Comparative studies of the toxicity of standard reference materials in various cytotoxicity tests and <i>in vivo</i> implantation tests

Tsuchiya, Toshie; Ikarashi, Yoshiaki; Hata, Hideyo; Toyoda, Kazuhiro; Takahashi, Michihito; Uchima, Takao; Tanaka, Noriho; Sasaki, Toshi; Nakamura, Akitada

doi:10.1002/jab.770040206

Cited by 25 publications

(11 citation statements)

References 4 publications

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“…The use of ZCF as cytotoxic control has been established previously for multiple cell types with varying cytotoxic responses 20,21,31,32 . L929 cells had the greatest cytotoxic response to ZCF when compared to the MC3T3, hMSC and cMSC cells.…”

Section: Experimental Section: Discussionmentioning

confidence: 99%

Evaluation of the In Vitro Cytotoxicity of Cross-Linked Biomaterials

et al. 2013

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This study evaluated the in vitro cytotoxicity of poly(propylene fumarate) (PPF). PPF is an aliphatic biodegradable polymer that has been well characterized for use in bone tissue engineering scaffolds. Four different cell types, human mesenchymal stem cells (hMSC), fibroblasts (L929), pre-osteoblasts (MC3T3), and canine mesenchymal stem cells (cMSC), were used to evaluate the cytotoxicity of PPF. These cell types represent the tissues that PPF would interact with in vivo as a bone tissue scaffold. The sol fraction of the PPF films was measured and then utilized to estimate crosslinking density. Cytotoxicity was evaluated using XTT assay and fluorescence imaging. Results showed that PPF supported similar cell metabolic activities of hMSC, L929, MC3T3 and cMSC compared to the non-cytotoxic control, high density polyethylene (HDPE) and were statistically different than those cultured with the cytotoxic control, a polyurethane film containing 0.1% zinc diethyldithiocarbamate (ZCF). Results showed differing cellular responses to ZCF, the cytotoxic control. The L929 cells had the lowest cell metabolic activity levels after exposure to ZCF compared to the cell metabolic activity levels of the MC3T3, hMSC or cMSC cells. Qualitative verification of the results using fluorescence imaging demonstrated no change in cell morphology, vacuolization, or detachment when cultured with PPF compared to HDPE or blank media cultures. Overall the cytotoxicity response of the cells to PPF was demonstrated to be similar to the cytotoxic response of cells to known non-cytotoxic materials (HDPE).

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Section: Experimental Section: Discussionmentioning

confidence: 99%

Evaluation of the In Vitro Cytotoxicity of Cross-Linked Biomaterials

et al. 2013

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“…Established cell lines are preferred and can be obtained from recognized repositories. Where a specific sensitivity is required, primary cell cultures and cell lines obtained directly from living tissues should only be used if reproducibility and accuracy of the response can be demonstrated 14-16. However, several problems, such as the preparation of test materials (in the case of the extract method), choice of cell type, the test procedure and method used to quantify results, etc., have been encountered in vitro cytotoxicity tests 17-19.…”

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confidence: 99%

Evaluation of the Extraction Method for the Cytotoxicity Testing of Latex Gloves

et al. 2005

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In this study, the cytotoxicity of medical latex gloves to cultured L-929 cells was determined using various extraction conditions. According to the extraction time and temperature, three types of extraction conditions were used: 1) 24 h at 37℃; 2) 72 h at 37℃; 3) 72 h at 50℃. Also, four different extraction vehicles were used, namely, distilled water (DW), 9 g/l sodium chloride (saline) in DW, and culture media with or without serum. Under the above-mentioned conditions, the samples were extracted and then 2-fold serially diluted in the concentration range 3.13 - 50%. When extracted with either DW or saline for 24 h or 72 h at 37℃, only 50% diluted samples showed distinct cytotoxicity to L-929 cells. Moreover, no cytotoxic potentials were observed when gloves were extracted with DW or saline at 50℃ for 72 h. Cytotoxicity was markedly greater when gloves were extracted with culture medium, irrespective of the presence of serum in the medium. These results suggest that optimal extraction conditions should be established for the cytotoxicity evaluations of biomaterials and medical devices.

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“…The reported results were in accordance with ours, even if the cell lines are different, they are both mice fibroblasts widely used in cytotoxicity tests. Tsuchiya et al . performed a comparative study of the toxicity of standard reference biomaterials using both L929 and Balb 3T3 cells and concluded that at the neutral red uptake assay, Balb 3 T3 presents higher sensitivity than L929 cells.…”

Section: Resultsmentioning

confidence: 99%

Silk fibroin membranes with self‐assembled globular structures for controlled drug release

Tomoda

Corazza

Beppu

et al. 2019

J of Applied Polymer Sci

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The cover image created by Mariana Agostini de Moraes and colleagues, represents the self-assembling of silk fibroin into globular structures within a continuous fibroin membrane matrix, encapsulating the drug model -diclofenac sodium. These structures were only observed when the drug was incorporated in the fibroin solution, prior to membrane preparation. The formation was spontaneous, and the globular structures presented regular size, shape and uniform distribution within the membrane. EDX mapping (red dots) of a fractured particle confirmed the high concentration of the drug within the globular structures. These results emphasize the potential use of silk fibroin for drug delivery applications.

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Comparative studies of the toxicity of standard reference materials in various cytotoxicity tests and in vivo implantation tests

Cited by 25 publications

References 4 publications

Evaluation of the In Vitro Cytotoxicity of Cross-Linked Biomaterials

Evaluation of the In Vitro Cytotoxicity of Cross-Linked Biomaterials

Evaluation of the Extraction Method for the Cytotoxicity Testing of Latex Gloves

Silk fibroin membranes with self‐assembled globular structures for controlled drug release

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