Comparative Studies on the Physico-chemical Characteristics of Bio-materials with Collagen from Calf and Fish Skins from Black Sea

Abstract

Collagen hydrolysers are three-dimensional polymeric materials with limited cross-linking and high hydrophilicity, having multiple medical applications. The most used collagen is the one extracted from bovine skin, which is now the industrial source of collagen. Due to the outbreak of some threatening diseases such as BSE, transmissible spongiform encephalopathy, foot-and-mouth disease, researchers have sought a safer alternative to collagen. This was the marine resource, which offered multiple opportunities to capitalize on clean sea-water raw material. This paper presents a comparative study of the physico-chemical properties of collagen hydrogels derived from collagen obtained from calf and skin from the Black Sea. Physico-chemical and spectrophotometric analyzes were performed to determine the structure. Studies have been conducted to analyze rheological behavior, antioxidant activity and antimicrobial activity. The total antioxidant capacity (ACL) is higher for collagen mixtures with 40% ethyl alcohol and shows higher values for fish collagen compared to calf collagen. Antimicrobial analysis shows that all collagen hydrogels show antimicrobial activity, both gram-positive (Staphylococcus aureus ATCC 6538P) and gram-negative (Escherichia coli ATCC 10536), which increases with increasing collagen concentrations.

Structural and molecular characterization of collagen‐type I extracted from lamb feet

“…In our study, longer enzymatic treatment (24 h) with sonication resulted in higher collagen concentrations. The collagen content of LF was comparable with the calf skin collagen (922 ± 1 mg/g) (Cherim et al., 2019) and had higher collagen content than silky fowl feet collagen (516.9 ± 31.2 mg/g) (Cheng et al., 2009), soft‐shelled turtle calipash collagen (509 ± 8 mg/g) (Zou et al., 2017), and golden carp skin collagen (512.65 ± 19.95 mg/g) (Ali et al., 2018). These results demonstrate the potential of utilizing an ultrasonic probe to enhance collagen extraction yield from LF.…”

Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

“…Besides the interspecies difference, collagen is a material of natural origin, thus its properties may vary depending on production batch, purification procedure, or storage conditions [ 41 , 42 , 43 ]. Several concerns over the use of collagen of bovine origin have already been raised, mainly connected to possible transmission of bovine spongiform encephalopathy (BSE) or viruses [ 37 , 44 ]. Thus, novel sources of collagen are being developed, including fish skin, jelly fish, plants, or synthetic KOD (a synthetic analogue of collagen composed of 36 amino acids organized into triple-helixes) [ 45 , 46 ].…”

Quantitative Analysis of Polyphenols and Biological Activity of Sage Macerates

“…The antioxidant capacity was evaluated using DPPH Radical Scavenging test. Gallic acid (GA) was used as standard to plot calibration curves and the results were expressed as equivalents (mg GAE) [14,16,[27][28][29][30][31][32]. In 25 mL calibrated flasks different volume of gallic acid solutions were added, then 5 mL DPPH 0.063% (1.268 mM) in methanol, filled up to the mark with methanol and let in the dark, to the room temperature for 45 minutes before the absorbance registration at 530 nm versus methanol.…”