Comparative studies on the immune system of fishes and amphibians: antigen localization in the carp <i>Cyprinus carpio</i> L.

Secombes, Chris J.; Manning, Margaret J.

doi:10.1111/j.1365-2761.1980.tb00424.x

Cited by 83 publications

(33 citation statements)

References 20 publications

(19 reference statements)

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“…Comparative studies on antigen localization in carp (C'prinus carpio) [28] and plaice [28] demonstrate the importance of the kidney in trapping both soluble and particulate antigens, although there was no attempt to quantify the results. In addition to the kidney, however, they stressed the importance of the spleen, particularly the reticulin sheaths of the ellipsoids.…”

Section: Discussionmentioning

confidence: 99%

The Quantitative Clearance of Bacteria from the Bloodstream of Rainbow Trout (Salmo gairdneri)

et al. 1982

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Abstract. The blood clearance of 51Cr-labelled heat-killed Salmonella pullorurn was biphasic and exponential for each phase. This pattern was the same for all three concentrations of bacteria used. The initial more rapid phase lasted for approximately 15 minutes when roughly 90% of the bacteria had been cleared. The second slower phase started 25 to 30 minutes post-inoculation and lasted for approximately 25 to 30 minutes. The tissue distribution of bacteria showed that in both absolute and relative terms, the kidney was the most important organ, accounting for more than 70% uptake of the bacteria.These kinetics demonstrate that rainbow trout at 8°C are able to clear their bloodstream of Salmonella very efficiently, in a fashion very similar to mammals. It is not known, however, which of the various possible clearance mechanisms dominates each phase.Fish are becoming increasingly important both in terms of their farming potential (aquaculture) and the possibility of their use as sentinels for detecting environmental pollution. Many of the more economically serious diseases of fish are bacterial, and yet little is known about the in vivo fate of su:h pathogens.Many workers have emphasized the efficiency of the reticuloendothelial system in clearing the bloodstream [27] and the kinetics of the clearance of particulate matter (both inert colloids and bacteria) usually has been accepted as a direct measure of this phagocytic efficiency [29]. Despite substantial work on reticuloendothelial kinetics in mammals, there is little knowledge of the subject in teleost fish, or ectothermic vertebrates in general. Indeed, we can find only one report describing in vivo rates of reticuloendothelial clearance in fish-in the goldfish (Curussius uurutus), lamprey (PetromyconJluviutiZis)7 and dogfish (Scylliorhinus caniculus) [25].The histological and ultrastructural location and distribution of intravenously and intraperitoneally injected vital dyes and other particles have been studied in several fish species [lo, 11, 17,23, 24,3 I]. These studies represent in broad terms a "mapping outy7 of the fish reticuloendothelial system in both fresh and saltwater species, ranging from primitive to advanced members of the group.Briefly, these studies (largely using colloidal carbon) showed that in teleosts, the spleen and kidney (both anterior and posterior) were important reticuloendothelial 687

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Section: Discussionmentioning

confidence: 99%

The Quantitative Clearance of Bacteria from the Bloodstream of Rainbow Trout (Salmo gairdneri)

et al. 1982

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“…Various circulating particulate and soluble antigenic and nonantigenic substances have been shown to be rapidly trapped in the sinusoids of the head kidney and in the ellipsoids of the spleen (Ellis et al, 1976;Ellis, 1980;Secombes & Manning, 1980;Dannevig & Berg, 1986;Espenes et al, 1995a).…”

Section: Introductionmentioning

confidence: 99%

The trapping of intravenously injected extracellular products fromAeromonas salmonicidain head kidney and spleen of vaccinated and nonvaccinated Atlantic salmon,Salmo salarL.

Espenes

Press

Reitan

et al. 1996

Fish & Shellfish Immunology

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“…The fate of IP injected bacterin was studied in plaice by ELLIS, et al (1976) and in gold fish by SECOMBES and MANNING (1980). Injected antigen was accumulated by the ellipsoids of the spleen and then migrated to the melanomacrophage centers (FERGUSON, 1976).…”

Section: Introductionmentioning

confidence: 99%

Tissue location of Vibrio bacterin delivered by intraperitoneal injection, immersion and oral routes to Salmo gairdneri.

1985

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The tissue location of Vibrio anguillarum bacterin in rainbow trout was studied using three delivery methods : intraperitoneal (IP) injection, immersion and per os. The fate of the bacterin was determined using histologically sectioned trout stained with anti-V. anguillarum (strain LS-174) fluorescein-labeled, purified, rabbit immunoglobulin G (IgG). The bacterin administered IP was accumulated by macrophages and observed in the kidney and spleen 28 d after injection. Bacterin delivered by immersion was observed on the surface of the gills, and in the gastrointestinal tract and kidney. Vaccine administered by the oral route was not detected systemically in test fish; the only accumulations found were in the lamina epithelialis of the lower intestine. Immunity developed with bacterins administered either by IP injection or immersion was considered to be systemic, because after vaccination, antigen was observed in a variety of internal fish tissues. Oral immunization apparently caused induction of a mucosal response because no antigen was observed in organs other than the intestine.

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Comparative studies on the immune system of fishes and amphibians: antigen localization in the carp Cyprinus carpio L.

Cited by 83 publications

References 20 publications

The Quantitative Clearance of Bacteria from the Bloodstream of Rainbow Trout (Salmo gairdneri)

The Quantitative Clearance of Bacteria from the Bloodstream of Rainbow Trout (Salmo gairdneri)

The trapping of intravenously injected extracellular products fromAeromonas salmonicidain head kidney and spleen of vaccinated and nonvaccinated Atlantic salmon,Salmo salarL.

Tissue location of Vibrio bacterin delivered by intraperitoneal injection, immersion and oral routes to Salmo gairdneri.

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