Comparative Study between Serological and Molecular Methods for Diagnosis Bovine Viral Diarrhea Virus

Abstract

The purpose of this study was to determine the specificity and sensitivity of modified serological and molecular tools for the detection of Bovine Viral Diarrhea Virus (BVDV). The study was evaluated using 100 samples of known status blood samples (50 positive and 50 negative), blood sera samples were tested to detect antigens of (BVDV) by using (IDEXX, Herd Check BVDV Ag/Serum Plus) ELISA test and real-time PCR assay were modified to detect the RNA of BVDV in fresh blood samples.The presences of antigens of BVDV in sera were detected in all positive samples by ELISA test, and RT-PCR assay could detect RNA in all positive samples. In conclusion, these serological and molecular tools have 100% specificity and sensitivity for diagnosis BVDV.