Comparative secretome analysis between salinity-tolerant and control Chlamydomonas reinhardtii strains

Ves-Urai, Parthompong; Krobthong, Sucheewin; Thongsuk, Karnpitcha; Roytrakul, Sittiruk; Yokthongwattana, Chotika

doi:10.1007/s00425-021-03583-7

Cited by 6 publications

(6 citation statements)

References 96 publications

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“…2014) stresses. Furthermore, a recent analysis in Chlamydomonas comparing an evolved salinity-tolerant strain to the parental strain found over 500 differentially accumulated proteins between the two secretomes (Ves-Urai, Krobthong, Thongsuk, Roytrakul & Yokthongwattana 2021). Recently, a high temperature secretome of Sorghum bicolor cell cultures identified 31 heat-responsive secreted proteins functioning in protein modification, detoxification, and metabolism (Ngcala, Goche, Brown, Chivasa & Ngara 2020).…”

Section: Discussionmentioning

confidence: 99%

High-throughput Identification of Novel Heat Tolerance Genes via Genome-wide Pooled Mutant Screens in the Model Green AlgaChlamydomonas reinhardtii

Mattoon

McHargue

Bailey

et al. 2022

Preprint

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High temperatures adversely affect crop and algal yields. Photosynthetic cells show varying physiological responses to different heat intensities. The specificity of genes required for thermotolerance at different temperatures in photosynthetic cells remains elusive. Additionally, organic carbon sources are present in nature for both plants and algae, but it is unclear how environmental availability of carbon sources interface with heat responses. We utilized the insertional mutant library of the unicellular, eukaryotic green alga Chlamydomonas reinhardtii (CLiP) to perform genome-wide, quantitative, pooled screens under moderate (35oC) or acute (40oC) high temperatures with or without organic carbon. We screened for heat sensitive mutants based on growth rate quantification and identified putative heat tolerance genes (HTGs). By triangulating HTGs with heat-induced transcripts/proteins in wildtype cultures and MapMan functional annotation data, we present a high-confidence list of 933 Chlamydomonas genes with putative roles in heat tolerance. These include genes with known roles in thermotolerance and those with little or no functional annotation, suggesting novel players in thermotolerance. About 50% of these high-confidence HTGs in Chlamydomonas have orthologs in green lineage model organisms, including crop species. Arabidopsis thaliana mutants deficient in the ortholog of a Chlamydomonas HTG were also heat sensitive. This work expands our knowledge of heat responses in photosynthetic cells and provides engineering targets to improve thermotolerance in algae and crops.

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Section: Discussionmentioning

confidence: 99%

High-throughput Identification of Novel Heat Tolerance Genes via Genome-wide Pooled Mutant Screens in the Model Green AlgaChlamydomonas reinhardtii

Mattoon

McHargue

Bailey

et al. 2022

Preprint

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“…This review also points out that, besides proteomic and transcriptomic studies, very few data are available on osmosensors and on the signaling networks/upstream components mediating salt stress responses, although some more specific studies are emerging. Moreover, some information on how cells might communicate with each other to induce an early stress response may offer interesting insights for future studies [92].…”

Section: Discussionmentioning

confidence: 99%

“…Despite the visible acclimation of the population to the high salt conditions, several proteins involved in stress and defense (i.e., ROS detoxifying enzymes) were still significantly up-regulated in the salt-tolerant population, as well as several kinases, membrane transport proteins, and proteolytic enzymes for protein turnover and amino acid recycling. Interestingly, under these conditions, the extracellular secreted proteins also differed between the salinity-tolerant and the control strain, with 203 up-regulated and 110 down-regulated proteins in the salinity-tolerant strain [92]. Many signaling proteins, channels, and membrane transport and trafficking proteins were up-regulated in the salinity-tolerant secretome, such as CaM, 1 4-3-3 homologs, matrix metalloproteases, and Fructose-1,6-bisphosphate aldolase (FBA).…”

Section: Omics Studiesmentioning

confidence: 99%

“…Many signaling proteins, channels, and membrane transport and trafficking proteins were up-regulated in the salinity-tolerant secretome, such as CaM, 1 4-3-3 homologs, matrix metalloproteases, and Fructose-1,6-bisphosphate aldolase (FBA). Moreover, many proteins lacked a signal peptide, suggesting the existence of an unconventional protein secretion pathway in Chlamydomonas, which might facilitate a rapid release of the stress-related proteins [92]. Therefore, saline stress acts also on the secreted proteins and likely communication among cells is involved in the stress response.…”

Section: Omics Studiesmentioning

confidence: 99%

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Chlamydomonas Responses to Salinity Stress and Possible Biotechnological Exploitation

Bazzani

Lauritano

Mangoni

et al. 2021

JMSE

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Salinity is among the main drivers affecting growth and distribution of photosynthetic organisms as Chlamydomonas spp. These species can live in multiple environments, including polar regions, and have been frequently studied for their adaptation to live at different salinity gradients. Upon salinity stress (hypersalinity is the most studied), Chlamydomonas spp. were found to alter their metabolism, reduce biomass production (growth), chlorophyll content, photosynthetic activity, and simultaneously increasing radical oxygen species production as well as lipid and carotenoid contents. This review summarizes the current literature on salt stress related studies on the green algae from the genus Chlamydomonas considering physiological and molecular aspects. The overall picture emerging from the data suggests the existence of common features of the genus in response to salinity stress, as well as some differences peculiar to single Chlamydomonas species. These differences were probably linked to the different morphological characteristics of the studied algae (e.g., with or without cell wall) or different sampling locations and adaptations. On the other hand, molecular data suggest the presence of common reactions, key genes, and metabolic pathways that can be used as biomarkers of salt stress in Chlamydomonas spp., with implications for future physiological and biotechnological studies on microalgae and plants.

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“…Furthermore, a recent analysis in Chlamydomonas comparing an evolved salinity-tolerant strain to the parental strain found over 500 differentially accumulated proteins between the two secretomes (Ves-Urai et al, 2021). Recently, a high-temperature secretome of Sorghum bicolor cell cultures identified 31 heat-responsive secreted proteins functioning in protein modification, detoxification and metabolism (Ngcala et al, 2020).…”

Section: Htgs Were Overrepresented In the Secretory Pathwaymentioning

confidence: 99%

High‐throughput identification of novel heat tolerance genes via genome‐wide pooled mutant screens in the model green alga Chlamydomonas reinhardtii

Mattoon

McHargue

Bailey

et al. 2022

Plant Cell & Environment

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Different high temperatures adversely affect crop and algal yields with various responses in photosynthetic cells. The list of genes required for thermotolerance remains elusive. Additionally, it is unclear how carbon source availability affects heat responses in plants and algae. We utilized the insertional, indexed, genome‐saturating mutant library of the unicellular, eukaryotic green alga Chlamydomonas reinhardtii to perform genome‐wide, quantitative, pooled screens under moderate (35°C) or acute (40°C) high temperatures with or without organic carbon sources. We identified heat‐sensitive mutants based on quantitative growth rates and identified putative heat tolerance genes (HTGs). By triangulating HTGs with heat‐induced transcripts or proteins in wildtype cultures and MapMan functional annotations, we presented a high/medium‐confidence list of 933 Chlamydomonas genes with putative roles in heat tolerance. Triangulated HTGs include those with known thermotolerance roles and novel genes with little or no functional annotation. About 50% of these high‐confidence HTGs in Chlamydomonas have orthologs in green lineage organisms, including crop species. Arabidopsis thaliana mutants deficient in the ortholog of a high‐confidence Chlamydomonas HTG were also heat sensitive. This work expands our knowledge of heat responses in photosynthetic cells and provides engineering targets to improve thermotolerance in algae and crops.

show abstract

Comparative secretome analysis between salinity-tolerant and control Chlamydomonas reinhardtii strains

Cited by 6 publications

References 96 publications

High-throughput Identification of Novel Heat Tolerance Genes via Genome-wide Pooled Mutant Screens in the Model Green AlgaChlamydomonas reinhardtii

High-throughput Identification of Novel Heat Tolerance Genes via Genome-wide Pooled Mutant Screens in the Model Green AlgaChlamydomonas reinhardtii

Chlamydomonas Responses to Salinity Stress and Possible Biotechnological Exploitation

High‐throughput identification of novel heat tolerance genes via genome‐wide pooled mutant screens in the model green alga Chlamydomonas reinhardtii

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