Comparative studies focusing on transgenic through cp4EPSPS gene and non-transgenic soybean plants: An analysis of protein species and enzymes

Integrative Modeling of Oxidative Stress and C1 Metabolism Reveals Upregulation of Formaldehyde and Downregulation of Glutathione

“…It involves successive oxidations and reductions of ascorbate, glutathione, and NADPH by the enzymes ascorbate peroxidase, glutathione reductase, dehydroascorbate reductase, and monodehydroascorbate reductase. The removal of H 2 O 2 produced in the chloroplast, as a result of the dismutation of 2 O − ⋅ , is essential to prevent inhibition of the Calvin cycle enzymes [3].…”

Evaluation of proteome alterations induced by cadmium stress in sunflower (Helianthus annuus L.) cultures

“…Proteins were extracted from the leaves of each plant group as previously described (Arruda et al, 2013;da Silva et al, 2010), with minor modifications. In brief, 1.0 g of sunflower leaves (fresh weight) was ground in a mortar in liquid nitrogen.…”

“…A comparative proteomics analysis of sunflowers leaves from the control and Cd-treated plants was performed using 2-D DIGE (Ettan DIGE, GE Healthcare, USA) as described previously (Arruda et al, 2013). 2-D DIGE gels from each group were prepared in triplicate, and two samples were evaluated by running.…”

“…The protein spots of interest identified by the DIGE study were excised manually from the Coomassie-stained gels and subjected to in-gel trypsin digestion, as described previously (Arruda et al, 2013;da Silva et al, 2010). Briefly, the spots excised from the gels were placed into a micro-SPE plate containing peptide affinity resin, and the digestion and vacuum elution protocols were performed according to the manufacturer's recommendations using the Montage s In-Gel digestZP kit (Millipore, Bedford, USA).…”

Multi-method research strategy for understanding changes in barley grain protein composition and its relation to improved nutritional quality

“…The field of proteomics has been generating a large amount of valuable information in general (BARBOSA et al, 2012;ARRUDA et al, 2013;VIDAL et al, 2015), including on cereal grain metabolism, remarkably in barley. A number of studies including protein accumulation during grain filling and maturation in the whole grain and in dissected tissues (aleurone layer, starch endosperm and embryo) (FINNIE et al, 2002;FINNIE et al, 2004b), responses to environmental and genetic fluctuations (FINNIE et al, 2004a;YANG et al, 2010;EGGERT;PAWELZIK, 2011) as well as to elucidate the relation between protein and grain quality for different end-use purposes (ØSTERGAARD et al, 2002;ØSTERGAARD et al, 2004;FINNIE et al, 2006;JIN et al, 2012) In the present study a 2-DE-based proteomic approach in combination with mass spectrometry was used to address questions on the association of non-storage proteins (albumin and globulin) and storage proteins profile front of a grain protein balance perturbation, in this case the suppression of C-hordeins in barley.…”