Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults

Abstract

Steroid-induced osteonecrosis of the femoral head (SONFH) is a disabling, aseptic and ischemic disease that develops following steroid therapy. The pathogenesis of SONFH is unclear, so the early diagnosis and treatment for this disease is yet to be established. The purpose of the present study was to identify potential biomarkers for SONFH. The differential expression of serum proteins from patients with SONFH and healthy volunteers was analyzed by the proteomics method. The protein samples were labeled and subjected to isoelectric focusing and two-dimensional gel electrophoresis. The resultant protein spots were matched and quantified by an imaging analysis system. The differentially-expressed protein spots were subjected to in-gel trypsin digestion followed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Significantly lower levels of complement component 3 (C3), C4, inter-α-trypsin inhibitor heavy chain H4 and α-2-macroglobulin were found in the serum of patients with SONFH. These proteins are reported to be actively involved in intravascular coagulation, apoptosis and reactive oxygen species imbalance, indicating that multiple pathological reactions occur in SONFH and these proteins may serve as potential biomarkers for the diagnosis of SONFH.

Serum Amyloid A Was Correlated with Osteonecrosis of the Femoral Head by Affecting Bone Metabolism

“…Although MRI has made a massive contribution to the early diagnosis of ONFH, the failure to evaluate the activity status and prognosis of the disease results in that numbers of patients miss the opportunities for timely treatment. The proteomics technology has been used to analyze the sera proteins levels which altered in ONFH patients, of which the aim is to explore the biomarkers of ONFH [35]. Previous studies have established the relationship between matrix protein, angiogenic molecule and the occurrence of ONFH in experimental animal models [36,37].…”

Relationship of α2‐Macroglobulin with Steroid‐Induced Femoral Head Necrosis: A Chinese Population‐Based Association Study in Southeast China

“…α2‐Macroglobulin is indicated to significantly increase the regenerative capacity of plasma plasmin, while the complex formed by the binding of α2‐macroglobulin and vascular endothelial growth factor inhibits the activity of heparin, resulting in a hypercoagulable state of the blood. Chen et al . confirmed that the level of α2‐macroglobulin in bone tissue of patients with steroid‐induced femoral head necrosis was significantly decreased, which may be related to the hypercoagulability of the blood, hyperlipidemia, free radicals, and degradation of matrix metalloproteinase.…”

Serum biomarkers discovery of steroid-induced femoral head osteonecrosis in Chinese female by comparative proteome

“…In the last few years, SONFH has attracted extensive attention, various hypotheses have been put forward for the etiological factors involved in corticosteroid-induced osteonecrosis, and it is now considered multiple factors contribute to the development of SONFH, including blood coagulation disorders, abnormal lipid metabolisms [7,8], disorders of the vascular endothelium [9], fat embolism [10], oxidative stress [11][12][13], apoptosis [8], genetic variants, thrombosis, alcohol abuse, long-term corticosteroid use [14], systemic lupus erythematosus, inflammatory bowel disease, and also for immunosuppression after renal transplants [15][16][17]. Though there are many clinical and basis studies about femoral head necrosis in previous studies, it is still unable to clarify the specific pathophysiological mechanism of the process by which corticosteroid administration induces the development of osteonecrosis [18,19].…”

“…The beginning of proteomic technology applying and screen biomarker in femoral head necrosis is relatively late and there are rare reports [12,13,21,22]. So, in this study, differentially expressed protein in serum among SONFH, TONFH patients and healthy volunteers in Chinese females was compared using iTRAQ, the proteomics study of femoral head necrosis will be helpful to screen potential diagnostic biomarkers for SONFH.…”