2002
DOI: 10.1086/342083
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Comparative Rates of Pollination and Fruit Set in Widely Separated Populations of a Rare Orchid (Cypripedium fasciculatum)

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Cited by 26 publications
(15 citation statements)
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“…For each study site, during peak flowering season, we randomly chose ten flowering plants by assigning every plant within a 10 m quadrant a number and choosing plants via a random number generator. The evening prior to the experiment, we chose pairs of mature buds on each plant and bagged them in bridal veil netting following Lipow et al (2002) protocols. Flowers received one of two treatments.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…For each study site, during peak flowering season, we randomly chose ten flowering plants by assigning every plant within a 10 m quadrant a number and choosing plants via a random number generator. The evening prior to the experiment, we chose pairs of mature buds on each plant and bagged them in bridal veil netting following Lipow et al (2002) protocols. Flowers received one of two treatments.…”
Section: Methodsmentioning
confidence: 99%
“…A Zeiss Universal microscope with a 100 W mercury bulb to give fluorescent light was used to view the pollen tubes. The number of pollen grains on the stigma, the number of pollen tubes in the style, and the number of pollen tubes that reached the ovary were all counted to determine successful rates of pollination (see Lipow et al 2002).…”
Section: Methodsmentioning
confidence: 99%
“…These flowers were then collected, fixed, and preserved (sensu Lipow et al 2002). Protocols used to split pistils lengthwise using a razor blade followed Edens-Meier et al (2011) in order to locate pollen tubes on stigmas and/or within pistils.…”
Section: Analyses Of Hand-pollinations (Pollen Tube/pistil Interactiomentioning
confidence: 99%
“…Protocols used to split pistils lengthwise using a razor blade followed Edens-Meier et al (2011) in order to locate pollen tubes on stigmas and/or within pistils. Softening, staining, and observing under epifluorescence followed Lipow et al (2002).…”
Section: Analyses Of Hand-pollinations (Pollen Tube/pistil Interactiomentioning
confidence: 99%
“…The study was conducted within a restricted geographical area of Norfolk County, Ontario,Canada (42º37' to 42º48'N,80º25' to 80º39'W), to reduce the potential problems associated with differences in climate (Lipow et al 2002) and edaphic factors (Mix et al 2006). This region is located in southern Ontario's deciduous forest ecosystem, the Carolinian forest.…”
Section: Study Regionmentioning
confidence: 99%