Comparative Rates of Pollination and Fruit Set in Widely Separated Populations of a Rare Orchid (Cypripedium fasciculatum)

Lipow, Sara R.; Bernhardt, Peter; Vance, Nan C.

doi:10.1086/342083

Cited by 26 publications

(15 citation statements)

References 21 publications

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“…For each study site, during peak flowering season, we randomly chose ten flowering plants by assigning every plant within a 10 m quadrant a number and choosing plants via a random number generator. The evening prior to the experiment, we chose pairs of mature buds on each plant and bagged them in bridal veil netting following Lipow et al (2002) protocols. Flowers received one of two treatments.…”

Section: Methodsmentioning

confidence: 99%

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Molecular Phylogenetics and Reproductive Biology of Oenothera section Kneiffia (Onagraceae)

2014

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Section: Methodsmentioning

confidence: 99%

“…A Zeiss Universal microscope with a 100 W mercury bulb to give fluorescent light was used to view the pollen tubes. The number of pollen grains on the stigma, the number of pollen tubes in the style, and the number of pollen tubes that reached the ovary were all counted to determine successful rates of pollination (see Lipow et al 2002).…”

Section: Methodsmentioning

confidence: 99%

Molecular Phylogenetics and Reproductive Biology of Oenothera section Kneiffia (Onagraceae)

2014

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“…These flowers were then collected, fixed, and preserved (sensu Lipow et al 2002). Protocols used to split pistils lengthwise using a razor blade followed Edens-Meier et al (2011) in order to locate pollen tubes on stigmas and/or within pistils.…”

Section: Analyses Of Hand-pollinations (Pollen Tube/pistil Interactiomentioning

confidence: 99%

“…Protocols used to split pistils lengthwise using a razor blade followed Edens-Meier et al (2011) in order to locate pollen tubes on stigmas and/or within pistils. Softening, staining, and observing under epifluorescence followed Lipow et al (2002).…”

Section: Analyses Of Hand-pollinations (Pollen Tube/pistil Interactiomentioning

confidence: 99%

Floral biology of large-flowered Thelymitra species (Orchidaceae) and their hybrids in Western Australia

Edens-Meier¹,

Westhus²,

Bernhardt³

2013

Telopea

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Historically, only a few large flowered species in the genus Thelymitra were identified as obligate out-breeders. We compared floral presentation, pollen-pistil interactions, pollination ecology, and interspecific hybridization in two populations of T. macrophylla where its flowering periods overlapped with T. antennifera (Tenterden) and T. crinita (Lesmurdie) respectively. Pollen-pistil interactions were studied using glasshouse collections of T. crinita and T. macrophylla at KPBG. The number of flowers per inflorescence in T. macrophylla varied significantly between sites. Climatic conditions influenced flower opening and closing regimes differently in T. crinita vs. T. macrophylla. While all three Thelymitra species opened on warm, sunny mornings and closed by late afternoon, T. crinita at Lesmurdie was significantly more likely to open its perianth segments on cool days compared to the co-blooming, sympatric flowers of T. macrophylla. The floral lifespans of individual flowers of T. macrophylla and T. crinita were reduced significantly following application of Thelymitra pollen onto the stigmatic surface but were not reduced by pollinarium removal. Flowers of both species were self-compatible but neither species self-pollinated mechanically (autogamy). Fluorescence microscopy also showed that both species were inter-compatible. Natural rates of pollinarium removal by insects were low in all three species at both sites. Natural rates of pollen deposition on receptive stigmas were significantly higher in T. crinita vs. T. macrophylla but pollen deposition was less than 12% in both species. Observations and collections of pollinators were infrequent and pollinaria vectors were restricted to a few polylectic, female bees in the families Apidae, Colletidae, and Halictidae. We found a large number of hybrids between T. antennifera and T. macrophylla at Tenterden but few obvious hybrids between T. crinita and T. macrophylla at Lesmurdie. As expected, all hybrids showed characteristics intermediate between their two putative parent species, including pollen configuration in the T. crinita × T. macrophylla specimens. Due to malformations of the column, the majority of T. antennifera × T. macrophylla flowers appeared unable to attach their pollinia to their respective rostella.

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“…The study was conducted within a restricted geographical area of Norfolk County, Ontario,Canada (42º37' to 42º48'N,80º25' to 80º39'W), to reduce the potential problems associated with differences in climate (Lipow et al 2002) and edaphic factors (Mix et al 2006). This region is located in southern Ontario's deciduous forest ecosystem, the Carolinian forest.…”

Section: Study Regionmentioning

confidence: 99%

Effects of Forest Cover on Fruit Set in the Woodland Herb, Maianthemum canadense (Liliaceae)

2008

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. 2008. Effects of forest cover on fruit set in the woodland herb, Maianthemum canadense (Liliaceae). Canadian Field-Naturalist 122(3): 234-238.Animal pollination has been recognized as an essential ecosystem function that is potentially under various environmental stresses. We investigated the landscape effects of forest cover at multiple spatial scales on the sexual reproductive success of a common woodland herb in North America, Maianthemum canadense. This species is a self-incompatible species and pollinated by insects requiring natural landscapes. Nine populations were selected in deciduous forests within agricultural fields of southern Ontario, Canada. We investigated whether fruiting success decreases as forest cover surrounding the plant populations increases at the landscape scale. Forest cover was quantified by the proportion of forest within six different radii from 250 to 1500 m. Analyses showed relationships with the proportion of forest at 750-and 1000-m radii and fruiting success in populations of M. canadense. These findings suggest potential local extirpation of M. canadense and indicate that forest loss can negatively impact on even some common woodland herbs.

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Comparative Rates of Pollination and Fruit Set in Widely Separated Populations of a Rare Orchid (Cypripedium fasciculatum)

Cited by 26 publications

References 21 publications

Molecular Phylogenetics and Reproductive Biology of <I>Oenothera</I> section <I>Kneiffia</I> (Onagraceae)

Molecular Phylogenetics and Reproductive Biology of <I>Oenothera</I> section <I>Kneiffia</I> (Onagraceae)

Floral biology of large-flowered Thelymitra species (Orchidaceae) and their hybrids in Western Australia

Effects of Forest Cover on Fruit Set in the Woodland Herb, <em>Maianthemum canadense</em> (Liliaceae)

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