Comparative Studies on Acid Phosphatase Isozymes of
Developing Human Placenta and Fetal Liver

Kushari, J.; Mukherjea, Manju

doi:10.1159/000459273

Cited by 3 publications

(2 citation statements)

References 4 publications

(4 reference statements)

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“…Gestational age was calculated from the period of amenorrhea and by generally provided data correct to ± 1 week [13]. The tissues were kept in ice and processed at 0-4 °C.…”

Section: Methodsmentioning

confidence: 99%

Action of Polyamines on Ribonucleic Acid and Protein Synthesis during Ontogeny of Human Fetal Liver

Choudhury¹,

Chaudhuri²,

Kushari³

et al. 1984

Neonatology

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A higher rate of RNA synthesis in human fetal liver cells was found during the early age of gestation, after a drop, it gradually increases to a maximum value between 18 and 22 weeks, followed by a sharp decrease at later period of gestation. Total polyamine content of fetal liver tissue shows a similar trend. However, when liver cell suspension was incubated with exogenous spermine, spermidine and putrescine there is a dose-dependent inhibition in RNA synthesis. Protein synthesis in human fetal liver cells was stimulated with lower doses of spermine, spermidine and putrescine and inhibited at higher doses. The optimum dose for stimulation and the degree of stimulation was, however, not the same for fetuses of different gestational ages.

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“…Gestational age was calculated from the period of amenorrhea and by generally provided data correct to ± 1 week [13]. The tissues were kept in ice and processed at 0-4 °C.…”

Section: Methodsmentioning

confidence: 99%

Action of Polyamines on Ribonucleic Acid and Protein Synthesis during Ontogeny of Human Fetal Liver

Choudhury¹,

Chaudhuri²,

Kushari³

et al. 1984

Neonatology

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“…The results are shown in figure 5 and table II. The Al and A2 phosphatases turned out to be electrophoresis [3] and three fractions preparatively on Sepharose G-200 [4]. The present study describes the separation of pla cental acid phosphatases based on affinity chromatography.…”

Section: Characterizationmentioning

confidence: 98%

Acid Phosphatases of the Human Placenta, Characterization and Immunological Comparison with Prostatic Acid Phosphatase

Skinningsrud¹

1983

Enzyme

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Four different acid phosphatases, denoted A1, A2, B and C, were separated from human placental homogenates. The enzymes A1, A2 and B were separated from enzyme C by binding to concanavalin A-Sepharose. Although the A1, A2 and B enzymes were all strongly inhibited by L-tartrate, only the A1 enzyme bound by affinity chromatography to L-tartrate- Sepharose. The A2 and B enzymes were separated on DEAE-cellulose. A1, A2 and B had molecular weights about 95,000. Enzyme B had high K(M), whereas enzymes A1 and A2 had low K(m). The enzymes A1 and A2 bound to antibodies raised against prostatic acid phosphatase, whereas the enzymes B and C did not.

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Properties of a partially purified acid phosphatase from pathogenic Nocardia brasiliensis

1992

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Like many other bacteria, Nocardia sp. possess acid phosphatase activity. In N. brasiliensis, a human and animal pathogen, this activity was resolved into two enzyme forms by native gel electrophoresis. One (isozyme I) was partially purified and characterized. It exhibited an estimated molecular weight on SDS-PAGE of 50 kDa, a pH optimum of 5.2, and a Km value of 1.25 mM for p-nitrophenylphosphate. The N. brasiliensis enzyme was stable at 4 degrees C for at least 24 h, but readily inactivated at 60 degrees C. Ammonium molybdate, sodium fluoride and L-(+)-tartrate were found to be potent inhibitors of the enzyme. Although its function is presently unknown, by analogy to other bacterial systems it could be envisioned to play an important role in the physiology and pathogenicity of the microorganism.

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Comparative Studies on Acid Phosphatase Isozymes of Developing Human Placenta and Fetal Liver

Cited by 3 publications

References 4 publications

Action of Polyamines on Ribonucleic Acid and Protein Synthesis during Ontogeny of Human Fetal Liver

Action of Polyamines on Ribonucleic Acid and Protein Synthesis during Ontogeny of Human Fetal Liver

Acid Phosphatases of the Human Placenta, Characterization and Immunological Comparison with Prostatic Acid Phosphatase

Properties of a partially purified acid phosphatase from pathogenic Nocardia brasiliensis

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