Comparative spermatozoal ultrastructure and molecular analysis in dromiid crabs and their phylogenetic implications for Dromiidae and Podotremata (Decapoda: Brachyura)

Bento, Maria Alice Garcia; Miranda, Ivana; Mantelatto, Fernando L.; Zara, Fernando José

doi:10.1016/j.asd.2018.10.001

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…The nucleus undergoes extensive morphological modifications as it changes from circular in early spermatids, to “half‐moon shaped” in intermediate spermatids, and finally, to a compact “C‐shaped” or “cup‐shaped” form that almost surrounds the entire spermatozoon. This characteristic is commonly observed in brachyurans (Jamieson, 1990; Jamieson & Tudge, 2000; Langreth, 1969; Medina, 1994; Medina & Rodríguez, 1992; Simeó et al, 2010; Tudge, 2009), and the cup‐shaped nucleus is the most common shape seen among the mature spermatozoa in this group (Jamieson, 1994), differently as observed from some Podotremata which usually has “half‐moon‐shaped” nucleus (Garcia Bento, Trentin, Mantelatto, & Zara, 2018; Jamieson, 1994).…”

Section: Discussionmentioning

confidence: 88%

“…In Callinectes , only one type of round spermatophore filled with many mature spermatozoa (coenospermic condition) is observed. This is the most common pattern seen in Eubrachyura, as opposed to the very long coenospermic spermatophore or “sperm cord” observed in Podotremata Dromiidae (Garcia Bento, López‐Greco, & Zara, 2018; Garcia Bento, Trentin, Mantelatto, & Zara, 2018) or the cleistospermic spermatophore found in freshwater crabs and in the Grapsidae Pa. gracilis (Guinot, Jamieson, & Tudge, 1997; Klaus, Schubart, & Brandis, 2009; Tiseo et al, 2014). In the cancrid crab, Can.…”

Section: Discussionmentioning

confidence: 93%

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Ultrastructure and histochemistry of the male reproductive system of the genus Callinectes Stimpson, 1860 (Brachyura: Portunidae)

Watanabe

Nascimento

Mantelatto

et al. 2020

Journal of Morphology

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We described the ultrastructure and histochemistry of the reproductive system of five Callinectes species, and evaluate the seasonal variation in weight of the reproductive system and hepatopancreas by comparing annual changes of somatic indices. The somatic indices changed little throughout the year. In Callinectes, spermatogenesis occurs inside the lobular testes and, within each lobule, the cells are at the same developmental stage. Spermatogenesis and spermiogenesis follow the same development pattern in all Callinectes studied. Mature spermatozoa are released into the seminiferous ducts through the collecting ducts. Cells of the vas deferens are secretory as evidenced by rough endoplasmic reticulum, Golgi complex, and secretory vesicles that produce the seminal fluid. The anterior vas deferens shows two portions: proximal and distal. In proximal portion (AVDp), spermatozoa are clustered and embedded in an electron-dense, basophilic glycoproteinaceous secretion Type I. In the distal portion (AVDd), the spermatophore wall is formed by incorporation of a less electron-dense glycoproteinaceous secretion Type II. The secretion Type I change to an acid polysaccharide-rich matrix that separates the spermatophores from each other. The median vas deferens (MVD) stores the spermatophores and produces the granular glycoproteinaceous seminal fluid. The posterior vas deferens (PVD) has few spermatophores. Its epithelium has many mitochondria and the PVD seminal fluid changes into a liquid and homogeneous glycoprotein. Many outpocketings in the PVD and MVD help to increase the fluid production. Overall, the reproductive pattern of Callinectes is similar to other species that produce sperm plugs. The secretions of AVD, MVD, and PVD are responsible for the polymerization that forms the solid, waxy plug in the seminal receptacle. The traits identified here are common to all Portunidae species studied so far.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 93%

Ultrastructure and histochemistry of the male reproductive system of the genus Callinectes Stimpson, 1860 (Brachyura: Portunidae)

Watanabe

Nascimento

Mantelatto

et al. 2020

Journal of Morphology

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“…However, this genus uses the sub‐chelate P4 to hold the shell while the shorter and filiform P5 only has a stabilizing or supporting function (Guinot & Tavares, 2000; McLay & Hosie, 2012). The molecular analysis shows that the apparent dromiid ancestral form is a shell‐camouflaged organism using the P5 to hold the shell as Hypoconhinae, while the shell‐carrying Conchoecetes are reminiscent of “sponge‐carrier” Dromiinae (Garcia Bento et al, 2018). Thus, the bivalve position during copulation probably is old to Dromiidae.…”

Section: Discussionmentioning

confidence: 99%

From copula to fertilization: The reproduction of primitive crabHypoconcha parasitica(Linnaeus, 1763) (Podotremata: Dromiidae)

Bento

Greco

Mantelatto

et al. 2022

The Anatomical Record

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This study describes the mating behavior of Hypoconcha parasitica under laboratory conditions highlighting the spermathecal morphology and focusing on the seminal fluid storage and release of spermatozoa. The pairs were kept in aquaria where the mating behavior was recorded and described. The spermathecae of the female were analyzed using scanning electron microscopy, X‐ray micro‐CT, histology, and histochemistry. No pre‐ or post‐copulatory mate guarding was observed in H. parasitica. The sperm transfer occurred with each pair maintaining the protection shield (bivalve shell) on their dorsum. The pair of spermathecae is covered exclusively by the cuticle, following the Podotremata pattern. Many muscle fiber bundles are attached to the cuticular wall facing toward the cephalothorax cavity, especially covering the lateral and slightly dorso‐anterior region toward the apodeme of Sternite 7. The spermathecal organization indicates that the process of sperm release during fertilization occurs through muscular action exerted by the female on the wall of the chamber. Thus, the musculature distribution in Hypoconchinae distinguishes them from described for other Podotremata such as Homolidae, which shows the musculature associated with the spermathecae aperture. Like the Homolidae, the first pleopod in H. parasitica seems to take part in the transfer/ movement of spermatozoa and oocytes between the sternum and abdomen, which forms a temporary chamber where fertilization occurs. In conclusion, the spermathecal morphology and associated structures bring new insights to the mechanisms involved in the sperm storage and fertilization of primitive crabs and how the Dromiidae spermatheca perform a new pattern among the poorly studied Podotremata.

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“…Until now, the podotreme reproductive system has been described only in terms of gross morphology and macroscopic structures, and the mechanism of fertilisation is poorly known. But new data have been recently published on the reproductive anatomy of podotremes and on the spermatozoal ultrastructure of Dromiidae leading to a molecular analysis (Garcia Bento et al 2019a, b).…”

Section: Review Of External Morphological Characters In the Seven Sub...mentioning

confidence: 99%

A new subfamily classification of the highly diversified Dorippidae H. Milne Edwards, 1837 (Crustacea, Decapoda, Brachyura, Dorippoidea), using morphological, molecular and palaeotonlogical data, with special emphasis on its unique female reproductive system

Guinot

2023

Zoosystema

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A new and revised classification of the small family Dorippidae H. Milne Edwards, 1837, which previously comprised two distinct subfamilies (Dorippinae H. Milne Edwards, 1837 and Ethusinae Guinot, 1977), each of which was subsequently elevated to a familial rank supported by traditional data and genetic phylogenies, is presented on the basis of a morphological analysis involving a large number of characters. The family is in fact highly diverse, at all levels. Several features are described and depicted in detail here for the first time, e.g. the presence of a diversely developed strip along the protrudingly rimmed posterior margin of the carapace; on the dorsally exposed thoracic sternite 8, a process acting as an additional pleonal-retention mechanism in the females of three genera (Dorippe Weber, 1795; Philippidorippe Chen, 1986; Phyllodorippe Manning & Holthuis, 1981); a callosity at the base of the coxa of the third pereiopod, variously shaped, in two genera (Dorippe and Dorippoides Serène & Romimohtarto, 1969). The generic and specific taxonomy masterfully stabilised by Holthuis & Manning (1991) is undisputed and still valid, except for the composition of the genus Heikeopsis Ng, Guinot & Davie, 2008 that may well contain in northern China a form not entirely consistent with the typical Heikeopsis japonica (von Siebold, 1824) of Japan. A second exception concerns the genus Paradorippe Serène & Romimohtarto, 1969 sensu Holthuis & Manning (1991) that may not be monophyletic. The validity of Medorippe crosnieri Chen, 1988, questioned by Holthuis & Manning (1990), could not be confirmed here: its distinctive morphological characters from M. lanata (Linnaeus, 1767) suggest that it could be recognised one day. The synthesis of conventional and new characters allows the traditional taxonomic approach to be updated and modernised, more complete and robust diagnoses to be constructed, and groups of genera to be circumscribed. We propose a new subfamily classification whereby the family Dorippidae is recognised as monophyletic but with seven genera assigned to subfamily-level rank, leading to the recognition of seven distinct subfamilies: the Dorippinae H. Milne Edwards, 1837 n. stat. (Dorippe), Dorippoidinae n. subfam. (Dorippoides), Medorippinae n. subfam. (Medorippe Manning & Holthuis, 1981), Heikeopsinae n. subfam. (Heikeopsis, Neodorippe Serène & Romimohtarto, 1969, Nobilum Serène & Romimohtarto, 1969), Paradorippinae n. subfam. (Paradorippe), Philippidorippinae n. subfam. (Philippidorippe) and Phyllodorippinae n. subfam. (Phyllodorippe). There is a high degree of concordance between several elements of our proposal: 1) the main morphological patterns of the male gonopods lead to the recognition of seven subfamilies; 2) the main morphological patterns of the vulvae correspond to the seven observed gonopod types and lead to the recognition of the same seven subfamilies; 3) the recognition of several subfamilies is supported by the well-defined clades obtained by the molecular analyses; and 4) the female re...

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Comparative spermatozoal ultrastructure and molecular analysis in dromiid crabs and their phylogenetic implications for Dromiidae and Podotremata (Decapoda: Brachyura)

Cited by 8 publications

References 31 publications

Ultrastructure and histochemistry of the male reproductive system of the genus Callinectes Stimpson, 1860 (Brachyura: Portunidae)

Ultrastructure and histochemistry of the male reproductive system of the genus Callinectes Stimpson, 1860 (Brachyura: Portunidae)

From copula to fertilization: The reproduction of primitive crabHypoconcha parasitica(Linnaeus, 1763) (Podotremata: Dromiidae)

A new subfamily classification of the highly diversified Dorippidae H. Milne Edwards, 1837 (Crustacea, Decapoda, Brachyura, Dorippoidea), using morphological, molecular and palaeotonlogical data, with special emphasis on its unique female reproductive system

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