bacteria (Partridge et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Phenomic and genomic approaches to studying the inhibition of multiresistant Salmonella enterica by microcin J25

Said

Emond-Rhéault

Soltani

et al. 2020

Environmental Microbiology

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Summary In livestock production, antibiotics are used to promote animal growth, control infections and thereby increase profitability. This practice has led to the emergence of multiresistant bacteria such as Salmonella, of which some serovars are disseminated in the environment. The objective of this study is to evaluate microcin J25 as an inhibitor of Salmonella enterica serovars of various origins including human, livestock and food. Among the 116 isolates tested, 37 (31.8%) were found resistant to at least one antibiotic, and 28 were multiresistant with 19 expressing the penta‐resistant phenotype ACSSuT. Microcin J25 inhibited all isolates, with minimal inhibitory concentration values ranging from 0.06 μg/ml (28.4 nM) to 400 μg/ml (189 μM). Interestingly, no cross‐resistance was found between microcin J25 and antibiotics. Multiple sequence alignments of genes encoding for the different proteins involved in the recognition and transport of microcin J25 showed that only ferric‐hydroxamate uptake is an essential determinant for susceptibility of S. enterica to microcin J25. Examination of Salmonella strains exposed to microcin J25 by transmission electronic microscopy showed for the first‐time involvement of a pore formation mechanism. Microcin J25 was a strong inhibitor of several multiresistant isolates of Salmonella and may have a great potential as an alternative to antibiotics.

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Comparative study of class 1 integron, ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline (ACSSuT) and fluoroquinolone resistance in various Salmonella serovars from humans and animals

Cited by 19 publications

References 41 publications

Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia

Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia

Alternate antimicrobial resistance genes in multidrug resistant Salmonella spp. isolated from retail meats in Vietnam using RNA‐sequencing analysis

Phenomic and genomic approaches to studying the inhibition of multiresistant Salmonella enterica by microcin J25

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