Comparative studies of physicochemical and biological properties between canine parvovirus and feline panleukopenia virus.

SUMMARYCanine parvovirus (CPV) strains were compared for haemagglutination (HA) activity and antigenicity and were divided into two types by HA activity. Strains Cp49 and 29-F showed temperature-dependent HA, like feline panleukopenia virus (FPLV) and mink enteritis virus (MEV), whereas strains Sp-80 and Y-1 showed temperatureindependent HA with erythrocytes from eight species of animals. The results of a cross HA inhibition test using immunized rat sera suggested that of the two types of CPV those showing temperature-dependent HA were antigenically like FPLV and MEV whereas those showing temperature-independent HA were not. This antigenic difference between the two types was confirmed by a HA inhibition test with monoclonal antibodies. A chronological survey revealed that CPV isolates from earlier years have HA activity and antigenicity similar to those of FPLV and MEV, whereas current CPV isolates do not. There are some exceptional isolates from the transitional period which have similar antigenicity to FPLV and MEV but different HA activity. These results suggest that the haemagglutinin of CPV altered from one form resembling that of FPLV to a somewhat different structure during passage in dogs in nature.

Section: Discussionmentioning

confidence: 96%

Canine Parvovirus: Strain Difference in Haemagglutination Activity and Antigenicity

Senda

Hirayama

Itoh

et al. 1988

“…Amino acid residues different from MEV-Abashiri except for these nine are indicated by arrowheads. DNA sequences of CPV-Kushiro (Goto et al, 1984), CPV-CP49 (Azetaka et aL, 1980) and CPV-obl were determined by direct sequencing of PCR products; those of MEV-A and -D, FPLV-Carl and FPLV-193 were reported by Parrish et al (1988a), Carlson et aL (1985) and Martyn et al (1990), respectively.…”

Section: Involvement Of Ns Genementioning

confidence: 99%

Mapping of determinants of the host range for canine cells in the genome of canine parvovirus using canine parvovirus/mink enteritis virus chimeric viruses

Horiuchi¹,

Gotō

Ishiguro³

et al. 1994

Feline panleukopenia virus (FPLV), mink enteritis virus (MEV) and canine parvovirus (CPV) are more than 98 % similar in DNA and predicted amino acid sequences, but they show different host-cell specificities; CPV is able to replicate in canine cells in culture, whereas FPLV and MEV cannot or replicate only to a low titre. To map the genomic region responsible for the host range of CPV in vitro, CPV/MEV chimeric viruses were generated by transfecting infectious CPV/MEV chimeric plasmids into a cultured feline kidney cell line, and their host cell ranges were analysed. The 60 to 91 map units (m.u.) region of the CPV genome, which contains a part of the capsid protein (VP) gene encoding from amino acid 91 (in the VP2 sequence) to the carboxy terminus of VP protein, was required to impart the ability to replicate in canine cells to MEV, although the chimeric virus containing the 60 to 91 m.u. region of the CPV genome in the MEV background did not replicate in canine cells as efficiently as did CPV derived from the infectious plasmid of CPV. Not only the VP gene, but also a part of the NS gene of CPV were considered to participate in the full expression of the ability to replicate in canine cells. Within the 60 to 91 m.u. region, five of nine amino acid changes between MEV-Abashiri and CPV-Y1 were thought to be phylogenetically CPV-common; however, a recombinant virus containing all five amino acid changes of CPV in the MEV background replicated minimally in canine cells.

“…Strain Abashiri of MEV (Higashihara et al, 1981) was propagated in FLF-3 cells, a feline lung fibroblast cell line, as described previously (Goto et al, 1984). MEV RF-DNAs, either free from (f-DNA) or with (p-DNA) the terminal proteins, were prepared from MEV-infected cells as described elsewhere (Shinagawa et al, 1989).…”

Section: Methodsmentioning

confidence: 99%

“…Although MEV, FPLV and CPV are closely related viruses, they are distinguishable from each other antigenically when examined with monoclonal antibodies (Parrish et al, 1982;Parrish & Carmichael, 1983;Surleraux et al, 1987), by thermoresistance and haemagglutination reactions (Carmichael et al, 1980;Goto et al, 1984), and by their growth characteristics in cultured ceils (Tratschin et al, 1982). It would contribute greatly to the understanding of the evolution of these viruses if the differences in these characteristics were shown to correspond to differences in nucleotide sequences.…”

Section: Introductionmentioning

confidence: 99%

Construction and nucleotide sequence analysis of an infectious DNA clone of the autonomous parvovirus, mink enteritis virus

Kariatsumari¹,

Horiuchi²,

Hama

et al. 1991

We have cloned the replicative form (RF-) DNA of mink enteritis virus (MEV), constructed an infectious recombinant plasmid containing MEV DNA and determined the nucleotide sequence of the clbned MEV DNA. RF-DNAs were detected and infectious virus was generated when the recombinant plasmid containing the entire MEV genome was introduced into feline kidney cell cultures. The MEV genome was 5094 nucleotides (nt) in length; the 3' end of the virion strand contained a 205 nt palindromic sequence and the 5' end a 62 nt palindromic sequence that could assume Y-and U-shaped configurations, respectively. The 5' end of the virion strand had a direct repeat of 61 nt at the carboxyl terminus of the capsid protein gene. The organization of the MEV genome is similar to those of canine parvovirus (CPV) and feline panleukopenia virus (FPLV); there are two large open reading frames (ORFs), one in the 3' half and the other in the 5' half of the genome, with coding capacities of 668 and 722 amino acid residues, respectively. Both are in the same reading frame and no significant ORFs are apparent in the virion strand (negative-sense strand). Possible functional promoter motifs are located at map unit (m.u.) 4-5 and m.u. 40, and a possible functional poly(A) signal is located at m.u. 96. The nucleotide and amino acid sequence homology with CPV and FPLV is greater than 98 %, consistent with the hypothesis that MEV and CPV are host-range variants of FPLV.