Comparative Studies of Chromaffin Cell Proliferation in the Adrenal Medulla of Rats and Mice

Tischler, Arthur S.; Powers, James F.; Shahsavari, Mehzad; Ziar, Jeffrey; Tsokas, Panayiotis; Downing, John C.; McClain, R.M.

doi:10.1006/faat.1996.2277

Cited by 27 publications

(7 citation statements)

References 24 publications

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“…This is especially true of the human medulla, which occupies only the head and body of the gland. 225,226 Ultrastructural examination of chromaffin cells shows large Golgi zones, small mitochondria, parallel stacks of rough endoplasmic reticulum and numerous free ribosomes and glycogen particles. 220 The principal cells of the adrenal medulla are the so-called pheochromocytes or chromaffin cells.…”

Section: Adrenal Medullamentioning

confidence: 99%

“…232 Hyperplasia of the adrenal medulla is less commonly reported in other species including humans. 226 In rodents, the principal diagnostic difficulty in the medulla is that hyperplasia forms a continuous histological spectrum with neoplastic growth (pheochromocytoma). 226 In rodents, the principal diagnostic difficulty in the medulla is that hyperplasia forms a continuous histological spectrum with neoplastic growth (pheochromocytoma).…”

Section: Hyperplasiamentioning

confidence: 99%

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Endocrine Glands

Greaves¹

2012

Histopathology of Preclinical Toxicity Studies

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Section: Adrenal Medullamentioning

confidence: 99%

Section: Hyperplasiamentioning

confidence: 99%

Endocrine Glands

Greaves¹

2012

Histopathology of Preclinical Toxicity Studies

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“…1988). Although there are interspecies differences (Tischler et al. 1997), cells from this transient structure in embryonic development (dorsal surface of the neural tube) migrate along stereotyped pathways and differentiate to form a variety of cell types, including, among others, intra (pheochromocytes) and extra‐adrenal chromaffin cells, small granule chromaffin cells, extra‐adrenal paraganglionic cells and autonomic ganglion cells (Le Douarin et al.…”

Section: Histogenesis Of Chromaffin Cellsmentioning

confidence: 99%

Histogenesis and morphofunctional characteristics of chromaffin cells

et al. 2007

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This article reviews the current status of research about the histogenesis and morphofunctional characteristics of chromaffin cells in the adrenal medulla. First, this study reports the selective migration, transcription and activation factors, and the morphological events of the chromaffin cell precursors during adrenal medulla development. Subsequently, the morphofunctional characteristics of adrenergic and non-adrenergic cells are considered, with particular reference to the characteristics of chromaffin granules and their biological steps, including their formation, traffic (storage, targeting and docking), exocytosis in the strict sense and recapture. Moreover, the relationship of chromaffin cells with other tissue components of the adrenal medulla is also revised, comprising the ganglion cells, sustentacular cells, nerves and connective-vascular tissue.

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“…Evidence to support this hypothesis is provided by studies showing that agents associated with development of pheochromocytomas in rats are mitogenic for chromaffin cell in vitro. One of these agents is the antihypertensive drug reserpine (30) which causes pheochromocytomas in rats but not in mice (35). Adrenal gland denervation inhibits the chromaffin cell proliferation induced by the administration of reserpin (30).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, novel markers such as ß III tubulin and peripherin were tested for the first time in rat adrenal medullary tumors. Because previous studies on cell proliferation in man have shown that Ki-67 is superior to PCNA (16,35), we assessed cell proliferation in rat adrenal medullary tumors using both markers in order to select a reference marker for future investigations in this field.…”

Section: Introductionmentioning

confidence: 99%

Pheochromocytomas and Ganglioneuromas in the Aging Rats: Morphological and Immunohistochemical Characterization

Pace

Perentes

Germann

2002

Toxicologic Pathology

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We investigated, morphologically and immunohistochemically, 74 medullary adrenal tumors, including 64 pheochromocytomas (14 malignant and 50 benign), 9 ganglioneuromas, and 1 malignant schwannoma. The tumors were detected in 2-year-old Wistar and Sprague-Dawley rats from carcinogenicity studies. Morphologically, benign pheochromocytomas were characterized by monomorphic, small, basophilic cells with almost absence of mitoses. Malignant pheochromocytomas presented a low grade of pleomorphism, higher rate of mitoses, necrosis, infiltrative growth and in 1 case metastases in the lung. Ganglioneuromas were characterized by ganglion and neuron-like cells embedded in an eosinophilic matrix containing neurites, Schwann cells, and scant fibrovascular elements. All pheochromocytomas were strongly immunoreactive for tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine synthesis. Subpopulations of chromaffin cells expressed chromogranin A (CGA) positivity. Matrix and Schwann cells were positive for S-100 and for glial fibrillary acidic protein (GFAP). In focal areas of the tumors, ganglion cells and axons were positive for neurofilament proteins (NFP) and synaptophysin. Ganglion cells exhibited peripherin and ß-tubulin. Proliferative activity of the tumors was assessed by immunostaining the endogenous cell proliferation associated-antigen Ki-67 and the proliferating cell nuclear antigen (PCNA). As expected, cell proliferation indeces were much higher in malignant pheochromocytomas than in benign, yet ganglioneuromas remained immunonegative. Considering that Ki-67 antigen is more specific for cell proliferation, it should be regarded as marker of choice for supporting the differential diagnosis between benign and malignant pheochromocytomas.

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Comparative Studies of Chromaffin Cell Proliferation in the Adrenal Medulla of Rats and Mice

Cited by 27 publications

References 24 publications

Endocrine Glands

Endocrine Glands

Histogenesis and morphofunctional characteristics of chromaffin cells

Pheochromocytomas and Ganglioneuromas in the Aging Rats: Morphological and Immunohistochemical Characterization

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