Comparative study between routine and molecular diagnosis of Mycobacterium species isolated from humans and bovines

Al-Thwani, Amina N.; Al-Saqur, Ihsan M.; AL-Attar, I.M.

doi:10.1016/j.ijmyco.2014.10.045

Cited by 3 publications

(2 citation statements)

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“…Zoonotic tuberculosis (TB) merupakan penyakit yang menyebar luas, menular, dan terus menjadi penyebab utama kematian. TB merupakan masalah kesehatan masyarakat yang dominan di dunia (Al-Thwani et al, 2015). Mycobacterium tuberculosis adalah penyebab paling umum dari TB manusia dan M. bovis adalah agen penyebab penyakit TB pada sapi.…”

Section: Pendahuluanunclassified

Skrining Susu terhadap Mycobacterium bovis pada Peternakan Sapi Perah di Wilayah Tengah dan Timur Pulau Jawa, Indonesia

Putra,

Basri,

Sudarnika

et al. 2023

Jurnal Sain Veteriner

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Tuberkulosis adalah penyakit dengan tingkat kejadian kasus tinggi pada masyarakat di Indonesia. Bovine tuberculosis pada sapi perah disebabkan oleh bakteri M. bovis, diduga turut berperan dalam menyebabkan kasus tuberkulosis karena dapat menular ke manusia (zoonosis) melalui konsumsi susu. Wilayah tengah dan timur Pulau Jawa merupakan sentra peternakan sapi perah di Indonesia yang masih melaporkan tingginya kasus tuberkulosis pada manusia. Informasi mengenai keberadaan bakteri M. bovis pada susu yang dihasilkan di wilayah tersebut belum pernah dilaporkan. Penelitian ini bertujuan untuk skrining keberadaan M. bovis di susu pada peternakan sapi perah di wilayah tengah dan timur Pulau Jawa. Sampel susu diambil dari 163 ekor sapi menggunakan metode cluster random sampling dari 3 provinsi yaitu Jawa Tengah, Yogyakarta dan Jawa Timur. Sampel susu diuji dengan menggunakan PCR konvensional dengan metode multiplex PCR untuk mengidentifikasi M. bovis dan M. tuberculosis dalam waktu bersamaan. Skrining dengan uji PCR terhadap 163 sampel susu dari peternakan sapi perah di wilayah tengah dan timur Pulau Jawa menunjukkan bahwa susu yang dihasilkan oleh sapi perah yang dipelihara di wilayah ini tidak mengandung bakteri M. Bovis dan M. Tuberculosis.

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Section: Pendahuluanunclassified

Skrining Susu terhadap Mycobacterium bovis pada Peternakan Sapi Perah di Wilayah Tengah dan Timur Pulau Jawa, Indonesia

Putra,

Basri,

Sudarnika

et al. 2023

Jurnal Sain Veteriner

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“…Dehydration can cause the skin to turn bluish. and even death if treatment is delayed [5][6][7][8]. Cholera in humans has been connected to birds after close contact with avian species, which may increase the risk of infections.…”

Section: Introductionmentioning

confidence: 99%

Comparison Between Conventional and Molecular Identification of Vibrio Cholerae Isolated From Human and Birds

Abbas,

Yassein

2025

Egyptian Journal of Veterinary Sciences

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T HIS Study aimed to comparison between diagnostic methods to evaluated the prevalence of Vibrio cholerae .A total of 90 samples include 40 birds faeces were collected from bird farms and birds breeding stores for Ducks, chickens, Pigeons, Pet birds and 50 hand swab from owners of birds (Males and Females) .collection from different places Al-hurya city, Abu Ghraib city, AL-Yarmouk area ,University district area in Baghdad Province, at the period of February 2021 to February 2022, in order to evaluate the routine laboratory diagnostic procedures in the diagnosis of V. cholerae isolates and compared them with molecular methods as Polymerase chain reaction (PCR).V.cholerae has been isolated and identified by using culturing method in addition to biochemical tests, Vitek2 system. The results showed that in culturing method percentage of isolation (43.33%)from birds feces, and (33.33%) in hand swabs,in biochemical tests(33.33%) in birds feces, and (10%)from hand swabs in Vitek2 system (6.7%)from each birds feces and hand swabs. PCR technique was used to detect sodB Gene encoding to outer membrane protein of V. cholerae ,on the PCR results, the rate of V. cholerae isolation from birds feces samples was 23.3 %. and 6.66% from hand swab samples,in Comparison PCR results showed that there were high sensitivity 100% with cultural, biochemical test , while 44% with Vitek2 system respectively.in presnt study found that diagnosis of V.cholerae by routine diagnostic may be misidentifications and using of PCR more faster, accurate ,sensitive and specific for correct identification

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Prevalence of Mycobacterium bovis in milk on dairy cattle farms: An international systematic literature review and meta-analysis

et al. 2022

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Comparative study between routine and molecular diagnosis of Mycobacterium species isolated from humans and bovines

Cited by 3 publications

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Skrining Susu terhadap Mycobacterium bovis pada Peternakan Sapi Perah di Wilayah Tengah dan Timur Pulau Jawa, Indonesia

Skrining Susu terhadap Mycobacterium bovis pada Peternakan Sapi Perah di Wilayah Tengah dan Timur Pulau Jawa, Indonesia

Comparison Between Conventional and Molecular Identification of Vibrio Cholerae Isolated From Human and Birds

Prevalence of Mycobacterium bovis in milk on dairy cattle farms: An international systematic literature review and meta-analysis

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