Comparative proteomics and metabolomics of JAZ7-mediated drought tolerance in Arabidopsis

Li, Meng; Zhang, Tong; Geng, Sisi; Scott, Peter; Li, Haiying; Chen, Sixue

doi:10.1016/j.jprot.2019.02.001

Cited by 44 publications

(27 citation statements)

References 59 publications

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“…It is worth noting that ClJAZ1 and ClJAZ7 showed the most remarkable increases in expression under JA and drought treatments (Figures 7 and 9), indicating that they may play essential roles in regulating the response to drought stress by the JA-mediated signaling pathway. ClJAZ1 was clustered together with AtJAZ7 in the JAZ V group (Figure 1), and overexpression of AtJAZ7 was found to confer drought tolerance in Arabidopsis [50]. Under salt treatment, ClJAZ7 exhibited the highest expression among the detected ClTIFYs (Figure 8), demonstrating that it may play a major role in salt stress response.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of TIFY Transcription Factors and Their Expression Profiles under Jasmonic Acid and Abiotic Stresses in Watermelon

Yang

Ahammed

Wan

et al. 2019

International Journal of Genomics

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The TIFY gene family is plant-specific and encodes proteins involved in the regulation of multiple biological processes. Here, we identified 15 TIFY genes in the watermelon genome, which were divided into four subfamilies (eight JAZs, four ZMLs, two TIFYs, and one PPD) in the phylogenetic tree. The ClTIFY genes were unevenly located on eight chromosomes, and three segmental duplication events and one tandem duplication event were identified, suggesting that gene duplication plays a vital role in the expansion of the TIFY gene family in watermelon. Further analysis of the protein architectures, conserved domains, and gene structures provided additional clues for understanding the putative functions of the TIFY family members. Analysis of qRT-PCR and RNA-seq data revealed that the detected ClTIFY genes had preferential expression in specific tissues. qRT-PCR analysis revealed that nine selected TIFY genes were responsive to jasmonic acid (JA) and abiotic stresses including salt and drought. JA activated eight genes and suppressed one gene, among which ClJAZ1 and ClJAZ7 were the most significantly induced. Salt and drought stress activated nearly all the detected genes to different degrees. These results lay a foundation for further functional characterization of TIFY family genes in Citrullus lanatus.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of TIFY Transcription Factors and Their Expression Profiles under Jasmonic Acid and Abiotic Stresses in Watermelon

Yang

Ahammed

Wan

et al. 2019

International Journal of Genomics

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“…The overexpression of different JAZ genes has been also utilized for manipulating drought tolerance in other plants. For instance, overexpression of JAZ7 in Arabidopsis (Meng et al, 2019) and OsJAZ1 in rice result in increased and decreased drought tolerance, respectively (Fu et al, 2017). The candidates that affect the level of active JA are also targeted for drought tolerance improvement.…”

Section: Jasmonates Pathway Engineeringmentioning

confidence: 99%

Crosstalk between phytohormones and secondary metabolites in the drought stress tolerance of crop plants: A review

Jogawat

Yadav

Chhaya

et al. 2021

Physiologia Plantarum

233

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Drought stress negatively affects crop performance and weakens global food security. It triggers the activation of downstream pathways, mainly through phytohormones homeostasis and their signaling networks, which further initiate the biosynthesis of secondary metabolites (SMs). Roots sense drought stress, the signal travels to the above-ground tissues to induce systemic phytohormones signaling. The systemic signals further trigger the biosynthesis of SMs and stomatal closure to prevent water loss. SMs primarily scavenge reactive oxygen species (ROS) to protect plants from lipid peroxidation and also perform additional defense-related functions.Moreover, drought-induced volatile SMs can alert the plant tissues to perform drought stress mitigating functions in plants. Other phytohormone-induced stress responses include cell wall and cuticle thickening, root and leaf morphology alteration, and anatomical changes of roots, stems, and leaves, which in turn minimize the oxidative stress, water loss, and other adverse effects of drought. Exogenous applications of phytohormones and genetic engineering of phytohormones signaling and biosynthesis pathways mitigate the drought stress effects. Direct modulation of the SMs biosynthetic pathway genes or indirect via phytohormones' regulation provides drought tolerance. Thus, phytohormones and SMs play key roles in plant development under the drought stress environment in crop plants. | INTRODUCTIONA large segment of the population is going to face food scarcity due to climate change and the gradually decreasing arable land area. Plants are confronted to a variable environment while growing from seedling to mature plant. Different abiotic and biotic stresses affect plant growth and development (Cramer et al., 2011;Fujita et al., 2006;Tuteja & Sopory, 2008). Abiotic stress includes drought, submergence, osmotic stress, salinity stress, oxidative stress, ultra-violet irradiation, wounding, and nutrient depletion. The extremity of optimum factors such as high temperature or low temperature and freezing-thawing is also included in abiotic stresses. Biotic stresses include viruses, bacteria, fungi, algae, worms, nematodes, insects, herbivores, and parasitic plants. Plants have to combat these stressors due to their sessile lifestyle (Cramer et al., 2011;Fujita et al., 2006). More than 50% reduction in average yields of major cereal crops has been reported because of various abiotic stresses. Drought is one of the most important abiotic stresses that negatively influence plant performance and causes harsh effects on biomass and yield (Fahad et al., 2017). A

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“…Proteomics, which is the study of the structural and functional features of all the proteins in an organism, is an important means of understanding complex biological mechanisms, including plant responses to abiotic stress tolerance (Deshmukh et al, 2014). Using transcriptomic or proteomics sequencing, many genes associated with drought response were identified in a number of plants, such as Arabidopsis (Cho et al, 2013; Matsui et al, 2008; Meng et al, 2019), rice (Hamzelou et al, 2020; Li et al, 2019), wheat (Fotovat et al, 2017; Kang et al, 2012; Ma et al, 2017; Michaletti et al, 2018), maize (Zeng et al, 2019; Zheng et al, 2020), soybean (Song, Prince, et al, 2016; Wang & Komatsu, 2018), tobacco (Chen et al, 2019; Khan et al, 2019), and others (An et al, 2020; Ghatak et al, 2017; Jangpromma et al, 2010; Ngara & Ndimba, 2013; Raney et al, 2014; Rodziewicz et al, 2019; Singh et al, 2017; Utsumi et al, 2012; Wisniewski et al, 2009; Yang et al, 2017). In rapeseed, numerous candidate genes associated with drought response have also been identified by transcriptomic and proteomics analyses.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and proteome analyses of the molecular mechanisms underlying changes in oil storage under drought stress in Brassica napus L.

Zhang

et al. 2021

GCB Bioenergy

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Rapeseed (Brassica napus L.) is the second most important oilseed crop in edible vegetable oil and bioenergy; however, drought stress generally causes a decrease in rapeseed yield and oil content, especially during the reproductive stage. In our study, we measured the oil and protein contents and gibberellic acid (GA) and abscisic acid (ABA) levels in seeds that were acquired on the 30th, 40th, and 50th days after flowering under control and drought treatments. RNA and protein libraries were constructed from the stressed seeds to perform transcriptome and proteome analyses, respectively. Our results demonstrated that the oil content decreased due to four primary mechanisms: downregulation of fatty acid biosynthesis‐associated genes and proteins; upregulation of fatty acid degradation‐associated genes and proteins; enhancement of protein storage due to changes in the abundances of relevant genes and proteins; and upregulation of Gly‐Asp‐Ser‐Leu (GDSL) gene expression, potentially as the result of upregulating the GA biosynthesis gene GA20ox3 and downregulating the GA inactivating gene GA2ox3 and thus an increase in GA content. During seed maturation, oil storage change may also relate to increasing ABA content as the upregulation of two members of NCED6 (9‐cis‐epoxycarotenoid dioxygenase) gene family involved in ABA biosynthesis, and the upregulation of genes involved in ABA signal transduction. These results will help to establish a foundation for breeding excellent varieties of rapeseed with high oil content for areas with frequent droughts to promote the supply of edible vegetable oil and biofuel.

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Comparative proteomics and metabolomics of JAZ7-mediated drought tolerance in Arabidopsis

Cited by 44 publications

References 59 publications

Comprehensive Analysis of TIFY Transcription Factors and Their Expression Profiles under Jasmonic Acid and Abiotic Stresses in Watermelon

Comprehensive Analysis of TIFY Transcription Factors and Their Expression Profiles under Jasmonic Acid and Abiotic Stresses in Watermelon

Crosstalk between phytohormones and secondary metabolites in the drought stress tolerance of crop plants: A review

Transcriptome and proteome analyses of the molecular mechanisms underlying changes in oil storage under drought stress in Brassica napus L.

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