Comparative studies of genetic diversity in kenaf (Hibiscus cannabinus L.) varieties based on analysis of agronomic and RAPD data

Abstract

Kenaf (Hibiscus cannabinus L.) is a fiber crop classified in the genus Hibiscus (Malvaceae), and has a great potential for its multipurpose utilization, in addition to its traditional usage. Varietal identification of kenaf is always problematic and knowledge on genetic diversity of kenaf varieties is also limited, which significantly hindered our effective utilization and conservation of the valuable kenaf germplasm. In order to find a proper method for identifying kenaf varieties and studying their variation, morpho-agronomic characters and random amplified polymorphic DNA (RAPD) markers were analyzed among 14 kenaf varieties commonly used in Japan. Data from morphological analysis showed that the included kenaf varieties could be divided into three major groups. The characters, such as middle stem diameter, whole stalk weight, and days to 50% flowering, are highly responsible for the variation of the kenaf varieties, but it is difficult to identify individual varieties merely by the morpho-agronomic characters. On the other hand, clearly separation of the kenaf varieties was achieved based on the RAPD variation patterns. Genetic relationship of the kenaf varieties can also be traced through the analysis of RAPD and morph-agronomic variation. It is concluded from the present study that RAPD analysis is an effective tool in identifying of kenaf varieties and determining their genetic relationships, particularly when combined with the analysis of morpho-agronomic characters.

A population genetic study in a scattered forest species, wild service tree [Sorbus torminalis (L.) Crantz], using RAPD markers

“…Neutral genetic markers are also used in genetic differentiation analysis, but their reliability has been questioned when the evaluation of quantitative variation is an important research goal. Although it has been shown that the correlation between variation scored with such markers, and variation for adaptively important traits, is sometimes poor (Ennos et al 1997;Reed and Frankham 2001;Van Tienderen et al 2002;Bekessy et al 2003;Howe et al 2003) many studies have been successfully carried out to detect genetic diversity using such neutral markers (Cheng et al 2002;Hardy 2003;Shashidhara et al 2003).…”

Assessment of Genetic Variation in Different Kenaf (Hibiscus cannabinus) Genotypes using Morpho-agronomic Traits and RAPD Markers

“…To date, RAPD analysis has been effectively utilized for determining the genetic diversity in many species, for example, tomato (Joshi et al, 2013) and oat (Ruwali et al, 2013). It has also been reported that the superiority of the molecular analysis data than the morpho-agronomic analysis for identification of different kenaf varieties (Cheng et al, 2002). Therefore, we have used this technique for detection of genetic diversity in different kenaf genotypes originated from 15 countries (Table 2), but cultivated in single tropical environment (Malaysia).…”

“…For RAPD analysis, five OPA primer sets described by Cheng et al (2002) were used in this study. The primers were synthesized by medigene Sdn Bhd, Malaysia.…”

“…In addition, genetic variability detection using morphological traits is not worthy when the target gene expression changes with environmental condition and plant development stages (Kalpana et al, 2012). It is also important to define the circulating seeds in the market to secure farmer"s interest from potential fraudulent admixtures (Cheng et al, 2002). Traditional genetic variation analysis was on morphological and agronomical traits and due to the difficulty to identify cultivars based entirely on these traits effective recently molecular technologies are introduced (Islam et al, 2014).…”

Somaclonal variations of Soybeans (<i>Glycine Max.</i> L. Merr) stimulated by drought stress based on random amplified polymorphic DNAs (RAPDs)

“…RAPD has been reported capable of detecting the relative distance among the member of genus Hibiscus section Furcaria [20]. The genetic diversity of kenaf based on agronomic and RAPD data has been detected [21]. Characterization of flax collection by means of RAPD technique also has been done [19].…”