Comparative sensitivities to antimicrobial agents of Campylobacter pylori and the gastric campylobacter like organism from the ferret.

Abstract

1265row showed erythrobastopenia (less than 1% of erythroblasts). The presence of serum anti-HPV IgM (radioimmunoassay) suggested a recent HPV infection. Packed red cells (600 ml) were transfused. Over the next few days the symptoms disappeared and haemoglobin concentrations remained stable. Ten days after admission reticulocyte count was 150 x 109/1. Eighteen months later, the patient was quite well and all haematological investigations yielded normal results blood count, haemoglobin electrophoresis, erythrocytic enzymes (glucose-6-phosphate-deshydrogenase, glucose-phosphogluconate-deshydrogenase, hexokinase, glucose-isomerase-phosphate, glucose-pyruvate, glutathione reductase, acetyl-cholinesterase, pyrimidine-5'-nucleotidase), osmotic resistance and autohaemolysis.Our observation of HPV infection associated with aplastic crisis but without haemolysis differs from the transient erythroblastopenia seen in childhood, which often affects younger children (1 to 4 years) and occurs without HPV infection.3Aplastic crisis associated with HPV infection has hitherto only been described in hereditary2 4-6 or acquired' haemolytic anaemias. It seems that the erythroblastopenic effect of HPV is constant but goes unnoticed if the red cell life span is normal. A shortened red cell survival (haemolysis) is necessary to cause acute anaemia. Acute anaemia occurring without haemolysis due to an HPV infection is difficult to explain. In our patient only isotopic labelling of his erythrocytes could have completely excluded underlying haemolysis. Nonetheless, we wanted to record the experience to encourage doctors to ask for parvovirus serology in similar clinical circumstances.

Bactericidal effect of amoxicillin on Helicobacter pylori in an in vitro model using epithelial cells

“…Amoxicillin treatment has given the best results, with approximately 10 to 20% eradication (3). These results contrast with the apparently high susceptibility of H. pylori in vitro to most antimicrobial agents, including amoxicillin (8,10,13,15,20), which indicate that the traditional MIC determination is not adequate for testing this mucosa-associated bacterium.The concept of biofilm has been proposed by Costerton (6). By studying industrial ecosystems, Costerton (6) showed that attached organisms (sessile bacteria) differ markedly from floating organisms (planktonic bacteria) that are present in the same ecosystem and differ even more markedly from cells that are grown in pure culture in laboratory media.…”

“…Amoxicillin treatment has given the best results, with approximately 10 to 20% eradication (3). These results contrast with the apparently high susceptibility of H. pylori in vitro to most antimicrobial agents, including amoxicillin (8,10,13,15,20), which indicate that the traditional MIC determination is not adequate for testing this mucosa-associated bacterium.…”

Molecular cloning and expression of Campylobacter pylori species-specific antigens in Escherichia coli K-12

“…Antiserum to the cloned antigens was raised in a rabbit by lysing E. coli K-12 strain 392 with the recombinant phage XCP2 in liquid culture (24) b Strains isolated from human antral mucosa biopsy specimens originally described as GCLO type 11 (20). c Campylobacterlike organisms isolated from the gastric mucosa of a pig (P1), baboon (B3), and ferrets (F6, W0831, and W0834) (11,15).…”

Emergence of DiverseHelicobacterSpecies in the Pathogenesis of Gastric and Enterohepatic Diseases

“…Limited studies of antibiotic therapy for H. mustelae indicate that it is sensitive in vitro to many of the agents effective against H. pylori. The MIC of amoxicillin is 1 to 2 log units higher than for H. pylori (4,221,313), and therefore combination treatment containing amoxicillin may be less effective in ferrets than in humans (4,313). The combination of ranitidine bismuth citrate and clarithromycin is effective therapy for H. mustelae infection in ferrets (263).…”