The HAIRS ABSENT (H) gene encodes the C2H2 zinc finger protein ZFP8, which affects trichome development. Subfamily analysis predicts that its homologous genes have a regulatory role in the development of trichomes in Solanaceous plants. This study found that overexpression of the H gene increased the levels of endogenous hormones cytokinin (CTK), auxin (IAA), jasmonic acid (JA), and gibberellin (GA) in tobacco, leading to plant dwarfism, inhibition of short-stalk glandular trichome (STG) development, promotion of long-stalk glandular trichome (LGT) development, increased trichome density, and enhanced tobacco's ability to resist insect predation. During the flowering phase, the H gene positively regulates NtHEMA, NtHLG2 and NtCAO to promote chlorophyll synthesis, and negatively regulates NtF3H, NtF3, H, NtDRF1, NtANS and NtFGT to inhibit anthocyanin synthesis, altering the contents of chlorophyll and anthocyanin, leading to the appearance of diversified flower colors. Besides, the H gene has a significant impact on plant cell development, causing the epidermal cells of the tobacco corolla to resemble those of the leaf epidermis, which gives the corolla a glossier color.