Metal pollution is a major environmental problem which affects agriculture
and human health. Turkey has significant Cu mining areas in Diyarbak?r and
its surrounding areas (Southeast Anatolia). Several crop plants cultivated
in these areas are irrigated with water from the Tigris, and most
agricultural lands are contaminated with Cu. Brassica nigra and B. juncea
are well-known metal accumulator plant species which can hyperaccumulate
metals, including copper, in their shoots. The purpose of this study is to
evaluate their potential for the phytoremediation of Cu from these
contaminated areas as an environmentally friendly and cost-effective means
of reducing Cu-contamination. In this research, B. nigra and B. juncea
plants were grown in soil at different Cu concentrations (0 to 1000 ?M) and
showed no toxicity symptoms while accumulating a significant amount of metal
in their leaves. In the leaves of both species, the Cu content increased
significantly with the increase in the Cu level in the media. HMA1 (Heavy
Metal ATPase 1) in the leaves of both plant species gradually increased with
increased Cu levels until 50 ?M, then its expression slowly decreased with
the further increase in Cu levels. The expression of HMA3 also increased
with an increase in Cu in the leaves of both plant species. However, its
expression pattern differed from that of HMA1. Our data showed that an
increase in Cu levels in the leaves triggers the expression of both genes,
suggesting that they play an active role in Cu detoxification. We propose
that these plant species could be used for the decontamination of Cu from
polluted soils. These data also indicate that Cu accumulation and tolerance
in both plant species is probably a multi-genetic response, possibly
involving several other transporter genes in the stress signal pathway.
Hence, we also explored the expression of the other metal transporters, such
as other HMAs (HMAs 5-8), Nramps (e.g., Nramp3), COPT proteins, and some Cu
chaperons in these plant species.