Comparative Studies to Evaluate Relative in vitro Potency of Luteolin in Inducing Cell Cycle Arrest and Apoptosis in HaCaT and A375 Cells

George, V. Cijo; Ragupathi, Naveen Kumar Devanga; Suresh, P.K.; Kumar, Sanjay; Kumar, R. Ashok

doi:10.7314/apjcp.2013.14.2.631

Cited by 51 publications

(44 citation statements)

References 42 publications

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“…In this study, it was found that luteolin has a remarkable effect in the induction of CCA cells death with the IC 50 value in the low micromolar range. The ability of luteolin to inhibit cell growth in CCA seemed to be much more potent when compared to the effects on other types of cancers as reported by other investigators (Wang et al, 2012;George et al, 2013). To examine the mechanism responsible for cell growth inhibition, cell cycle distribution was evaluated using flow cytometry.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, there are several reports about the anticancer properties of luteolin including induction of cancer cell apoptosis and cell cycle arrest (Wang et al, 2012), and inhibition of cancer cell invasiveness (Ruan et al, 2012) and angiogenesis (Pratheeshkumar et al, 2012). Despite several studies elucidating significant anticancer activity of luteolin against various cancers (Wang et al, 2012;George et al, 2013), its activity against CCA remains to be addressed. The purpose of this study was to investigate the anticancer effect of luteolin on human CCA cells and to gain insights regarding the underlying mechanism mediating its effects.…”

Section: Introductionmentioning

confidence: 99%

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Luteolin Arrests Cell Cycling, Induces Apoptosis and Inhibits the JAK/STAT3 Pathway in Human Cholangiocarcinoma Cells

Aneknan

Kukongviriyapan²,

Prawan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Luteolin Arrests Cell Cycling, Induces Apoptosis and Inhibits the JAK/STAT3 Pathway in Human Cholangiocarcinoma Cells

Aneknan

Kukongviriyapan²,

Prawan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Tang et al (2011) reported luteolin is a unique molecule inhibitor of Nrf2 that can sensitize NSCLC cells to anticancer drugs. And also luteolin indicated a potential role in inducing Cell Cycle Arrest and Apoptosisin A375 human melanoma cells (George et al, 2013). But the low solubility and poor penetration across the cells is the biggest obstacle, which restricts the application of luteolin in therapeutic protocols (Bhattacharya and Ghosh, 2009).…”

Section: Discussionmentioning

confidence: 99%

Luteolin-loaded Phytosomes Sensitize Human Breast Carcinoma MDA-MB 231 Cells to Doxorubicin by Suppressing Nrf2 Mediated Signalling

Sabzichi

Hamishehkar²,

Ramezani³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Nuclear factor erythroid 2-related factor 2 (Nrf2) has been recognized as a transcription factor that controls mechanisms of cellular defense response by regulation of three classes of genes, including endogenous antioxidants, phase II detoxifying enzymes and transporters. Previous studies have revealed roles of Nrf2 in resistance to chemotherapeutic agents and high level expression of Nrf2 has been found in many types of cancer. At physiological concentrations, luteolin as a flavonoid compound can inhibit Nrf2 and sensitize cancer cells to chemotherapeutic agents. We reported luteolin loaded in phytosomes as an advanced nanoparticle carrier sensitized MDA-MB 231 cells to doxorubicin. In this study, we prepared nano phytosomes of luteolin to enhance the bioavailability of luteolin and improve passive targeting in breast cancer cells. Our results showed that cotreatment of cells with nano particles containing luteolin and doxorubicin resulted in the highest percentage cell death in MDA-MB 231cells (p<0.05). Furthermore, luteolin-loaded nanoparticles reduced Nrf2 gene expression at the mRNA level in cells to a greater extent than luteolin alone (p<0.05). Similarly, expression of downstream genes for Nrf2 including Ho1 and MDR1 were reduced significantly (p<0.05). Inhibition of Nrf-2 expression caused a marked increase in cancer cell death (p<0.05). Taken together, these results suggest that phytosome technology can improve the efficacy of chemotherapy by overcoming resistance and enhancing permeability of cancer cells to chemical agents and may thus be considered as a potential delivery system to improve therapeutic protocols for cancer patients.

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“…The keratinocyte colonies are subject to auto-regulation and can be recreated in 2D cell cultures [24,25]. The immortalized HaCat cells still maintain the main characteristics of keratinocytes [26][27][28][29]. The capability of HaCat cells to colonise and proliferate was heavily depended on initial seeding densities.…”

Section: Discussionmentioning

confidence: 99%

Mechanistic Insights of Cells in Porous Scaffolds via Integrated Culture Technologies

Gabbott¹,

Sun²

2017

JLS

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This research aimed to combine 3 cell and tissue culture technologies to obtain mechanistic insights of cells in porous scaffolds. When cultivated on 2D (2-dimensional) surfaces, HDFs (human dermal fibroblasts) behaved individually and had no strict requirement on seeding density for proliferation; while HaCat cells relied heavily on initial densities for proliferation and colony formation, which was facilitated when co-cultured with HDFs. Experiments using a 3D CCIS (3-dimensional cell culture and imaging system) indicated that HDFs colonised open pores of varying sizes (125-420 μm) on modular substrates via bridge structures; while HaCat cells formed aperture structures and only colonised small pores (125 μm). When co-cultured, HDFs not only facilitated HaCat attachment on the substrates, but also coordinated with HaCat cells to colonise open pores of varying sizes via bridge and aperture structures. Based on these observations, a 2-stage strategy for the culture of HDFs and HaCat cells on porous scaffolds was proposed and applied successfully on a cellulosic scaffold. This research demonstrated that cell colonisation in scaffolds was dependent on multiple factors; while the integrated 2D&3D culture technologies and the 3D CCIS was an effective and efficient approach to obtain mechanistic insights of their influences on tissue regeneration.

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Comparative Studies to Evaluate Relative in vitro Potency of Luteolin in Inducing Cell Cycle Arrest and Apoptosis in HaCaT and A375 Cells

Cited by 51 publications

References 42 publications

Luteolin Arrests Cell Cycling, Induces Apoptosis and Inhibits the JAK/STAT3 Pathway in Human Cholangiocarcinoma Cells

Luteolin Arrests Cell Cycling, Induces Apoptosis and Inhibits the JAK/STAT3 Pathway in Human Cholangiocarcinoma Cells

Luteolin-loaded Phytosomes Sensitize Human Breast Carcinoma MDA-MB 231 Cells to Doxorubicin by Suppressing Nrf2 Mediated Signalling

Mechanistic Insights of Cells in Porous Scaffolds via Integrated Culture Technologies

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