Comparative single-cell multiplex immunophenotyping of therapy-naive patients with rheumatoid arthritis, systemic sclerosis, and systemic lupus erythematosus shed light on disease-specific composition of the peripheral immune system
József Á. Balog,
Ágnes Zvara,
Vivien Bukovinszki
et al.
Abstract:IntroductionSystemic autoimmune diseases (SADs) are a significant burden on the healthcare system. Understanding the complexity of the peripheral immunophenotype in SADs may facilitate the differential diagnosis and identification of potential therapeutic targets.MethodsSingle-cell mass cytometric immunophenotyping was performed on peripheral blood mononuclear cells (PBMCs) from healthy controls (HCs) and therapy-naive patients with rheumatoid arthritis (RA), progressive systemic sclerosis (SSc), and systemic … Show more
“…Immunophenotyping using fluorochrome- or metal-labeled antibodies has become a widely used technology to reveal the functional heterogeneity of leukocytes and to monitor protein expression detected by single-cell flow cytometry [ 1 ]. Recent progress in fluorescence flow cytometry and mass cytometry has enabled high-dimensional resolution of the complex immunophenotype in both autoimmune diseases and cancer [ 2 , 3 , 4 ]. Therefore, we invited authors to publish their latest achievements related to the perturbation of the regulation of immune activation and the discovery of rare subpopulations of innate and adaptive immune players in autoimmune diseases or cancer.…”
“…Immunophenotyping using fluorochrome- or metal-labeled antibodies has become a widely used technology to reveal the functional heterogeneity of leukocytes and to monitor protein expression detected by single-cell flow cytometry [ 1 ]. Recent progress in fluorescence flow cytometry and mass cytometry has enabled high-dimensional resolution of the complex immunophenotype in both autoimmune diseases and cancer [ 2 , 3 , 4 ]. Therefore, we invited authors to publish their latest achievements related to the perturbation of the regulation of immune activation and the discovery of rare subpopulations of innate and adaptive immune players in autoimmune diseases or cancer.…”
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