Comparative Study of Bacterial Identification by Colorectal Tissue Culture and Fluid Culture After Colonoscopic Washing

Abstract

Background: Inflammation of the colon is often found during colonoscopy. In order to confirm the pathogenesis of the lesion, bacterial cultures are applied. Biopsied tissue specimens are used for bacterial culture, and fluid culture after colonoscopic washing also has been used for bacterial identification. In the latter method, sterile saline solution is sprayed over the area and then is collected with the fluid. The two methods have not been compared with each other. Methods: The subjects comprised 89 cases with inflammatory lesions detected during colonoscopy. Samples were collected by both methods at the inflammatory site. The bacterial load was semiquantitatively evaluated as 1+, 2+, and 3+. Results: Pathological bacteria were found in nine cases. Vibrio parahaemolyticus was detected in one case only by tissue culture. In the other eight cases, bacteria cultures were positive by both methods. A total of 36 kinds of bacteria constituting the intestinal flora were identified. The detection rate was 83.9% by fluid culture and 70.9% by tissue culture. It was significantly larger by fluid culture than by tissue culture. Bacteria were isolated at a rate of 3.61 species per sample by fluid culture and 3.05 species per sample by tissue culture. The bacterial load per species was a median of 2.00+ by fluid culture and 1.27+ by tissue culture. The number of detected bacterial species and the bacterial load per species were significantly larger by fluid culture than by tissue culture. Conclusion: Fluid culture after colonoscopic washing is recommended to identify intestinal bacteria by culture.

Combined Endoscopy, Aspiration, and Biopsy Analysis for Identifying Infectious Colitis in Patients With Ileocecal Ulcers

“…We demonstrated that endoscopy is more effective than standard stool collection, especially for diagnosing C jejuni and TB. Suda et al 19 reported a bacterial pathogen detection rate of 83.9% by intestinal fluid culture and 70.9% by tissue culture. Matsumoto et al 18 reported that biopsy specimens were more sensitive than fecal samples to culture (50% vs 20%).…”

“…14,15 Endoscopy is acceptable when GI symptoms persist or worsen, 16 because it enables not only direct visualization but also sample collection during aspiration of intestinal fluid or biopsy. [17][18][19] Having a knowledge of specific visual endoscopic findings of colonic disease may prompt appropriate sample collection methods (biopsy or aspiration) or the use of suitable pathogen detection methods (microscopy, histology, culture, and polymerase chain reaction [PCR]), which can provide clues toward a definite diagnosis. However, few studies have reported on the gross appearance of infectious colitis, [5][6][7][8]12,13 and no objective criteria that are based on endoscopic findings exist.…”

MYCOBACTERIUM LENTIFLAVUM ILEITIS USING ASPIRATED INTESTINAL FLUID DURING ENDOSCOPY IN HIV‐INFECTED PATIENT

“…Endoscopy is not only useful for obtaining an image, but it is also useful for biopsy and aspiration of intestinal fluid 5 . We believe that endoscopic examination should be carried out even if the standard stool culture tests negative, especially in HIV patients who have symptoms of diarrhea.…”