Comparative Study of Blood Cross Matching Using Conventional Tube and Gel Method

Swarup, Davendra; Dhot, PS; Kotwal, Jyoti; Verma, Amita

doi:10.1016/s0377-1237(08)80054-8

Cited by 11 publications

(15 citation statements)

References 6 publications

(5 reference statements)

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“…[22][23][24] In particular, its use in human medicine to identify weak alloantibodies against Rh and other blood groups as well as cross-match incompatibilities (with and without the addition of human antiglobulin [Coombs'] reagent) has improved antibody recognition and titer strength determination when using the gel column compared with the traditional tube. [25][26][27] In people, gel-typing uses mostly monoclonal reagents and is commercially available for various blood types. Similarly, this gel column technology has been recently introduced for DEA 1.1 and feline AB typing using monoclonal antibodies, 15,17,19 and in the present study its application for extended typing for DEA 3, 4, 7, and Dal as well as cross-matching was investigated and found to be useful.…”

Section: Discussionmentioning

confidence: 99%

ORIGINAL RESEARCH: Dog erythrocyte antigens 1.1, 1.2, 3, 4, 7, and Dal blood typing and cross-matching by gel column technique

Kessler

Reese

Chang

et al. 2010

Veterinary Clinical Pathology

173

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Background Testing for canine blood types other than dog erythrocyte antigen 1.1 (DEA 1.1) is controversial and complicated by reagent availability and methodology. Objectives The objectives of this study were to use available gel column technology to develop an extended blood-typing method using polyclonal reagents for DEA 1.1, 1.2, 3, 4, 7, and Dal and to assess the use of gel columns for cross-matching. Methods Dogs (43–75) were typed for DEA 1.1, 1.2, 3, 4, 7, and Dal. Methods included tube agglutination (Tube) using polyclonal reagents, a commercially available DEA 1.1 gel column test kit (Standard-Gel) using monoclonal reagent, and multiple gel columns (Extended-Gel) using polyclonal reagents. Blood from 10 recipient and 15 donor dogs was typed as described above and cross-matched using the gel column technique. Results Of 43 dogs typed for DEA 1.1, 23, 25, and 20 dogs were positive using Standard-Gel, Extended-Gel, and Tube, respectively. Typing for DEA 1.2 was not achievable with Extended-Gel. For 75 dogs typed for DEA 3, 4, and 7, concordance of Extended-Gel with Tube was 94.7%, 100%, and 84%, respectively. Dal, determined only by Extended-Gel, was positive for all dogs. Post-transfusion major cross-matches were incompatible in 10 of 14 pairings, but none were associated with demonstrable blood type incompatibilities. Conclusions Gel column methodology can be adapted for use with polyclonal reagents for detecting DEA 1.1, 3, 4, 7, and Dal. Agglutination reactions are similar between Extended-Gel and Tube, but are more easily interpreted with Extended-Gel. When using gel columns for cross-matching, incompatible blood cross-matches can be detected following sensitization by transfusion, although in this study incompatibilities associated with any tested DEA or Dal antigens were not found.

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Section: Discussionmentioning

confidence: 99%

ORIGINAL RESEARCH: Dog erythrocyte antigens 1.1, 1.2, 3, 4, 7, and Dal blood typing and cross-matching by gel column technique

Kessler

Reese

Chang

et al. 2010

Veterinary Clinical Pathology

173

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“…Thus, compatibility testing with gel method can be considered as a method with simple, fast and stable stages so that it can be documented and provide objective results. [3][4][5] Procedure of the compatibility testing is divided into two stages, namely major compatibility examination in which the donor's erythrocytes are mixed with the patient's serum and minor compatibility examination in which the patient's erythrocytes are mixed with the donor's plasma. 4 The International Society of Blood Transfusion (ISBT) has introduced 285 blood group antigens.…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5] Procedure of the compatibility testing is divided into two stages, namely major compatibility examination in which the donor's erythrocytes are mixed with the patient's serum and minor compatibility examination in which the patient's erythrocytes are mixed with the donor's plasma. 4 The International Society of Blood Transfusion (ISBT) has introduced 285 blood group antigens. ABO blood group system and rhesus blood group are the most clinically significant group system.…”

Section: Introductionmentioning

confidence: 99%

CHARACTERISTICS OF CROSSMATCH TYPES IN COMPATIBILITY TESTING ON DIAGNOSIS AND BLOOD TYPES USING GEL METHOD (Ciri Inkompatibilitas Uji Cocok Serasi Metode Gel terhadap Diagnosis dan Golongan Darah)

Irawaty¹,

Am²,

Arif³

2018

Indonesian J. Clin. Pathol. Med. Lab.

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Pemeriksaan uji cocok serasi adalah pemeriksaan kesesuaian darah pasien dan donor. Pemeriksaan ini untuk mengetahui apakahantigen eritrosit donor sesuai dengan antibodi di serum pasien (uji mayor) dan antigen eritrosit pasien terhadap antibodi di serum donor(uji minor). Pemeriksaan uji cocok serasi dapat dilakukan dengan metode tabung (metode konvensional) dan Gel. Penelitian ini bertujuanuntuk mengetahui ciri golongan darah dan diagnosis di inkompatibilitas uji cocok serasi dengan menggunakan metode Gel. Rancanganpenelitian berupa observasional retrospektif di Bank Darah Rumah Sakit (BDRS) RSUP Dr.Wahidin Sudirohusodo Makassar denganmengambil data Sistem Informasi Rumah Sakit (SIRS) dan data rekam medis masa waktu Juni 2013-Juni 2014. Jumlah keseluruhan 213sampel dengan jumlah laki-laki 105 orang (49,3%) dan perempuan 108 orang (50,7%). Rentang umur terbanyak >17 tahun (83,6%).Kelompok di inkompatibilitas terbanyak di penyakit infeksi (36,2%) dengan golongan darah B (32,9%), rhesus positif (100%) dan tipeinkompatibilitas minor (81,2%). Pasien tanpa riwayat transfusi lebih banyak (97,7%) dibandingkan dengan riwayat transfusi (2,3%).Perlu diteliti lebih lanjut dengan anamnesis langsung di pasien untuk menghindari bias sehingga diperoleh hasil yang lebih sahih. Selainitu diperlukan penelitian lebih lanjut pemeriksaan penyaringan dan identifikasi antibodi di setiap ketidaksesuaian uji cocok serasi.

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“…Hal tersebut hasilnya sangat subyektif (bergantung keahlian petugas bank darah), diperlukan pencucian sel tiga kali serta hasil reaksi secara kasat mata tidak dapat di didokumentasikan. [5][6][7] Pemeriksaan banding silang dengan metode gel merupakan kegiatan dengan tahapan yang terbakukan, sederhana, cepat, serta memberikan hasil yang sesuai tujuan. Metode gel, pertama kali ditemukan oleh Lapierre 1 pada tahun 1990 di Regional Blood Transfusion Center of Lyon.…”

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TURNAROUND TIME UJI COCOK SERASI DI PELAYANAN BANK DARAH (Turnaround Time Cross Match in the Blood Bank)

Nurtanio¹,

Muhiddin²,

Arif³

2018

Indonesian J. Clin. Pathol. Med. Lab.

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Turnaround Time (TAT) is the time between the blood request form arriving at the blood bank and when it is issued by the bloodbank. Blood Bank RSWS standard on the TAT of Stat blood service is <45 minutes and is regularly <60 minutes. The aim of this researchwas to know the TAT of blood services at the Blood Bank of the Dr Wahidin Sudirohusodo Hospital, Makassar. The research was carriedout by cross sectional study between August until November 2013. The data were divided into two (2) categories, which were Stat andregular blood service. The blood service was divided into three (3) service times, morning (08:00–14:00), afternoon (14:00–21:00) andevening (21:00–08:00). The statistical method used was Independent Samples T Test. There were 1.366 blood services consisting of 831Stat blood service with average TAT about 37.15 minutes (82.9% complied to standard) and 535 regular blood service with averageTAT about 45.73 minutes (96.1% complied to standard). There were significant differences between morning and afternoon (p=0.000)and between afternoon and evening Stat TAT (p=0.003), but there was no significant difference between morning and evening StatTAT (p=0.196). No significant difference was found in regular TAT between the morning and afternoon session (p=0.915), as well asnoon and evening (p=0.490); and morning and evening session (p=0.428). The TAT blood service at the Dr. Wahidin SudirohusodoHospital Blood Bank which was carried out by gel method should shorten the waiting time of Stat and regular blood service, but not upto 100% yet. Based on this study, the researchers recommended to do some regulation changes in the blood service system especiallyfor the morning and evening Stat sessions.

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Comparative Study of Blood Cross Matching Using Conventional Tube and Gel Method

Cited by 11 publications

References 6 publications

ORIGINAL RESEARCH: Dog erythrocyte antigens 1.1, 1.2, 3, 4, 7, and Dal blood typing and cross-matching by gel column technique

ORIGINAL RESEARCH: Dog erythrocyte antigens 1.1, 1.2, 3, 4, 7, and Dal blood typing and cross-matching by gel column technique

CHARACTERISTICS OF CROSSMATCH TYPES IN COMPATIBILITY TESTING ON DIAGNOSIS AND BLOOD TYPES USING GEL METHOD (Ciri Inkompatibilitas Uji Cocok Serasi Metode Gel terhadap Diagnosis dan Golongan Darah)

TURNAROUND TIME UJI COCOK SERASI DI PELAYANAN BANK DARAH (Turnaround Time Cross Match in the Blood Bank)

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