Comparative Study of Cardioprotective Effect of Various Diameter and Composition Liposomes During Reperfusion of the Isolated Rat Hearts After Normothermic Ischemia

Торопова, Яна Геннадьевна; Мухамадияров, Р. А.; Головкин, А. С.

doi:10.20538/1682-0363-2013-1-55-60

Cited by 1 publication

(3 citation statements)

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“…These results imply that LFI of hearts with liposomes in our experiments induced a vasodilatory effect that was independent of the myocardial needs during reperfusion. Indeed, we have shown previously that recovery of CF in hearts treated with liposomes can be associated with increased activity of the endothelial Nitric Oxide synthase, which brought about vasodilation and endothelial protection by reducing lipid peroxidation 21…”

Section: Discussionmentioning

confidence: 90%

“…The rate of penetration of the liposomes to the infarcted area was slower but they remained in the myocardium for longer. Previously, we have shown the ability of liposomes of ~70 nm loaded with antioxidants to attenuate I/R injury 12,21,22. Interestingly, in that study, even the liposomes without antioxidants induced a cardioprotective effect.…”

Section: Discussionmentioning

confidence: 98%

“…Indeed, we have shown previously that recovery of CF in hearts treated with liposomes can be associated with increased activity of the endothelial nitric oxide synthase, which brought about vasodilation and endothelial protection by reducing lipid peroxidation. 21 …”

Section: Discussionmentioning

confidence: 99%

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Size-Dependent Ability of Liposomes to Accumulate in the Ischemic Myocardium and Protect the Heart

Mukhamadiyarov

Senokosova

Krutitsky

et al. 2018

Journal of Cardiovascular Pharmacology

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Abstract:Liposomes have the potential to be used for drug delivery. Meanwhile, liposome size may affect their accumulation in the target tissue. We investigated the myocardial accumulation of 2 populations of liposomes (∼70 and 110 nm diameter) during ischemia and their effect on ischemia/reperfusion injury. Isolated rat hearts were subjected to 30 minutes of low-flow ischemia with the liposomes, followed by 30 minutes of liposome-free reperfusion. The liposomes were loaded with the fluorescent dye Nile Red to assess their accumulation in the myocardium. The cardiac functional recovery during reperfusion was evaluated using force–velocity characteristics and coronary flow (CF). Reperfusion injury was evaluated by lactate dehydrogenase release. In addition, CF and contractility were assessed in hearts perfused normally with 70 nm liposomes. There was a 6- and 4-fold greater accumulation of the small liposomes in the myocardium and mitochondria, respectively, compared with the large liposomes. Importantly, even without any incorporated drugs, both populations of liposomes improved functional recovery and reduced lactate dehydrogenase release. However, the smaller liposomes showed significantly higher protective and vasodilatory effects during reperfusion than the larger particles. These liposomes also increased CF and contractility during normal perfusion. We suggest that the protective properties of the liposomes could be related to their membrane-stabilizing effect.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 98%