Comparative proteomics reveals association of early accumulated proteins in conferring butachlor tolerance in three N2-fixing Anabaena spp.

Agrawal, Chitranjan; Sen, Sonia; Singh, Shilpi; Rai, Saurabh; Singh, Prashant Kumar; Singh, Vinay Kumar; Rai, L.C.

doi:10.1016/j.jprot.2013.11.015

Cited by 64 publications

(32 citation statements)

References 82 publications

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“…Proteomic analysis has also been successfully applied to explore the plant phytoremediation mechanism of pyrene by Scirpus triqueter [14] or in the study of algae Anabaena sp. adaptations under butachlor pesticide action [15]. In our earlier work, N-acetyl oxidation was documented as the primary route of alachlor transformation by the microscopic filamentous fungus P. marquandii IM 6003.…”

Section: Introductionmentioning

confidence: 99%

Mechanism study of alachlor biodegradation by Paecilomyces marquandii with proteomic and metabolomic methods

Szewczyk

Soboń

Słaba

et al. 2015

Journal of Hazardous Materials

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Alachlor is a herbicide that is widely used worldwide to protect plant crops against broadleaf weeds and annual grasses. However, due to its endocrine-disrupting activity, its application has been banned in the European Union. As described in our earlier work, Paecilomyces marquandii is a microscopic fungus capable of alachlor removal by N-acetyl oxidation. Our current work employs proteomics and metabolomics to gain a better understanding of alachlor biodegradation by the microscopic fungus P. marquandii. The data revealed that the addition of alachlor reduced culture growth and glucose consumption rate. At the same time, the rates of glycolysis and the tricarboxylic acid (TCA) cycle increased during the initial stage of growth, and there was a shift toward the formation of supplementary materials (UDP-glucose/galactose) and reactive oxygen species (ROS) scavengers (ascorbate). Proteomic analysis revealed that the xenobiotic presence resulted in a strong upregulation of enzymes related to energy, sugar metabolism and ROS production. However, the unique overexpression of cyanide hydratase in alachlor-containing cultures may implicate this enzyme as the key protein involved in the alachlor biodegradation pathway. The characterization of P. marquandii-mediated alachlor removal in terms of cell structure and function resulted in a deeper insight into microorganism strategy toward xenobiotic biodegradation.

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Section: Introductionmentioning

confidence: 99%

Mechanism study of alachlor biodegradation by Paecilomyces marquandii with proteomic and metabolomic methods

Szewczyk

Soboń

Słaba

et al. 2015

Journal of Hazardous Materials

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“…PCC7120 under cadmium stress. Similarly, Agrawal et al (2014) found following trend of tolerant behavior A. L31> Anabaena sp. PCC7120>A. doliolum under butachlor stress among three closely related species of Anabaena.…”

Section: Discussionmentioning

confidence: 69%

Comparative assessment of Ni and As(III) mediated alterations in diazotrophic cyanobacteria, Anabaena doliolum and Anabaena sp. PCC7120

Prajapati¹,

Yadav²,

Atri³

2017

Afr. J. Microbiol. Res.

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The comparative effects of nickel (Ni 2+ ) and arsenite (As(III)) on two diazotrophic cyanobacterial species were investigated in terms of photosynthetic attributes. Both metals demonstrated inhibitory effects on growth, pigments (chl a and phycocyanin) and photosystem II (PS II) photochemistry. However As(III) exerted severe effects as compared to Ni reflected by (1) reduced growth (2) significant inhibition of chl a and phycocyanin, (3) reduction in maximum photochemical efficiency of PSII and (4) depleted plastoquinone pool, thus suggesting it as more toxic. Moreover, comparative analysis of two species also demonstrated interspecies variation in terms of stress adaptive strategies reflected through higher sensitivity of Anabaena doliolum over Anabaena PCC7120. Thus the study recommends application of A. PCC7120 as biofertilizer in Ni and As(III) contaminated paddy fields.

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“…The mode of action of butachlor is by inhibiting the elongase responsible for the elongation of very long-chain fatty acids and the geranylgeranyl pyrophosphate cyclization enzymes (Götz and Böger 2004). It also affects the various other metabolic processes and redox homeostasis adversely, in addition to lipid biosynthesis (Agrawal et al 2014). Butachlor primarily enters the environment through various agricultural, horticultural and forestry practices, where inappropriate water management and rainfall contribute to butachlor runoff from the agricultural fields to the watersheds and aquatic ecosystems (Ok et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Addressing the environmental impacts of butachlor and the available remediation strategies: a systematic review

Abigail

Samuel

Ramalingam

2015

Int. J. Environ. Sci. Technol.

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Butachlor, a chloroacetanilide herbicide, used extensively all over the world as pre-emergence control of unwanted weeds. As a consequence, concerns about its potential adverse effects on the ecosystem and toxicity have risen. Several techniques have been used or are being investigated for effective removal of butachlor from the contaminated sites. This review reports the various toxicological studies conducted so far on the butachlor and the removal technologies available for its decontamination for better understanding. A new insight was also proposed after critical analysis of the merits and demerits of the removal technologies elucidated in the literature. An attempt was made to summarize the currently available analytical techniques reported for determination of butachlor in the environmental samples.

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Comparative proteomics reveals association of early accumulated proteins in conferring butachlor tolerance in three N2-fixing Anabaena spp.

Cited by 64 publications

References 82 publications

Mechanism study of alachlor biodegradation by Paecilomyces marquandii with proteomic and metabolomic methods

Mechanism study of alachlor biodegradation by Paecilomyces marquandii with proteomic and metabolomic methods

Comparative assessment of Ni and As(III) mediated alterations in diazotrophic cyanobacteria, Anabaena doliolum and Anabaena sp. PCC7120

Addressing the environmental impacts of butachlor and the available remediation strategies: a systematic review

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