Comparative removal of two antibiotic resistant bacteria and genes by the simultaneous use of chlorine and UV irradiation (UV/chlorine): Influence of free radicals on gene degradation

Phattarapattamawong, Songkeart; Chareewan, Narissara; Polprasert, Chongrak

doi:10.1016/j.scitotenv.2020.142696

Cited by 52 publications

(21 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HO • has been demonstrated to play an important role in the inactivation of tetracycline-resistant Escherichia coli and in the removal of antibiotic resistance genes (ARGs) ( e.g. , sul 1 and blt ) by UV/chlorine treatment. − Additionally, HO • promotes the elimination of extracellular deoxyribonucleic acids (eDNA) in the UV/H 2 O 2 process and in the natural organic matter photosensitized system. , However, HO • is nonselective and results in rapid consumption by cellular components before contacting intracellular DNA (iDNA). , In contrast, RCS (Cl • , Cl 2 •– , and ClO • ) selectively react with compounds containing electron-rich groups, such as amino acids, pyrimidines, and purines. In addition, the concentrations of RCS, especially ClO • , are higher than that of HO • in the UV/chlorine process .…”

Section: Introductionmentioning

confidence: 99%

“…Liu et al. (2020) and Phattarapattamawong et al (2021) reported that HO • plays a primary role in the degradation of tet M, bla TEM , and sul 1 genes, while RCS play minor roles. However, Zhang et al (2019) reported that RCS play a critical role in the degradation of sul 1 and intI 1 genes in the UV/chlorine process at a chlorine dosage of 20 mg/L . Thus, the roles of RCS in UV/chlorine disinfection need further investigation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of the UV/Chlorine Process in the Disinfection of Pseudomonas aeruginosa: Efficiency and Mechanism

Wang

Guo

et al. 2021

Environ. Sci. Technol.

137

View full text Add to dashboard Cite

UV irradiation and chlorination have been widely used for water disinfection. However, there are some limitations, such as the risk of generating viable but nonculturable bacteria and bacteria reactivation when using UV irradiation or chlorination alone. This study comprehensively evaluated the feasibility of the UV/chlorine process in drinking water disinfection, and Pseudomonas aeruginosa was selected as the target microorganism. The number of culturable cells was effectively reduced by more than 5 orders of magnitude (5-log 10 ) after UV, chlorine, and UV/chlorine treatments. However, intact and VBNC cells were detected at 10 3 to 10 4 cells/mL after UV and chlorine treatments, whereas they were undetectable after UV/chlorine treatment due to the primary contribution of reactive chlorine species (Cl • , Cl 2•− , and ClO • ). After UV/chlorine treatment, the metabolic activity determined using single cell Raman spectroscopy was much lower than that after UV. The level of toxic opr gene in P. aeruginosa decreased by more than 99% after UV/chlorine treatment. Importantly, bacterial dark reactivation was completely suppressed by UV/chlorine treatment but not UV or chlorination. This study suggests that the UV/chlorine treatment can completely damage bacteria and is promising for pathogen inactivation to overcome the limitations of UV and chlorine treatments alone.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of the UV/Chlorine Process in the Disinfection of Pseudomonas aeruginosa: Efficiency and Mechanism

Wang

Guo

et al. 2021

Environ. Sci. Technol.

137

View full text Add to dashboard Cite

show abstract

“…The UV-C/FC process was more effective than UV-C and chlorination as a standalone process in the removal of tet M and bla Tem genes from aqueous solutions. 132 More specifically, the UV-C/FC process (9.03 mW cm −2 ) reduced the tet M and bla Tem genes by 0.98–3.20 log and 1.28–3.36 log, respectively, as the chlorine dose was increased from 0.5 to 20.0 mg L −1 . However, when more realistic conditions were investigated (real secondary-treated wastewater and 0.91 mW cm −2 UV-C dose), the target ARGs (belonging to phenicol, tetracycline, macrolide, fluoroquinolone, oxazolidinone, and penam classes of antibiotics) were found to be enriched by all the three disinfection treatments (UV-C, chlorination, and UV-C/FC), the most prominent increase being observed for UV-C/FC.…”

Section: Tertiary Treatment Of Urban Wastewater By Hp-aopsmentioning

confidence: 98%

Addressing main challenges in the tertiary treatment of urban wastewater: are homogeneous photodriven AOPs the answer?

Rizzo

2022

Environ. Sci.: Water Res. Technol.

View full text Add to dashboard Cite

show abstract

“…However, the specific roles of radicals in UV/chlorine for disinfection are debated. Some studies reported that HO· plays the predominant role in the inactivation of bacteriophage MS2 and the degradation of ARGs ( tet M and bla Tem) in Escherichia coli , while RCS play minor roles. , The others reported that RCS play obvious roles in the removal of ARGs ( sul 1) in Pseudomonas HLS-6 and the inactivation of Pseudomonas aeruginosa . , Currently, the studies on UV/chlorine disinfection are very limited. The roles and mechanisms of RCS for the abatement of microbial contaminants in the UV/chlorine process are still largely unknown, such as the attacking sites and specific contributions, which need further study.…”

Section: Abatement Of Contaminants By the Uv/chlorine Processmentioning

confidence: 99%

UV/Chlorine Process: An Efficient Advanced Oxidation Process with Multiple Radicals and Functions in Water Treatment

et al. 2022

View full text Add to dashboard Cite

Metrics & More Article Recommendations * sı Supporting Information CONSPECTUS: Because of the deterioration of global water quality, the occurrence of chemical and microbial contaminants in water raises serious concerns for the health of the population. Identifying and developing effective and environmentally friendly water treatment technologies are critical to obtain clean water. Among the various technologies for the purification of water, ultraviolet photolysis of chlorine (UV/chlorine), an emerging advanced oxidation process (AOP), has multiple functions for the control of contaminants via the production of hydroxyl radicals (HO•) and reactive chlorine species (RCS), such as Cl•, ClO•, and Cl 2 • − . This Account centers around the radical chemistry of RCS and HO• in different water matrices and their roles and mechanisms in the abatement of contaminants. The concentrations of Cl•, ClO•, and Cl 2 • − are comparable to or higher than those of HO• (10 −14 to 10 −13 M). The reactivities of RCS are more selective than HO• with a broader range of second-order rate constants (k). The k values of Cl• toward most aromatics are higher or similar as compared to those of HO•, while those of Cl 2 • − and ClO• are less reactive but more selective toward aromatics containing electron-donating functional groups. Their major reaction mechanisms with Cl• are electron transfer and addition, while those with ClO• and Cl 2 • − primarily involve electron transfer. As for aliphatics, their reactivities with both HO• and RCS are much lower than those of aromatics. The reaction mechanisms for most of them with Cl• and Cl 2 • − are hydrogen abstraction, except for olefins, which are addition. In addition, RCS greatly contribute to the inactivation of microbial contaminants.Toward future application, the UV/chlorine process has both pros and cons. Compared with the traditional HO•-based AOP of UV/ H 2 O 2 , UV/chlorine is more efficient and energy-saving for oxidation and disinfection, and its efficiency is less affected by water matrix components. However, the formation of toxic byproducts in UV/chlorine limits its application scenarios. In dissolved organic matter (DOM)-rich water, the formation of halogenated byproducts is enhanced in UV/chlorine. In the presence of ammonia, reactive nitrogen species (RNS) (e.g., •NO and •NO 2 ) are involved, and highly toxic nitro(so) products such as nitro(so)-phenolics and N-nitrosodimethylamine are generated. For a niche application, the UV/chlorine process is recommended to be utilized in water with low levels of DOM and ammonia.Strategies should be developed to make full use of highly reactive species (RCS and HO•) for the abatement of target contaminants and to reduce the formation of toxic byproducts. For example, the UV/chlorine process can be used in tandem with other treatments to create multiple barriers for the production of safe water. In addition, halogen radicals are very important in ecosystems as well as other areas such as medical therapy and organic synthesis. UV/chlorine is th...

show abstract

Comparative removal of two antibiotic resistant bacteria and genes by the simultaneous use of chlorine and UV irradiation (UV/chlorine): Influence of free radicals on gene degradation

Cited by 52 publications

References 43 publications

Assessment of the UV/Chlorine Process in the Disinfection of Pseudomonas aeruginosa: Efficiency and Mechanism

Assessment of the UV/Chlorine Process in the Disinfection of Pseudomonas aeruginosa: Efficiency and Mechanism

Addressing main challenges in the tertiary treatment of urban wastewater: are homogeneous photodriven AOPs the answer?

UV/Chlorine Process: An Efficient Advanced Oxidation Process with Multiple Radicals and Functions in Water Treatment

Contact Info

Product

Resources

About