Comparative studies on ectopic bone formation in porous hydroxyapatite scaffolds with complementary pore structures

Wang, Hao; Zhi, Wei; Li, Xiong; Li, Xiaohong; Duan, Ke; Duan, Rongquan; Mu, Yandong; Weng, Jie

doi:10.1016/j.actbio.2013.05.026

Cited by 90 publications

(83 citation statements)

References 35 publications

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“…Hydroxyapatite-based scaffolds have been known to induce bone formation in the muscle when used alone in larger animal models, thus corroborating well with our observations. 33 However, the amount of time required for mineralisation in ectopic sites has always been longer than that which we have observed in our clinical case. In our opinion, this is an additional effect observed due to the presence of a ceramic matrix and bone active proteins leaking out of the bone cavity.…”

Section: Discussioncontrasting

confidence: 45%

Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial

Raina

Gupta

Petersen

et al. 2016

Bone & Joint Research

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ObjectivesWe have observed clinical cases where bone is formed in the overlaying muscle covering surgically created bone defects treated with a hydroxyapatite/calcium sulphate biomaterial. Our objective was to investigate the osteoinductive potential of the biomaterial and to determine if growth factors secreted from local bone cells induce osteoblastic differentiation of muscle cells.Materials and MethodsWe seeded mouse skeletal muscle cells C2C12 on the hydroxyapatite/calcium sulphate biomaterial and the phenotype of the cells was analysed. To mimic surgical conditions with leakage of extra cellular matrix (ECM) proteins and growth factors, we cultured rat bone cells ROS 17/2.8 in a bioreactor and harvested the secreted proteins. The secretome was added to rat muscle cells L6. The phenotype of the muscle cells after treatment with the media was assessed using immunostaining and light microscopy.ResultsC2C12 cells differentiated into osteoblast-like cells expressing prominent bone markers after seeding on the biomaterial. The conditioned media of the ROS 17/2.8 contained bone morphogenetic protein-2 (BMP-2 8.4 ng/mg, standard deviation (sd) 0.8) and BMP-7 (50.6 ng/mg, sd 2.2). In vitro, this secretome induced differentiation of skeletal muscle cells L6 towards an osteogenic lineage.ConclusionExtra cellular matrix proteins and growth factors leaking from a bone cavity, along with a ceramic biomaterial, can synergistically enhance the process of ectopic ossification. The overlaying muscle acts as an osteoinductive niche, and provides the required cells for bone formation.Cite this article: D. B. Raina, A. Gupta, M. M. Petersen, W. Hettwer, M. McNally, M. Tägil, M-H. Zheng, A. Kumar, L. Lidgren. Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial. Bone Joint Res 2016;5:500–511. DOI: 10.1302/2046-3758.510.BJR-2016-0133.R1.

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Section: Discussioncontrasting

confidence: 45%

Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial

Raina

Gupta

Petersen

et al. 2016

Bone & Joint Research

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“…Scaffold choice is still under investigation in order to be standardized. Biodegradable synthetic polyesters [73], calcium phosphate ceramics [74,75] and chitosan–alginate [76] are some of the scaffolds that have proved to have significant value in the treatment of bone defects.…”

Section: Introduction: Fundamentals Of Tissue Engineering and Regenermentioning

confidence: 99%

Current concepts: tissue engineering and regenerative medicine applications in the ankle joint

Correia

Pereira

Silva‐Correia

et al. 2014

J. R. Soc. Interface.

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Tissue engineering and regenerative medicine (TERM) has caused a revolution in present and future trends of medicine and surgery. In different tissues, advanced TERM approaches bring new therapeutic possibilities in general population as well as in young patients and high-level athletes, improving restoration of biological functions and rehabilitation. The mainstream components required to obtain a functional regeneration of tissues may include biodegradable scaffolds, drugs or growth factors and different cell types (either autologous or heterologous) that can be cultured in bioreactor systems (in vitro) prior to implantation into the patient. Particularly in the ankle, which is subject to many different injuries (e.g. acute, chronic, traumatic and degenerative), there is still no definitive and feasible answer to 'conventional' methods. This review aims to provide current concepts of TERM applications to ankle injuries under preclinical and/or clinical research applied to skin, tendon, bone and cartilage problems. A particular attention has been given to biomaterial design and scaffold processing with potential use in osteochondral ankle lesions.

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“…After a sharp decrease at the initial stage, the current density stabilizes at approximately 0.15 A/cm 2 . Pure HA coating forms at room temperature when the current density is greater than 0.01 A/cm 2 [13].…”

Section: Ha1mentioning

confidence: 99%

“…The surface of HA coatings with plate-like and porous structures can provide a relatively large surface area for improving chemical interaction with the biological environment. Furthermore, the porous structure of HA coatings is an important factor in implant fixation by bone ingrowths into pores [12][13][14]. The electrochemical deposition method (EDM) is widely used to deposit HA coatings because of its high deposition rate at low temperatures [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Effect of Na+ and NaOH concentrations on the surface morphology and dissolution behavior of hydroxyapatite

Mao

Yang

Zhu

et al. 2015

Ceramics International

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Comparative studies on ectopic bone formation in porous hydroxyapatite scaffolds with complementary pore structures

Cited by 90 publications

References 35 publications

Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial

Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial

Current concepts: tissue engineering and regenerative medicine applications in the ankle joint

Effect of Na+ and NaOH concentrations on the surface morphology and dissolution behavior of hydroxyapatite

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