Comparative Root Protein Profiles of Korean Ginseng (<i>Panax ginseng</i>) and Indian Ginseng (<i>Withania somnifera</i>)

Nagappan, Arulkumar; Karunanithi, Nithya; Sentrayaperumal, Sundareswaran; Park, Kwang-II; Park, Hyeonsoo; Lee, Dong Hoon; Kang, Suk-Yun; Kim, Jina; Senthil, Kalaiselvi; Senthil, N.; Raveendran, M.; Kim, Gon Sup

doi:10.1142/s0192415x12500164

Cited by 20 publications

(10 citation statements)

References 48 publications

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“…150.3, the protein patterns of three extraction methods seemed similar with the proteins ranging in size from 10 to 150 kDa. The total number of protein spots on 2-DE gels of method A was 389 ± 16, which were increased more than 2.5 fold compared with the results reported [16,[24][25][26], and method B and method C were only 294 ± 13 and 345 ± 16 respectively. Relative to other extraction methods, the area in size from 25 to 75 kDa of method a, had less horizontal streaking and more protein spots (as shown in the box of Fig.…”

Section: Comparison Of 2-de Profiles Of Different Lysis Buffers and Ementioning

confidence: 58%

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An Extraction Method Suitable for Two-Dimensional Electrophoresis of Low-abundant Proteins from Ginseng Roots

Jiang

Sun

et al. 2013

Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

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Four lysis buffers, which contain different concentration ratio of urea and thiourea, and three extraction methods (sonication extraction, osmotic extraction, and freeze-thaw extraction) were used to optimize the extraction conditions of low-abundant proteins from ginseng roots based on protein extraction efficiency, the good quality of the 2-DE patterns, and the results of mass spectrometry (MS) identification. Our results have shown that when lysis buffer II (7 M urea, 2 M thiourea, 2 % CHAPS, 1 % PMSF, and 1 % protease inhibitor) combined with sonication was used, higher protein extraction efficiency, lower background, more protein spots (987 ± 31), and higher spot intensities of lowabundant proteins were obtained, which indicated that the optimized protocol was suitable for proteomic studies of ginseng root. Moreover, those results provided the foundation for further researches on the significant functional proteins of ginseng roots.

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Section: Comparison Of 2-de Profiles Of Different Lysis Buffers and Ementioning

confidence: 58%

“…150.4). The overall quality of the protein profiles were good, with less vertical and horizontal streaking and smearing, and the number of protein spots was 987 ± 33, being far above the results reported [16,[24][25][26]. The optimized protocol can also be suitable for 2-DE of 24 cm strips.…”

Section: -De By the Optimized Protocolmentioning

confidence: 67%

An Extraction Method Suitable for Two-Dimensional Electrophoresis of Low-abundant Proteins from Ginseng Roots

Jiang

Sun

et al. 2013

Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

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“…These phytochemicals possess antioxidant activities that can be used for the treatment of multiple ailments (Sharma et al, 2011;Nagappan et al, 2012). WS reduces the levels of acetylcholine and catecholamine, and increases levels of serotonin and histamine in the brain (Jain et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Potentiation of NMDA Receptors byWithania somniferaon Hippocampal CA1 Pyramidal Neurons

2013

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In Ayurveda,Withania somnifera (WS) is used as a medicine to maintain mental and physical health as well as to enhance memory. In this study, the methanolic extract of WS(mWS) was tested for its electrical influence on hippocampal CA1 pyramidal neurons using a patch clamp technique. In current clamp mode under a high chloride pipette solution, mWS (400 ng/μl) induced remarkable membrane depolarization (9.75 ± 2.54 mV, n = 6) of CA1 neurons. The mWS-induced depolarization was dose-dependent, reproducible, and persistent in the presence of 0.5 μM tetrodotoxin (TTX, 10.17 ± 0.04 mV, n = 6). In voltage clamp mode (holding potential = -60 mV), mWS induced a dose-dependent non-desensitizing inward current that persisted in the presence of TTX (0.5 μM), suggesting that the response induced by mWS was purely a postsynaptic event. Interestingly, these inward currents were partially blocked by strychnine, a glycine receptor blocker. Further, mWS potentiated the NMDA response in hippocampal CA1 neurons at low concentrations. Overall, these results suggest that there are compounds in WS with possible glycine mimetic activities, which may be potential targets for inducing memory consolidation in hippocampal CA1 neurons.

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“…The 2 DE patterns of the main root and leaves of Panax ginseng was different from that of the cultured hairy root [10]. The 2 DE patterns of the Korean Ginseng (Panax ginseng C. A. Meyer) and Indian Ginseng (Withania somnifera) were also distinct [11]. The proteome of Gynura procumbens (Lour.)…”

Section: Introductionmentioning

confidence: 93%

Variations of metabolites and proteome in Lonicera japonica Thunb. buds and flowers under UV radiation

Zhu

Zheng

et al. 2017

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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Lonicera japonica Thunb., also known as Jin Yin Hua and Japanese honeysuckle, is used as a herbal medicine in Asian countries. Its flowers have been used in folk medicine in the clinic and in making food or healthy beverages for over 1500years in China. To investigate the molecular processes involved in L. japonica development from buds to flowers exposed to UV radiation, a comparative proteomics analysis was performed. Fifty-four proteins were identified as differentially expressed, including 42 that had increased expression and 12 that had decreased expression. The levels of the proteins related to glycolysis, TCA/organic acid transformation, major carbohydrate metabolism, oxidative pentose phosphate, stress, secondary metabolism, hormone, and mitochondrial electron transport were increased during flower opening process after exposure to UV radiation. Six metabolites in L. japonica buds and flowers were identified and relatively quantified using LC-MS/MS. The antioxidant activity was performed using a 1,1-diphenyl-2-picrylhydrazyl assay, which revealed that L. japonica buds had more activity than the UV irradiated flowers. This suggests that UV-B radiation induces production of endogenous ethylene in L. japonica buds, thus facilitating blossoming of the buds and activating the antioxidant system. Additionally, the higher metabolite contents and antioxidant properties of L. japonica buds indicate that the L. japonica bud stage may be a more optimal time to harvest than the flower stage when using for medicinal properties.

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Comparative Root Protein Profiles of Korean Ginseng (Panax ginseng) and Indian Ginseng (Withania somnifera)

Cited by 20 publications

References 48 publications

An Extraction Method Suitable for Two-Dimensional Electrophoresis of Low-abundant Proteins from Ginseng Roots

An Extraction Method Suitable for Two-Dimensional Electrophoresis of Low-abundant Proteins from Ginseng Roots

Potentiation of NMDA Receptors byWithania somniferaon Hippocampal CA1 Pyramidal Neurons

Variations of metabolites and proteome in Lonicera japonica Thunb. buds and flowers under UV radiation

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