Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India

Bhatia, Siddharth; Vasudevan, Karthikeyan

doi:10.1016/j.toxcx.2020.100048

Cited by 24 publications

(25 citation statements)

References 20 publications

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“…The venom’s complexity was expected by referring to the recently analyzed venoms of Pakistani and Indian E. carinatus. − In addition, Coomassie blue staining of the resulting gels revealed a high degree of similarity in the electrophoretic profiles of the three venoms, with all exhibiting polypeptide molecular weight ranges from <10 kDa to around 120 kDa in size. Size-exclusion chromatography (SEC) was applied to decomplex the ECS venom proteome prior to MS measurements, and six fractions were detected and collected from each population of ECS and visualized by SDS-PAGE (Supporting Information Figure S2).…”

Section: Resultssupporting

confidence: 89%

Integrating Top-Down and Bottom-Up Mass Spectrometric Strategies for Proteomic Profiling of Iranian Saw-Scaled Viper, Echis carinatus sochureki, Venom

et al. 2020

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Saw-scaled or carpet vipers (genus Echis) are considered to cause a higher global snakebite mortality than any other snake. Echis carinatus sochureki (ECS) is a widely distributed snake species, also found across the thirteen provinces of Iran, where it is assumed to be responsible for the most snakebite envenomings. Here, we collected the Iranian specimens of ECS from three different geographically distinct populations, investigated food habits, and performed toxicity assessment and venom proteome profiling to better understand saw-scaled viper life. Our results show that the prey items most commonly found in all populations were arthropods, with scorpions from the family Buthidae particularly well represented. LD 50 (median lethal dose) values of the crude venom demonstrate highly comparable venom toxicities in mammals. Consistent with this finding, venom characterization via top-down and bottom-up proteomics, applied to both crude venoms and size-exclusion chromatographic fractions, revealed highly comparable venom compositions among the different populations. By combining all proteomics data, we identified 22 protein families from 102 liquid chromatography and tandem mass spectrometry (LC-MS/MS) raw files, including the most abundant snake venom metalloproteinases (SVMPs, 29−34%); phospholipase A2 (PLA2s, 26−31%); snake venom serine proteinases (SVSPs, 11−12%); L-amino acid oxidases (LAOs, 8−11%), C-type lectins/lectin-like (CTLs, 7−9%) protein families, and many newly detected ones, e.g., renin-like aspartic proteases (RLAPs), fibroblast growth factors (FGFs), peptidyl-prolyl cis-trans isomerases (PPIs), and venom vasodilator peptides (VVPs). Furthermore, we identified and characterized methylated, acetylated, and oxidized proteoforms relating to the PLA2 and disintegrin toxin families and the site of their modifications. It thus seems that post-translational modifications (PTMs) of toxins, particularly target lysine residues, may play an essential role in the structural and functional properties of venom proteins and might be able to influence the therapeutic response of antivenoms, to be investigated in future studies.

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Section: Resultssupporting

confidence: 89%

Integrating Top-Down and Bottom-Up Mass Spectrometric Strategies for Proteomic Profiling of Iranian Saw-Scaled Viper, Echis carinatus sochureki, Venom

et al. 2020

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“…Saw-scaled vipers, Echis genus, are known for their wide distribution from the western African coast, over the Arabian Peninsula to Sri Lanka and Bangladesh [ 5 ]. The intragenus and -species venom variations have been investigated in detail for E. carinatus [ 23 , 76 , 103 ]. Among all other vipers Echis on average displays the highest svMP content (50%), with strong percentual variations between the different species and regions of origin.…”

Section: Venom Variations Of Old World Vipersmentioning

confidence: 99%

Old World Vipers—A Review about Snake Venom Proteomics of Viperinae and Their Variations

Damm

Hempel

Süssmuth

2021

Toxins

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Fine-tuned by millions of years of evolution, snake venoms have frightened but also fascinated humanity and nowadays they constitute potential resources for drug development, therapeutics and antivenoms. The continuous progress of mass spectrometry techniques and latest advances in proteomics workflows enabled toxinologists to decipher venoms by modern omics technologies, so-called ‘venomics’. A tremendous upsurge reporting on snake venom proteomes could be observed. Within this review we focus on the highly venomous and widely distributed subfamily of Viperinae (Serpentes: Viperidae). A detailed public literature database search was performed (2003–2020) and we extensively reviewed all compositional venom studies of the so-called Old-World Vipers. In total, 54 studies resulted in 89 venom proteomes. The Viperinae venoms are dominated by four major, four secondary, six minor and several rare toxin families and peptides, respectively. The multitude of different venomics approaches complicates the comparison of venom composition datasets and therefore we differentiated between non-quantitative and three groups of quantitative workflows. The resulting direct comparisons within these groups show remarkable differences on the intra- and interspecies level across genera with a focus on regional differences. In summary, the present compilation is the first comprehensive up-to-date database on Viperinae venom proteomes and differentiating between analytical methods and workflows.

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“…9 However, the venom profiles differ from other geographically distinct venoms of E. carinatus due to change in the relative composition of the toxin families. 10 Snake venom metalloproteinase, snaclecs and phospholipase A2 are major venom components in all the venoms.…”

Section: Discussionmentioning

confidence: 99%

Saw-scaled viper envenoming complicated with acute myocardial infarction

Pirasath

Gajan

Guruparan

et al. 2021

SAGE Open Medical Case Reports

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The saw-scaled viper ( Echis carinatus) is considered to be a venomous snake which is especially seen in Northern Sri Lanka. Systemic manifestations are rare and reported complications include coagulopathy and renal impairment. The cardiac toxicity following snakebites is rare and cardiac involvement following the saw-scaled viper bites is extremely rare. Here, we describe a patient with acute myocardial infarction following systemic envenoming by saw-scaled viper in Northern Sri Lanka, which was successfully managed per ward protocol following national guidelines.

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Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India

Cited by 24 publications

References 20 publications

Integrating Top-Down and Bottom-Up Mass Spectrometric Strategies for Proteomic Profiling of Iranian Saw-Scaled Viper, Echis carinatus sochureki, Venom

Integrating Top-Down and Bottom-Up Mass Spectrometric Strategies for Proteomic Profiling of Iranian Saw-Scaled Viper, Echis carinatus sochureki, Venom

Old World Vipers—A Review about Snake Venom Proteomics of Viperinae and Their Variations

Saw-scaled viper envenoming complicated with acute myocardial infarction

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