Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in<i>Salicornia europaea</i>

Nie, Lingling; Feng, Juanjuan; Fan, Pengxiang; Chen, Xianyang; Guo, Jie; Lv, Sulian; Bao, Hexigeduleng; Jia, Wenbao; Tai, Fang; Jiang, Ping; Wang, Jinhui; Li, Yinxin

doi:10.1093/jxb/erv216

Cited by 33 publications

(21 citation statements)

References 63 publications

(78 reference statements)

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“…The proposed nitrate‐dependent pathway for shoot Na + accumulation in Arabidopsis is consistent with previous observations, although in these cases the Na + ‐nitrate interaction is expressed contrariwise: Na + enhancement of nitrate transport. Na + ‐dependent nitrate transport has been described in the halophytes Zostera marina (García‐Sánchez et al ., ), Sueda physophora (Yuan et al ., ), and Salicornia europea (Nie et al ., ), although in the last case the effect was indirect. Also, Na + enhances both nitrate uptake (Kaburagi et al ., ) and root‐to‐leaf nitrate translocation (Kaburagi et al ., ) in Beta vulgaris .…”

Section: Discussionmentioning

confidence: 93%

Nitrate‐dependent shoot sodium accumulation and osmotic functions of sodium in Arabidopsis under saline conditions

Álvarez-Aragón

Rodríguez‐Navarro

2017

The Plant Journal

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Improving crop plants to be productive in saline soils or under irrigation with saline water would be an important technological advance in overcoming the food and freshwater crises that threaten the world population. However, even if the transformation of a glycophyte into a plant that thrives under seawater irrigation was biologically feasible, current knowledge about Na effects would be insufficient to support this technical advance. Intriguingly, crucial details about Na uptake and its function in the plant have not yet been well established. We here propose that under saline conditions two nitrate-dependent transport systems in series that take up and load Na into the xylem constitute the major pathway for the accumulation of Na in Arabidopsis shoots; this pathway can also function with chloride at high concentrations. In nrt1.1 nitrate transport mutants, plant Na accumulation was partially defective, which suggests that NRT1.1 either partially mediates or modulates the nitrate-dependent Na transport. Arabidopsis plants exposed to an osmotic potential of -1.0 MPa (400 mOsm) for 24 h showed high water loss and wilting in sorbitol or Na/MES, where Na could not be accumulated. In contrast, in NaCl the plants that accumulated Na lost a low amount of water, and only suffered transitory wilting. We discuss that in Arabidopsis plants exposed to high NaCl concentrations, root Na uptake and tissue accumulation fulfil the primary function of osmotic adjustment, even if these processes lead to long-term toxicity.

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Section: Discussionmentioning

confidence: 93%

Nitrate‐dependent shoot sodium accumulation and osmotic functions of sodium in Arabidopsis under saline conditions

Álvarez-Aragón

Rodríguez‐Navarro

2017

The Plant Journal

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“…Consequently, the transcriptional repression of these key genes in the sos1 mutant root should have a profound effect on nitrate uptake from the mineral medium and subsequent nitrogen assimilation, as already indicated by the GO term and MapMan analyses. The physiological implications of this response are presently unclear, but might be related to the mutual stimulation of nitrate and Na + uptakes that has been observed in various species including rice (Kaburagi et al, 2015;Nie et al, 2015;Gao et al, 2016). In Arabidopsis, nitratedependent transport systems mediate the uptake and load of Na + into the xylem, and they may constitute a major pathway for the accumulation of Na + in Arabidopsis shoots (Álvarez-Aragón and Rodríguez-Navarro, 2017).…”

Section: Nitrate (Nomentioning

confidence: 99%

A Critical Role of Sodium Flux via the Plasma Membrane Na⁺/H⁺ Exchanger SOS1 in the Salt Tolerance of Rice

et al. 2019

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Rice (Oryza sativa) stands among the world's most important crop species. Rice is salt sensitive, and the undue accumulation of sodium ions (Na +) in shoots has the strongest negative correlation with rice productivity under long-term salinity. The plasma membrane Na + /H + exchanger protein Salt Overly Sensitive 1 (SOS1) is the sole Na + efflux transporter that has been genetically characterized to date. Here, the importance of SOS1-facilitated Na + flux in the salt tolerance of rice was analyzed in a reversegenetics approach. A sos1 loss-of-function mutant displayed exceptional salt sensitivity that was correlated with excessive Na + intake and impaired Na + loading into the xylem, thus indicating that SOS1 controls net root Na + uptake and long-distance Na + transport to shoots. The acute Na + sensitivity of sos1 plants at low NaCl concentrations allowed analysis of the transcriptional response to sodicity stress without effects of the osmotic stress intrinsic to high-salinity treatments. In contrast with that in the wild type, sos1 mutant roots displayed preferential down-regulation of stress-related genes in response to salt treatment, despite the greater intensity of stress experienced by the mutant. These results suggest there is impaired stress detection or an inability to mount a comprehensive response to salinity in sos1. In summary, the plasma membrane Na + /H + exchanger SOS1 plays a major role in the salt tolerance of rice by controlling Na + homeostasis and possibly contributing to the sensing of sodicity stress.

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“…Not surprisingly, there are very few reports about Na +coupled transport systems in terrestrial plants. Na + -dependent nitrate transport has been described in the halophytes Suaeda physophora and Salicornia europea (Junfeng et al, 2010;Nie et al, 2015). In Beta vulgaris, Na + enhances both nitrate uptake and translocation to shoots (Kaburagi et al, 2014(Kaburagi et al, , 2015.…”

Section: Nitrate-sodium Interactionsmentioning

confidence: 99%

Coordinated Transport of Nitrate, Potassium, and Sodium

et al. 2020

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Potassium (K + ) and nitrogen (N) are essential nutrients, and their absorption and distribution within the plant must be coordinated for optimal growth and development. Potassium is involved in charge balance of inorganic and organic anions and macromolecules, control of membrane electrical potential, pH homeostasis and the regulation of cell osmotic pressure, whereas nitrogen is an essential component of amino acids, proteins, and nucleic acids. Nitrate (NO 3 − ) is often the primary nitrogen source, but it also serves as a signaling molecule to the plant. Nitrate regulates root architecture, stimulates shoot growth, delays flowering, regulates abscisic acid-independent stomata opening, and relieves seed dormancy. Plants can sense K + /NO 3 − levels in soils and adjust accordingly the uptake and root-to-shoot transport to balance the distribution of these ions between organs. On the other hand, in small amounts sodium (Na + ) is categorized as a "beneficial element" for plants, mainly as a "cheap" osmolyte. However, at high concentrations in the soil, Na + can inhibit various physiological processes impairing plant growth. Hence, plants have developed specific mechanisms to transport, sense, and respond to a variety of Na + conditions. Sodium is taken up by many K + transporters, and a large proportion of Na + ions accumulated in shoots appear to be loaded into the xylem by systems that show nitrate dependence. Thus, an adequate supply of mineral nutrients is paramount to reduce the noxious effects of salts and to sustain crop productivity under salt stress. In this review, we will focus on recent research unraveling the mechanisms that coordinate the K + -NO 3 − ; Na + -NO 3 − , and K + -Na + transports, and the regulators controlling their uptake and allocation.

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Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake inSalicornia europaea

Abstract: HighlightUnlike in glycophytes, NaCl facilitates the nitrate uptake in the euhalophyte S. europaea, while calcium signalling plays important roles in this process.

Cited by 33 publications

References 63 publications

Nitrate‐dependent shoot sodium accumulation and osmotic functions of sodium in Arabidopsis under saline conditions

Nitrate‐dependent shoot sodium accumulation and osmotic functions of sodium in Arabidopsis under saline conditions

A Critical Role of Sodium Flux via the Plasma Membrane Na⁺/H⁺ Exchanger SOS1 in the Salt Tolerance of Rice

Coordinated Transport of Nitrate, Potassium, and Sodium

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Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake inSalicornia europaea

Abstract: HighlightUnlike in glycophytes, NaCl facilitates the nitrate uptake in the euhalophyte S. europaea, while calcium signalling plays important roles in this process.

Cited by 33 publications

References 63 publications

Nitrate‐dependent shoot sodium accumulation and osmotic functions of sodium in Arabidopsis under saline conditions

Nitrate‐dependent shoot sodium accumulation and osmotic functions of sodium in Arabidopsis under saline conditions

A Critical Role of Sodium Flux via the Plasma Membrane Na+/H+ Exchanger SOS1 in the Salt Tolerance of Rice

Coordinated Transport of Nitrate, Potassium, and Sodium

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A Critical Role of Sodium Flux via the Plasma Membrane Na⁺/H⁺ Exchanger SOS1 in the Salt Tolerance of Rice