Comparative Studies of Various Molecular Species inAspergillus nigerGlucoamylase

Effective Purification of Glucoamylase inKoji, a Solid Culture ofAspergillus oryzaeon Steamed Rice, by Affinity Chromatography Using an Immobilized Acarbose (BAY g-5421)

“…The inconsistency might reflect some anomalous electrophoretic and hydrodynamic behaviors of the GA as an acidic glycoprotein. 6 ) The enzyme sedimented as a uniform species sisted of glucosamine, mannose, and galactose. The numbers of the residues in the OA molecule are presented in Table III.…”

“…After acid hydrolysis of GA, the liberated sugars were converted to the corresponding aldononitrile acetate to analyze the chemical composition with GLC/MS, lEOL model DX-300. 6 ) on sedimentation analysis, with an S20, w value of 4.96. Weight-average molecular weight (MW) of the GA was 79.5 k by sedimentation equilibrium.…”

“…In a single chromatographic process on the affinity column, only GAs were trapped collectively from a commercially available crude enzyme preparation. 5 , 6) In this study, the affinity chromatography using immobilized acarbose was applied to purifying GA from koji, unlike the commercial enzyme preparation, and it was proved that A. oryzae GA was purified by one cycle of the affinity chromatography. Thus, the enzymatic properties of the purified GA are also provided.…”

Microbial Enzymes and Biotechnology

“…Svensson et al (1982) resolved the active components from a commercial preparation into two forms, named G I and G II. Five forms were fully characterised (Ono et al, 1988b) and differed in relative molecular mass, sedimentation constant, chemical composition, isoelectric point, kinetic parameters and other enzymatic properties. The amino acid content of the two forms were very similar.…”

Molecular Cloning of the Glucoamylase Gene ofAspergillus shirousamiand Its Expression inAspergillus oryzae

“…PolyA h3' -100 ---50"" -I I' tat 8 a ae t gaci t II t cgc t caglll t cae t g'lllll t I.,ac t "ge t tee teeec etc a agaegegacaac tgae t g.e t lagc t t ca tee t caa cat c g t t ae t eccccacclc a 10 20 AN GPALRATAMIGFGOWLLONGYTSTATOIVWPLVRNOLSYVAOYWNOTGYO AS " , " , " " " " , " , , " " A, 'E"" ,"'" , , , , " " "'"…”