Comparative Study of Cis- and Trans-Priming Effect of PEG and BABA in Cowpea Seedlings on Exposure to PEG-Induced Osmotic Stress

Abstract

The growth and performance of cowpea seedlings are negatively impacted by climate change and the subsequent occurrence of drought stress. Osmotic stress leads to the formation of reactive oxygen species, causing membrane breakdown, and impairs metabolic activities. The harmful effects of osmotic stress can be reduced by using seed priming techniques. Seeds of cowpea var. Anaswara were treated with polyethylene glycol (PEG) and β-amino butyric acid (BABA) as priming agents. The seedlings emerged from the primed seeds have been found to reduce the lipid peroxidation rates and improve plant water status by accumulating osmolytes such as proline, total free aminoacids, and total soluble sugars, and also enhanced the production of non-enzymatic antioxidants such as total phenolics, ascorbate, and glutathione, as well as increased the activities of enzymatic antioxidants such as catalase, peroxidase, and superoxide dismutase, which effectively scavenge ROS and maintain the homeostasis of the cell. PEG priming (cis-priming) and BABA priming (trans-priming) exhibited differential physiochemical responses in cowpea subjected to PEG stress. The current work investigates the extent of stress tolerance acquired through seed priming, and it will help to make a sensitive variety to a more tolerant one. Physiochemical responses of seedlings emerged from BABA-primed seeds towards PEG stress were better regulated to encounter the PEG-induced osmotic stress than the seedlings emerged from PEG-primed seeds.

Bolstering Wheat’s Immunity: BABA-Mediated Defense Priming AgainstBipolaris sorokinianaAmid Competition

“…Proline is an osmoprotectant, and its accumulation helps to maintain the integrity of the cell membrane, osmotic balance, and ROS detoxification against oxidative stress (Aswathi et al, 2023). Exogenous application of BABA increased the production of proline in Vicia faba under oxidative stress (Abid et al, 2020).…”

Supplementation of the Plant Conditioner ELICE Vakcina® Product with β-Aminobutyric Acid and Salicylic Acid May Lead to Trans-Priming Signaling in Barley (Hordeum vulgare)

“…To reduce the effect of osmotic stress, cowpea seeds were treated with polyethylene glycol (PEG) and β-aminobutyric acid (BABA) as priming elicitors. The use of PEG as cis -priming and BABA as trans -priming agents showed different physiochemical responses to PEG stress; BABA-primed seeds showed better regulation of osmotic stress [ 7 ].…”

Improving germination and growth performances of millet grown under saline conditions through seed priming

“…En effet, la réussite de la germination des graines est étroitement liée à l'installation de cultures saines et uniformes dans ces conditions difficiles. Les contraintes osmotiques (salinité et sécheresse) affectent la germination, la croissance des plantules et leur vigueur, la phase végétative, la floraison et la fructification à des degrés variables conduisant à des baisses de rendement et de qualité de la production (Sen and Puthur, 2023). Cependant, le stade de germination constitue l'étape clé de développement de la plante et détermine sa tolérance ou sa sensibilité.…”

“…Les méthodes de prétraitement des graines se basent sur plusieurs agents, notamment l'eau (hydro-priming) (Kaya et al 2006), les facteurs d'osmorégulation (osmopriming) (Jisha et al 2013), les macro ou microéléments (nutri-priming) (Alves et al 2019) et les phytohormones (hormo-priming) (Mansour et al 2019;Ellouzi et al 2021). D'autres études ont proposé les Espèces Réactives à l'Oxygène (chimio ou redox-priming) comme agents efficaces dans le processus de prétraitement des graines (Sen and Puthur, 2023). Il convient de signaler que la plupart des recherches sur le priming ont montré des effets bénéfiques de tous les agents utilisés, en favorisant une synchronisation de la germination, une meilleure croissance, une floraison plus précoce et une tolérance accrue aux stress abiotiques tels que la salinité et/ou la sécheresse (Boucelha et al 2021).…”