Comparative proteomics of dehydration response in the rice nucleus: New insights into the molecular basis of genotype‐specific adaptation

Abstract

Dehydration is the most crucial environmental factor that considerably reduces the crop harvest index, and thus has become a concern for global agriculture. To better understand the role of nuclear proteins in water-deficit condition, a nuclear proteome was developed from a dehydration-sensitive rice cultivar IR-64 followed by its comparison with that of a dehydration-tolerant c.v. Rasi. The 2DE protein profiling of c.v. IR-64 coupled with MS/MS analysis led to the identification of 93 dehydration-responsive proteins (DRPs). Among those identified proteins, 78 were predicted to be destined to the nucleus, accounting for more than 80% of the dataset. While the detected number of protein spots in c.v. IR-64 was higher when compared with that of Rasi, the number of DRPs was found to be less. Fifty-seven percent of the DRPs were found to be common to both sensitive and tolerant cultivars, indicating significant differences between the two nuclear proteomes. Further, we constructed a functional association network of the DRPs of c.v. IR-64, which suggests that a significant number of the proteins are capable of interacting with each other. The combination of nuclear proteome and interactome analyses would elucidate stress-responsive signaling and the molecular basis of dehydration tolerance in plants.

Comparative analysis of phytochemicals and nutrient availability in two contrasting cultivars of sweet potato (Ipomoea batatas L.)

“…However the outcome is affected by genotypic divergence, organ and tissues, developmental stages besides different environmental cues. The proteomics studies revealed the influence and efficacy of such approach to distinguish the landraces, populations, varieties and even species (Jaiswal et al, 2013;Subba et al, 2013).…”

OsHAD1, a Haloacid Dehalogenase-Like APase, Enhances Phosphate Accumulation

“…Eluted proteins were electrophoresed on 12% SDS-PAGE. Separated protein bands were identified with liquid chromatography-mass spectrometry combined with electrospray ionization as described earlier (Jaiswal et al, 2013).…”

Comparative proteomic analysis of silkworm fat body after knocking out fibroin heavy chain gene: a novel insight into cross-talk between tissues

“…The fat body is the center of nutrients synthesis and metabolism, and can function as a nutrient sensor to coordinate the growth of organism (Colombani et al 2003). Proteomics is an important tool for researchers to extensively investigate proteomic differences by various biologic treatments (Chitteti et al 2008;Coiras et al 2008;de la Tour et al 2013;Jaiswal et al 2013). For example, proteomic analysis of the posterior silk gland with the extremely low fibroin production revealed the molecular mechanism in which the silk production is low ).…”