Comparative reproductive biology of deep-sea ophiuroids inhabiting polymetallic-nodule fields in the Clarion-Clipperton Fracture Zone

Abstract

Deep-sea mining in the NE Pacific Clarion-Clipperton Fracture Zone (CCZ), a low-energy sedimentary habitat with polymetallic nodules, is expected to have considerable and long-lasting environmental impact. Recent studies have revealed extraordinarily high species diversity in the CCZ, in nearly all domains of Life present. Data on species biology and ecology remain scarce however. The current study describes the reproductive biology of Ophiosphalma glabrum (Lütken & Mortensen, 1899) (Ophiosphalmidae) and Ophiacantha cosmica (Lyman, 1878) (Ophiacanthidae), two ophiuroids frequently found in the CCZ. Specimens collected in Spring 2015 and 2019 in four contract areas (BGR, IOM, GSR, Ifremer) and one Area of Particular Interest (APEI 3) were examined morphologically and histologically. Size-class frequencies (disc diameter and oocytes feret diameters), sex ratios, gametogenic status, putative reproductive mode and a simple proxy for fecundity are presented. Habitat use differs in each. While O. glabrum is epibenthic, occurring as single individuals, O. cosmica often form size-stratified groups living on stalked sponges, suggesting gregarious settlement or retention of offspring (though no brooding individuals were found). Further molecular analyses are needed to establish whether O. cosmica groups are familial. In O. glabrum, for which sample sizes were larger, sex ratios approximated a 1:1 ratio with no size-structuring. In both species, individuals were at various stages of gametogenic maturity but no ripe females were identified. Based on this, O. glabrum is most probably gonochoric. Reproductive mode remains inconclusive for O. cosmica. Both species are presumptively lecithotrophic, with vitellogenic-oocyte feret diameters exceeding 250 μm. Oocyte feret diameters at times exceeded 400 μm in O. glabrum, indicating substantial yolk reserves. Estimates of instantaneous fecundity (vitellogenic specimens of O. glabrum only) were confounded by interindividual variability in gonad characteristics. Well-furnished lecithotrophic larvae, like those of O. glabrum, likely enable extended periods of larval transport, under favourable environmental conditions. The current study examines ophiuroid reproductive biology over multiple localities in the CCZ concurrently for the first time, at sites characterised by differing productivity regimes. The reproductive biology of each species is thus discussed with regard to past evolutionary (habitat stability), contemporary (food supply) and future environmental drivers (potential impacts of deep-sea mining).

Ophiotholia (Echinodermata: Ophiuroidea): A little-known deep-sea genus present in polymetallic nodule fields with the description of a new species

“…Species of the class Ophiuroidea represent all kinds of feeding types and show different lifestyles (e.g. Pearson and Gage, 1984;O'Hara et al, 2008;Mosher and Watling, 2009;Stöhr et al, 2012;Laming et al, 2021;Manjoń-Cabeza et al, 2021). So, it is incorrect to generalize the behavior of the whole class.…”

“…Among taxa collected in the CCZ, the class Ophiuroidea (Echinodermata) are widespread and abundant (Simon-Lledóet al, 2019b;Christodoulou et al, 2020). Ophiuroids inhabit all kind of habitats, from the tropics to the poles and from shallow waters to deep-sea ecosystems (O'Hara, 2007;Stöhr et al, 2012;Bribiesca-Contreras et al, 2019;Laming et al, 2021). Because of their global distribution, ophiuroids serve as excellent model organisms for the understanding of large-scale diversity and evolution patterns (Hunter and Halanych, 2008;Cho and Shank, 2010;Galaska et al, 2017;Galaska et al, 2019;Jossart et al, 2019;O'Hara et al, 2019;Stöhr et al, 2020;Eichsteller et al, 2022).…”