Comparative studies on chromatin proteins from chicken thrombocytes and erythrocytes

Krajewska, Wanda M.; Klyszejko-Stefanowicz, L

doi:10.1016/0005-2795(80)90093-8

Cited by 4 publications

(3 citation statements)

References 30 publications

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“…Contrary to erythrocytes, the absence of previous studies of the lipid composition of thrombocytes in birds precludes any comparison of our findings with data for other avian species. Although thrombocytes and erythrocytes have been suggested to be genealogically closely related in birds (10), our results do not support this hypothesis since each cell type has its own PL distribution and FA composition. Although the possibility of late biochemical differentiation in the circulating blood cannot be excluded, it would seem unlikely that avian erythrocytes and thrombocytes derive from closely related cells.…”

Section: Discussioncontrasting

confidence: 99%

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Lipid composition of erythrocytes and thrombocytes of a subantarctic seabird, the king penguin

Fayolle

Leray

Ohlmann

et al. 2000

Lipids

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Phospholipid (PL) compositions and fatty acid (FA) patterns of PL were determined in the erythrocytes and blood thrombocytes of a seabird, the king penguin, living in the subantarctic area and feeding on prey rich in n-3 polyunsaturated FA. Results were compared between birds in three different physiological states (breeding and molting adults, chicks) to those reported for other birds. In erythrocytes, the ratios of cholesterol to PL and of sphingomyelin to phosphatidylcholine (PC) were lower than in other birds. The PL distribution was similar to those previously reported in the hen and pigeon. In contrast to other birds, cardiolipin levels were unexpectedly high (4%). Very long chain n-3 FA were abundant (13-27%) in phosphatidylethanolamine (PE), phosphatidylserine and PC, probably in relation to the natural diet of these birds. Among n-3 FA, 22:6n-3 was the most abundant in all PL (2-20%), whereas the highest levels of arachidonic acid were observed in PE (14%). In thrombocytes, the PL distribution and FA composition of the main PL (PC, PE) differed from those of erythrocytes, and in particular, levels of n-3 FA (9-12%) were 1.5-2 times lower. The highest levels of arachidonic acid were found in phosphatidylinositol (24%). The lipid profile of penguin erythrocytes could contribute to the efficiency of blood circulation and oxygen delivery in microvascular beds, thus favoring diving capacity of these animals. Our observations do not support the hypothesis of a common origin of avian thrombocytes and erythrocytes.

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Section: Discussioncontrasting

confidence: 99%

“…In addition to hemosta-sis, these cells have been reported to play an active role in phagocytosis (7,8) but are not a component of the lymphocyte system (9). Finally, avian thrombocytes and erythrocytes share many similarities, and the two cell types are thought to be derived from a common progenitor cell line (10).…”

mentioning

confidence: 99%

Lipid composition of erythrocytes and thrombocytes of a subantarctic seabird, the king penguin

Fayolle

Leray

Ohlmann

et al. 2000

Lipids

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“…Moreover, analysis of histone H5 expression was also performed. H5 is a chromatin protein that is specific for the erythrocytic and megakaryocytic lineage (32). As shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

v-jun cooperates with v-erbB to transform the thrombocytic/megakaryocytic lineage.

Garcia

Samarut

1993

Proc. Natl. Acad. Sci. U.S.A.

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The transforming properties of v-jun, the viral counterpart of the transcription factor AP1, were investgated in avian hematopoletic cells. Two retroviruses, called JB and JBN, expressing both v-jun and v-erbB, were constructed using an avian erythroblastosls-based vector. We show that the cooperative action of both oncogenes allowed the virus to efficiently transform bone marrow cells. No such transformation was obtained with either oncogene alone. JBtransformed bone marrow cells expressed GATA-1, TAL-1, and histone H5, suggesting that they belong to the erythrocytic/thrombocytic llneage. (Thrombocytes are the avian homologues of mammal megakaryocytes.) Moreover, after induction with phorbol 12-myristate 13-acetate JB-trnformed bone marrow cells began to differentiate and synthesized hig levels of platelet glycoproteins, indicating that they were of thrombocytic origin. These results were confirmed by c-etsl analysis since this transcription factor, specifically found in cells with megakaryocytic but not erythrocytic features, was clearly detected in these cells.

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Immunospecificity of nonhistone chromatin proteins tightly bound to DNA from chicken thrombocytes and erythrocytes

Krajewska

Klyszejko-Stefanowicz

1982

Mol Biol Rep

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Erythroid cell-specific antisera capable of detecting tightly bound nonhistone chromatin protein--DNA complexes were obtained by injecting rabbits with dehistonized chicken reticulocyte chromatin. The antisera showed no crossreactivity with chromatin of thrombocytes which are regarded as cells genealogically closely related with erythrocytes. The lack of thrombocyte chromatin immunoactivity was not caused by conformational constrains. Tightly bound nonhistone protein--DNA complexes isolated from thrombocyte chromatin showed no immunological similarity with these of erythrocyte chromatin.

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Comparative studies on chromatin proteins from chicken thrombocytes and erythrocytes

Cited by 4 publications

References 30 publications

Lipid composition of erythrocytes and thrombocytes of a subantarctic seabird, the king penguin

Lipid composition of erythrocytes and thrombocytes of a subantarctic seabird, the king penguin

v-jun cooperates with v-erbB to transform the thrombocytic/megakaryocytic lineage.

Immunospecificity of nonhistone chromatin proteins tightly bound to DNA from chicken thrombocytes and erythrocytes

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