Comparative studies on the fish-killing activities of <i>Chattonella marina</i> isolated in 1985 and <i>Chattonella antiqua</i> isolated in 2010, and their possible toxic factors

Cho, Kichul; Sakamoto, Jun; Noda, Tatsuki; Nishiguchi, Tomoki; Ueno, Munetaka; Yamasaki, Yasuhiro; Yagi, Minoru; Kim, Daekyung; Oda, Tatsuya

doi:10.1080/09168451.2015.1116929

Cited by 17 publications

(13 citation statements)

References 36 publications

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“…1 ; Supplementary Table S1 ). The major ROS superoxide ( Oda et al , 1997 ; Marshall et al , 2005a ; Portune et al , 2010 ; Mooney et al , 2011 ; Dorantes-Aranda et al , 2015 ; Schneider et al , 2016 ; Cho et al , 2017 ), hydrogen peroxide ( Oda et al , 1997 ; Kim et al , 1999a ; Portune et al , 2010 ; Schneider et al , 2016 ; Cho et al , 2017 ) and hydroxyl radical ( Oda et al , 1992a ; Yang et al , 1995 ; Cho et al , 2016 ) have all been examined in phytoplankton. Compared to previous studies on hydroxyl radical production, however, the literature on superoxide and hydrogen peroxide generation by phytoplankton is much more expansive.…”

Section: Survey Of Extracellular Ros Production By Phytoplanktonmentioning

confidence: 99%

Production of extracellular reactive oxygen species by phytoplankton: past and future directions

Diaz

Plummer

2018

Journal of Plankton Research

103

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In aquatic environments, phytoplankton represent a major source of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. Many phytoplankton taxa also produce extracellular ROS under optimal growth conditions in culture. However, the physiological purpose of extracellular ROS production by phytoplankton and its wider significance to ecosystem-scale trophic interactions and biogeochemistry remain unclear. Here, we review the rates, taxonomic diversity, subcellular mechanisms and functions of extracellular superoxide and hydrogen peroxide production by phytoplankton with a view towards future research directions. Model eukaryotic phytoplankton and cyanobacteria produce extracellular superoxide and hydrogen peroxide at cell-normalized rates that span several orders of magnitude, both within and between taxa. The potential ecophysiological roles of extracellular ROS production are versatile and appear to be shared among diverse phytoplankton species, including ichthyotoxicity, allelopathy, growth promotion, and iron acquisition. Whereas extracellular hydrogen peroxide likely arises from a combination of intracellular and cell surface production mechanisms, extracellular superoxide is predominantly generated by specialized systems for transplasma membrane electron transport. Future insights into the molecular-level basis of extracellular ROS production, combined with existing high-sensitivity geochemical techniques for the direct quantification of ROS dynamics, will help unveil the ecophysiological and biogeochemical significance of phytoplankton-derived ROS in natural aquatic systems.

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Section: Survey Of Extracellular Ros Production By Phytoplanktonmentioning

confidence: 99%

Production of extracellular reactive oxygen species by phytoplankton: past and future directions

Diaz

Plummer

2018

Journal of Plankton Research

103

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“…These findings should therefore be verified by using a single experimental design to accurately narrow down the parameters responsible for the mortality of aquacultured fish. The toxicity of Chattonella may depend on culture strain [12], although it is unclear if there are significant differences in toxicity among the former three species classified by morphology, such as cell size [33]. A comparative study using strains with different ichthyotoxicity could be effective for identifying the toxic parameter(s) of Chattonella.…”

Section: Introductionmentioning

confidence: 99%

Superoxide Production by the Red Tide-Producing Chattonella marina Complex (Raphidophyceae) Correlates with Toxicity to Aquacultured Fishes

Shikata¹,

Yuasa

Kitatsuji

et al. 2021

Antioxidants

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The marine raphidophyte Chattonella marina complex forms red tides, causing heavy mortalities of aquacultured fishes in temperate coastal waters worldwide. The mechanism for Chattonella fish mortality remains unresolved. Although several toxic chemicals have been proposed as responsible for fish mortality, the cause is still unclear. In this study, we performed toxicity bioassays with red sea bream and yellowtail. We also measured biological parameters potentially related to ichthyotoxicity, such as cell size, superoxide (O2•−) production, and compositions of fatty acids and sugars, in up to eight Chattonella strains to investigate possible correlations with toxicity. There were significant differences in moribundity rates of fish and in all biological parameters among strains. One strain displayed no ichthyotoxicity even at high cell densities. Strains were categorized into three groups based on cell length, but this classification did not significantly correlate with ichthyotoxicity. O2•− production differed by a factor of more than 13 between strains at the late exponential growth phase. O2•− production was significantly correlated with ichthyotoxicity. Differences in fatty acid and sugar contents were not related to ichthyotoxicity. Our study supports the hypothesis that superoxide can directly or indirectly play an important role in the Chattonella-related mortality of aquacultured fishes.

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“…The raphidophycean flagellates Chattonella marina and C. ovata [1][2][3][4][5], and other flagellates such as Heterosigma akashiwo [6], Heterocapsa circularisquama [7], Phaeocystis globosa [8], Amphidinium carterae [9,10], Prymnesium parvum, and Chrysochromulina polylepis [11] have been reported as the causative species of massive, fish-killing algal blooms worldwide. The major ichthyotoxic effects of these flagellates were identified as: (1) producing reactive oxygen species (ROS) [5,[12][13][14][15], (2) clogging of the gills [4,[16][17][18], (3) causing neurotoxininduced cardiac disorders [19], and (4) producing hemolytic toxins [20][21][22][23] that result in necrosis of the gills. All these effects function either separately or synergistically, resulting in gill tissue injury or direct/indirect toxicity to the fish.…”

Section: Introductionmentioning

confidence: 99%

Hemolytic Activity in Relation to the Photosynthetic System in Chattonella marina and Chattonella ovata

Tong

Gou

et al. 2021

Marine Drugs

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Chattonella species, C. marina and C. ovata, are harmful raphidophycean flagellates known to have hemolytic effects on many marine organisms and resulting in massive ecological damage worldwide. However, knowledge of the toxigenic mechanism of these ichthyotoxic flagellates is still limited. Light was reported to be responsible for the hemolytic activity (HA) of Chattonella species. Therefore, the response of photoprotective, photosynthetic accessory pigments, the photosystem II (PSII) electron transport chain, as well as HA were investigated in non-axenic C. marina and C. ovata cultures under variable environmental conditions (light, iron and addition of photosynthetic inhibitors). HA and hydrogen peroxide (H2O2) were quantified using erythrocytes and pHPA assay. Results confirmed that% HA of Chattonella was initiated by light, but was not always elicited during cell division. Exponential growth of C. marina and C. ovata under the light over 100 µmol m−2 s−1 or iron-sufficient conditions elicited high hemolytic activity. Inhibitors of PSII reduced the HA of C. marina, but had no effect on C. ovata. The toxicological response indicated that HA in Chattonella was not associated with the photoprotective system, i.e., xanthophyll cycle and regulation of reactive oxygen species, nor the PSII electron transport chain, but most likely occurred during energy transport through the light-harvesting antenna pigments. A positive, highly significant relationship between HA and chlorophyll (chl) biosynthesis pigments, especially chl c2 and chl a, in both species, indicated that hemolytic toxin may be generated during electron/energy transfer through the chl c2 biosynthesis pathway.

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Comparative studies on the fish-killing activities of Chattonella marina isolated in 1985 and Chattonella antiqua isolated in 2010, and their possible toxic factors

Cited by 17 publications

References 36 publications

Production of extracellular reactive oxygen species by phytoplankton: past and future directions

Production of extracellular reactive oxygen species by phytoplankton: past and future directions

Superoxide Production by the Red Tide-Producing Chattonella marina Complex (Raphidophyceae) Correlates with Toxicity to Aquacultured Fishes

Hemolytic Activity in Relation to the Photosynthetic System in Chattonella marina and Chattonella ovata

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