Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains

Abstract

Background: Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic biomass. The commercial enzyme mixtures produced at industrial scales are not well characterized, and their proteinaceous components are poorly identified and quantified. The development of proteomic methods has made it possible to comprehensively overview the enzymes involved in lignocellulosic biomass degradation which are secreted under various environmental conditions.

Evolution and impact of cellulose architecture during enzymatic hydrolysis by fungal cellulases

“…The production followed the procedure previously described by Herpoel-Gimbert [44]. Commercial β-glucosidase preparation was purchased from Novozymes (SP188; Novo Nordisk A/S, Bagsvaerd, Denmark).…”

First Structural Insights into α-l-Arabinofuranosidases from the Two GH62 Glycoside Hydrolase Subfamilies

“…The amplified fragment was subcloned into the pCRII-TOPO vector (Invitrogen) and subjected to sequencing to check the integrity of the sequence. The full-length ORF corresponding to UmAbf62A was inserted at the corresponding sites (EcoRI and XbaI) into the pPICZ␣A vector in-frame with both the yeast ␣-secretion factor and C-term-(His) 6 tag encoding sequences. The resulting recombinant expression plasmid was transformed into Pichia pastoris X33 and the best-producing transformant was selected for recombinant UmAbf62A production and purification as previously described (18).…”

“…Among the hemicellulases that depolymerize arabinoxylans, ␣-L-arabinofuranosidases (EC 3.2.1.55) are exoacting enzymes that remove L-arabinofuranosyl side chain moieties from the main chain xylan, thus enhancing the action of other enzymes, such as ␤-D-xylosidase and ␤-D-xylanases (4). ␣-L-Arabinofuranosidases are mainly grouped in glycoside hydrolase (GH) 6 families GH43, GH51, and GH62 of the CAZy classification (5). Among these families, the GH62 family is the smallest with 121 members (as of 12th December 2013) and has so far received little attention.…”

Comparison of humic acids production by Trichoderma viride and Trichoderma reesei using the submerged fermentation of oil palm empty fruit bunch

“…With respect to the highest HA production for each species (Trial 2 for T. reesei and Trial 5 for T. viride), this value increases to 3-fold. Although many species of Trichoderma have previously been used for industrial enzyme production and lignocellulosic degradation (Nevalainen et al, 1994), T. reesei is known to be the main producer of cellulases and hemicellulases acting in synergy to degrade lignocellulosic materials (Herpoël-Gimbert et al, 2008), which explains its increased production of HA. To understand the effect of the variables involved in the proposed process, PB statistical analysis was used, in which the response variable (HA concentration) was examined at 120 h, because HA accumulated in the culture medium, due to the resistance of HA to microbial degradation compared to other organic materials (Aiken, 1985).…”

“…Trichoderma species produces large quantities of hydrolytic enzymes (Papavizas, 1985) including chitinases, β-1,3-glucanases (Howell et al, 2000), cellulases, amylases and proteases (Bastos, 1996), which explains the high growth capability of each species. However, T. reesei is regarded in the literature as one of the main producers of endoglucanases, secreting at least five types of these enzymes and justifying the increased growth in culture compared to T. viride, because the oat media is comprised of β-glucans that can be hydrolyzed to glucose, providing the fungus with a readily usable carbon source for growth (Herpoël-Gimbert et al, 2008).…”