Comparative Proteomics for Identification of ReproductionRelated Proteins in Testes of the Giant Tiger Shrimp Penaeus monodon

Klinbunga, Sirawut; Petkorn, Sasithorn; Phaonakrop, Narumon; Janpoom, Sirithorn; Prasertlux, Sirikan; Rongmung, Puttawan; Roytrakul, Sittiruk; Menasveta, Piamsak; Khamnamtong, Bavornlak

doi:10.4194/2459-1831-v4_2_02

Cited by 3 publications

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“…The AX-249,988,993 (LG31, pos: 26.457 cM) and AX-249,687,820 (LG31, pos: 28.246 cM) SNPs intercepted the oplophorus-luciferin 2-monooxygenase and serine/arginine repetitive matrix protein genes, respectively. Although these genes are not directly related to sex determination, some studies shown that they are expressed in the gonads of crustaceans, such as Macrobrachium nipponense [ 33 ] and Penaeus monodon [ 34 , 35 ]. Another interesting gene, spermine oxidase , was found close to the AX-249,610,151 SNP (~ 20Kb downstream).…”

Section: Resultsmentioning

confidence: 99%

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A high-density linkage map and sex-determination loci in Pacific white shrimp (Litopenaeus vannamei)

Garcia,

Mastrochirico-Filho,

Gallardo-Hidalgo

et al. 2024

BMC Genomics

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Background Expansion of genomic resources for the Pacific white shrimp (Litopenaeus vannamei), such as the construction of dense genetic linkage maps, is crucial for the application of genomic tools in order to improve economically relevant traits. Sexual dimorphism exists in Pacific white shrimp, and the mapping of the sex-determination region in this species may help in future reproductive applications. We have constructed male, female, and sex-averaged high-density genetic maps using a 50 K single-nucleotide polymorphism (SNP) array, followed by a genome-wide association study (GWAS) to identify genomic regions associated with sex in white shrimp. Results The genetic map yielded 15,256 SNPs assigned to 44 linkage groups (LG). The lengths of the male, female, and sex-averaged maps were 5,741.36, 5,461.20 and 5,525.26 cM, respectively. LG18 was found to be the largest for both sexes, whereas LG44 was the shortest for males and LG31 for females. A sex-determining region was found in LG31 with 21 statistically significant SNPs. The most important SNP was previously identified as a sex-linked marker and was able to identify 99% of the males and 88% of the females. Although other significant markers had a lower ability to determine sex, putative genes were intercepted or close to them. The oplophorus-luciferin 2-monooxygenase, serine/arginine repetitive matrix protein and spermine oxidase genes were identified as candidates with possible participation in important processes of sexual differentiation in shrimp. Conclusions Our results provide novel genomic resources for shrimp, including a high-density linkage map and new insights into the sex-determining region in L. vannamei, which may be usefulfor future genetics and reproduction applications.

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Section: Resultsmentioning

confidence: 99%

“…The second gene was downregulated in the testis and ovary tissues of P. monodon using transcriptomic analysis [ 35 ]. Serine-arginine proteins have been reported to be differentially expressed during the testis maturation process of the same species [ 34 ]. The spermine oxidase gene is closely related to a significant SNP.…”

Section: Discussionmentioning

confidence: 99%

A high-density linkage map and sex-determination loci in Pacific white shrimp (Litopenaeus vannamei)

Garcia,

Mastrochirico-Filho,

Gallardo-Hidalgo

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…The second gene was found downregulated in testis and ovary tissues of P. monodon using transcriptomic analysis [36]. The serine-arginine protein was reported differentially expressed in the testis maturation process of the same species [35]. The spermine oxidase gene was found close to a signi cant SNP.…”

Section: Discussionmentioning

confidence: 99%

“…The AX-249988993 (LG31, pos:26.457 cM) and the AX-249687820 (LG31, pos: 28.246 cM) SNPs intercepted oplophorus-luciferin 2-monooxygenase and serine/arginine repetitive matrix protein genes, respectively. Although these genes are not directly related to sexual determination, some studies found them expressed in the gonads of crustaceans, such as Macrobrachium nipponense [34] and Penaeus monodon [35,36]. Another interesting gene called spermine oxidase was found close to the AX-249610151 SNP (~ 20Kb downstream).…”

Section: Genetic Linkage Mapmentioning

confidence: 99%

A high-density linkage map and sex-determination loci in Pacific white shrimp (Litopenaeus vannamei)

Garcia,

Mastrochirico-Filho,

Gallardo-Hidalgo

et al. 2023

Preprint

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Background Expansion of genomic resources for the Pacific white shrimp (Litopenaeus vannamei) such as the construction of dense genetic linkage maps, is crucial for the application of genomic tools to improve economic relevant traits. Sexual dimorphism exists in Pacific white shrimp, and mapping of the sex determination region in this species may help in the future reproductive applications for this species. We constructed male, female and sex-averaged high-density genetic maps using a 50K single-nucleotide polymorphism (SNP) array followed by a genome-wide association study (GWAS) to identify genomic regions associated to sex in white shrimp.Results The genetic map yielded 15,256 SNPs assigned to 44 linkage groups (LG). The length of the male, female and sex-averaged maps were 5,741.36, 5,461.20 and 5,525.26 cM, respectively. The LG18 was the largest for both sexes while the LG44 was the shortest for males and LG31 for females. A sex determination region was found on LG31 with 21 statistically significant SNPs. The most important SNP was previously identified as a sex-linked marker and it was able to identify 99% of males and 88% of females. Although, the other significant markers had lower ability to determine sex, putative genes were found intercepted or close to them. The oplophorus-luciferin 2-monooxygenase, serine/arginine repetitive matrix protein and spermine oxidase genes were identified as candidate with possible participation in important processes of sexual differentiation in shrimp.Conclusions Our results provided novel genomic resources for shrimp, including a high-density linkage map and new insights regarding the sex determining region in L. vannamei, and may be both useful in future genetics and reproduction applications.

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“…Proteomics is a powerful tool widely used to investigate protein expression and identify potential biomarkers or activated proteins [ 37 ]. This technique can be used to identify proteins regulated by post-translational modification that cannot be detected using genomic or transcriptomic methods [ 32 ]. The aim of the present study was to investigate the effects of cordycepin on protein expression in the brains of streptozotocin (STZ)-induced diabetic mice and clarify the mechanisms underlying its effects on diabetes-associated cognitive impairment.…”

mentioning

confidence: 99%

The effect of cordycepin on brain oxidative stress and protein expression in streptozotocin-induced diabetic mice

Srisuksai

Parunyakul

Phaonakrop

et al. 2021

The Journal of Veterinary Medical Science

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Diabetes mellitus (DM) is characterized by metabolic disorders and psychological deficits, including cognitive decline. Here, we investigated the effect of cordycepin on oxidative stress and protein expression in the brains of diabetic mice. Twenty-four mice were divided into four groups, one comprising untreated healthy mice (N); one comprising healthy mice treated with cordycepin (24 mg/kg body weight) (N+Cor); one comprising untreated DM mice; and one comprising DM mice treated with cordycepin (24 mg/kg body weight) (DM+Cor). After 14 days of treatment, cognitive behavior was assessed using the novel object recognition (NOR) test. The brain levels of oxidative stress markers (glutathione, catalase, and superoxide dismutase) were examined using the respective detection kits.Protein expression in brain tissues was assessed by liquid chromatography with tandem mass spectrometry (LC-MS/MS); the functions of the identified proteins were annotated by PANTHER, while major protein-protein interactions were assessed using STITCH. We found that cordycepin treatment significantly decreased body weight and food and water intake in the DM+Cor group compared with that in the DM group; however, no differences in blood glucose levels were found between the two groups. Cordycepin treatment significantly reversed cognitive decline in diabetic mice in the NOR test and ameliorated antioxidant defenses. Additionally, we identified ULK1 isoform 2, a protein associated with cognitive function via the activated AMPK and autophagic pathways, as being uniquely expressed in the DM+Cor group. Our findings provide novel insights into the cellular mechanisms underlying how cordycepin improves cognitive decline in diabetic mice.

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Comparative Proteomics for Identification of ReproductionRelated Proteins in Testes of the Giant Tiger Shrimp Penaeus monodon

Cited by 3 publications

References 0 publications

A high-density linkage map and sex-determination loci in Pacific white shrimp (Litopenaeus vannamei)

A high-density linkage map and sex-determination loci in Pacific white shrimp (Litopenaeus vannamei)

A high-density linkage map and sex-determination loci in Pacific white shrimp (Litopenaeus vannamei)

The effect of cordycepin on brain oxidative stress and protein expression in streptozotocin-induced diabetic mice

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