Comparative study among three different methods of bone marrow mesenchymal stem cell transplantation following cerebral infarction in rats

Abstract

The objective of this study was to investigate the effects of transplanted bone marrow mesenchymal stem cells (BMSCs) administered via internal jugular vein injection, carotid artery injection, or intraventricular transplantation for the treatment of cerebral infarction, which was modeled in rats. The neurological scores of the treated rats and the distribution of the transplanted cells (GFP-labeled) in the infarction area were evaluated. The cerebral infarction model was produced by inserting a modified Zea-longa suture, which generated middle cerebral artery occlusion (MCAO). The GFP-labeled BMSCs were transplanted through the jugular vein or the carotid artery or by stereotactic intraventricular delivery to the infarction models 1 week after the cerebral infarction was established. The 'Nerve Function Score' of the model rats was recorded before and after BMSC transplantation. Brain tissue sections were examined under a fluorescence microscope. We determined that the transplanted BMSCs rescued brain function, which was indicated by a decrease in the neurological scores (P<0·05) following BMSC transplantation. The effect of BMSC transplantation was reflected in decreases in the neurological score in the intraventricular transplantation group, the carotid artery transplantation group, and the jugular vein graft group*. The transplanted BMSCs were able to migrate to the brain injury area and the cortex and survived the infarction; thus, BMSCs may promote the recovery of nerve function.

Intracarotid Infusion of Mesenchymal Stem Cells in an Animal Model of Parkinson's Disease, Focusing on Cell Distribution and Neuroprotective and Behavioral Effects

“…This may suggest that the tendency of rMSCs to concentrate in the lesioned hemisphere was more likely due to the mannitol-induced transient BBB opening in this side rather than to a chemotactic attraction exerted by lesion. The distribution pattern was similar to that observed in animal models of stroke following local or systemic administration of MSCs [68,69] and reflects the migratory ability of these cells. The presence of rMSC clusters along the corpus callosum-a preferential migration In the absence of mannitol treatment, no remarkable rMSC signal is detected into the brain of 6-OHDA animals.…”

Brain repair: cell therapy in stroke

“…In the human neuronal MSC group, better cell survival and functional recovery were observed despite the absence of synaptic connection between the transplanted and recipient cerebral cells on fluorescence-in-situ-hybridization (FISH), suggesting that the neuronal differentiation did not contribute to the MSC beneficial effects 53. In experiments with MSCs derived from donor rats,54 mice,55 rabbit,56 (autologous or allogeneic), or humans57 (xenogeneic), cells have been transplanted by IV,57 IA,58 ST,59 or intracisternal58 routes into animals, from hours57 to 1 month60 after induction of stroke with either temporary or permanent MCAo. Homing of the transplanted MSCs appears to occur via a complex multistep process that includes interactions with the stromal cell-derived factor 1 (SDF-1) (also called C-X-C motif chemokine 12 [CXCL12]) chemokine receptor 61.…”

RETRACTED ARTICLE: Effects of BDNF-Transfected BMSCs on Neural Functional Recovery and Synaptophysin Expression in Rats with Cerebral Infarction

“…Besides, BMSCs can survive in infarct brain, migrate towards cerebral infarct, and express neural phenotypes when transplanted into animal with various neurological disorders, such as middle cerebral artery occlusion [7]. For instance, the BMSCs significantly enhance the motor function recovery when transplanted into the animal models with CI, spinal cord injury, and traumatic brain injury [8,9].…”