Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats

Almeida, Otavio Machado de; Jorgetti, Wanda; Oksman, Denis; Jorgetti, Camilo; Rocha, Dalva Marreiro; Gemperli, Rolf

doi:10.1590/s0102-86502013000100011

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…Furthermore, as gamma irradiation is widely used within the food (i.e., at low doses, usually < 5 kGy) and healthcare (i.e., for sterilization, usually at doses > 25 kGy) industries, many reports are available on the influence of the sample processing conditions, sample matrix, and sample conditioning on various sample parameters [44][45][46][47][48]. Specifically, extensive comparative studies and characterization experiments are usually performed when developing novel therapeutic products requiring sterility, as the retained method may drastically alter key and critical product attributes [49][50][51][52]. It is, therefore, highly advisable to consider sterility requirements and validation steps early on in the prototype development phase for sparing use of resources and augmentation of the technical chances of successful development.…”

Section: Effects Of Gamma Irradiation On the Proteome Of Stabilized P...mentioning

confidence: 99%

Lyophilized Progenitor Tenocyte Extracts: Sterilizable Cytotherapeutic Derivatives with Antioxidant Properties and Hyaluronan Hydrogel Functionalization Effects

Laurent

Porcello

Jeannerat³

et al. 2023

Antioxidants

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Cultured primary progenitor tenocytes in lyophilized form were previously shown to possess intrinsic antioxidant properties and hyaluronan-based hydrogel viscosity-modulating effects in vitro. The aim of this study was to prepare and functionally characterize several stabilized (lyophilized) cell-free progenitor tenocyte extracts for inclusion in cytotherapy-inspired complex injectable preparations. Fractionation and sterilization methods were included in specific biotechnological manufacturing workflows of such extracts. Comparative and functional-oriented characterizations of the various extracts were performed using several orthogonal descriptive, colorimetric, rheological, mechanical, and proteomic readouts. Specifically, an optimal sugar-based (saccharose/dextran) excipient formula was retained to produce sterilizable cytotherapeutic derivatives with appropriate functions. It was shown that extracts containing soluble cell-derived fractions possessed conserved and significant antioxidant properties (TEAC) compared to the freshly harvested cellular starting materials. Progenitor tenocyte extracts submitted to sub-micron filtration (0.22 µm) and 60Co gamma irradiation terminal sterilization (5–50 kGy) were shown to retain significant antioxidant properties and hyaluronan-based hydrogel viscosity modulating effects. Hydrogel combination products displayed important efficacy-related characteristics (friction modulation, tendon bioadhesivity) with significant (p < 0.05) protective effects of the cellular extracts in oxidative environments. Overall, the present study sets forth robust control methodologies (antioxidant assays, H2O2-challenged rheological setups) for stabilized cell-free progenitor tenocyte extracts. Importantly, it was shown that highly sensitive phases of cytotherapeutic derivative manufacturing process development (purification, terminal sterilization) allowed for the conservation of critical biological extract attributes.

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Section: Effects Of Gamma Irradiation On the Proteome Of Stabilized P...mentioning

confidence: 99%

Lyophilized Progenitor Tenocyte Extracts: Sterilizable Cytotherapeutic Derivatives with Antioxidant Properties and Hyaluronan Hydrogel Functionalization Effects

Laurent

Porcello

Jeannerat³

et al. 2023

Antioxidants

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“…All the sponges presented a similar elliptical shape and a smooth, soft and sponge-like morphology along with thickness of 2 mm and wide length of ~7 mm. These uniform elliptical shapes can be ascribed to the 3 h refrigeration, freeze-drying and lyophilization [18]. Figure 2 showed the (a) FTIR spectra, (b) compressive strength, and (c) porosity of bare DC/HAp and 1, 5 and 10 μM EGCG/DC/HAp sponges.…”

Section: Discussionmentioning

confidence: 98%

Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration

Kook

Tian

Jeon

et al. 2017

Journal of Biomaterials Science, Polymer Edition

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Scaffolds mimicking structural and chemical characteristics of the native bone tissues are critical for bone tissue engineering. Herein, we have developed and characterized epigallocatechin gallate/duck's feet collagen/hydroxyapatite (EGCG/DC/HAp) composite sponges that enhanced the bone tissue regeneration. The three-dimensional composite sponges were synthesized by loading various amounts (i.e. 1, 5 and 10 μM) of EGCG to duck feet derived collagen followed by freeze-drying and then coating with hydroxyapatite. Several measuremental techniques were employed to examine the properties of the as-fabricated composite sponges including morphology and structure, porosity, compressive strength, etc. and as well compared with pristine duck feet derived collagen. SEM observations of EGCG/DC/HAp sponges showed the formation of a highly porous collagen matrix with EGCG embodiment. The porosity and pore size of sponges were found to increase by high EGCG content. The compressive strength was calculated as 3.54 ± 0.04, 3.63 ± 0.03, 3.89 ± 0.05, 4.047 ± 0.05 MPa for 1, 5 and 10 μM EGCG/DC/HAp sponges, respectively. Osteoblast-like cell (BMSCs isolated from rabbit) culture and in vivo experiments with EGCG/DC/HAp sponges implanted in nude mouse followed by histological staining showed enhanced cell internalization and attachment, cell proliferation, alkaline phosphatase expressions, indicating that EGCG/DC/HAp sponges have ahigh biocompatibility. Moreover, highEGCG content in the EGCG/DC/HAp sponges have led to increased cellular behavior. Collectively, the 5 μM of EGCG/DC/HAp sponges were suggested as the potential candidates for bone tissue regeneration.

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“…Mat-Salleh et al used discarded bone fragments from hip arthroplasty as samples and found that 0.5% chlorhexidine was significantly superior to povidone-iodine and alcohol in disinfecting contaminated bone ( 20 ). Some researchers analyzed the histomorphology of rat allografts after sterilization with high-pressure sterilization, gamma irradiation, and ethylene oxide and found no significant differences among the three groups ( 21 ). Mortazavi et al ( 22 ) conducted a systematic review of the literature on contamination related to bone transplantation and found that the contamination rate of dislodged bone tissue during surgery was close to 40%.…”

Section: Discussionmentioning

confidence: 99%

Successful reimplantation of extruded bone segment in lower limb open fractures: case report and literature review

Hu,

Tan,

Zhu

et al. 2024

Front. Pediatr.

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ObjectiveThe aim of this study is to summarize and demonstrate the different sterilization methods and surgical techniques for open fractures with impacted bone segments in the lower limbs.MethodsA retrospective analysis was conducted on the clinical characteristics, treatment methods, and outcomes of a case involving a 10.5 cm extruded segment of the femur in a 9-year-old male with a right femoral comminuted fracture treated at our center. Additionally, a retrospective review and summary were conducted on all reported cases of open fractures with impacted bone segments in the lower limbs.ResultsOur center treated a 9-year and 11-month-old male child who presented with a Gustilo type IIIB open fracture of the femur along with a large segment of the femur being ejected as a result of a car accident. The child was resuscitated to correct hypovolemic shock, underwent emergency wound debridement, and had Ilizarov external fixation of the femur. The ejected femur segment was sterilized using ethylene oxide and re-implanted four days after the injury. A literature review showed that out of the cases of open fractures with impacted bone segments in the lower limbs, there were 14 cases involving the femur and 5 cases involving the tibia. Among them, sterilization was performed using povidone-iodine in 6 cases, high-pressure steam sterilization in 3 cases, and other methods including gamma-ray irradiation and soaking in antibacterial solution were used in the remaining cases. In terms of surgical methods, 7 cases were fixed with locking plates, 3 cases were fixed with external fixation devices, 1 case was immobilized in a cast, 1 case was fixed with an intramedullary rod, and 4 cases involved a combination of external fixation and internal fixation. The average time for re-implantation was 7.6 days after the injury. There were no serious complications such as infection or non-union observed in any of the cases during follow-up.ConclusionEthylene oxide can be considered a reliable choice for the reimplantation of displaced bone segments in open fractures after sterilization.

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Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats

Cited by 6 publications

References 11 publications

Lyophilized Progenitor Tenocyte Extracts: Sterilizable Cytotherapeutic Derivatives with Antioxidant Properties and Hyaluronan Hydrogel Functionalization Effects

Lyophilized Progenitor Tenocyte Extracts: Sterilizable Cytotherapeutic Derivatives with Antioxidant Properties and Hyaluronan Hydrogel Functionalization Effects

Nature-derived epigallocatechin gallate/duck’s feet collagen/hydroxyapatite composite sponges for enhanced bone tissue regeneration

Successful reimplantation of extruded bone segment in lower limb open fractures: case report and literature review

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