Comparative proteomics analysis of microvesicles in human serum for the evaluation of osteoporosis

Abstract

Osteoporosis is an emerging health issue worldwide. Due to the decrease of bone mineral density and the deterioration of skeletal microarchitecture, osteoporosis could lead to increased bone fragility and higher fracture risk. Since lack of specific symptoms, novel serum proteomic indicators are urgently needed for the evaluation of osteoporosis. Microvesicles (MVs) are important messengers widely present in body fluids and have emerged as novel targets for the diagnosis of multiple diseases. In this study, MVs were successfully isolated from human serum and comprehensively characterized. Comparative proteomics analysis revealed differential MVs protein profiling in normal subjects, osteopenia patients, and osteoporosis patients. In total, about 200 proteins were identified and quantified from serum MVs, among which 19 proteins were upregulated (fold change >2) and five proteins were downregulated (fold change <0.5) in osteopenia group and osteoporosis group when compared with the normal group. Three protein candidates were selected for initial verification, including Vinculin, Filamin A, and Profilin 1. Profilin 1 was further pre‐validated in an independent sample set, which could differentiate osteoporosis group from osteopenia group and normal group (p < 0.05). Our data collectively demonstrate that serum MVs proteome can be valuable indicators for the evaluation and diagnostics of bone loss disease.

Proteomic profiling analysis of postmenopausal osteoporosis and osteopenia identifies potential proteins associated with low bone mineral density

“…There are several studies that have analyzed the quantitative proteome profiles and explored particular BMD-related proteins or genes based on peripheral blood monocytes (Shao et al, 2019; Zhang et al, 2016). Recently, a comparative proteomics analysis of serum microvesicles for the evaluation of OP was reported, and the authors found that Profilin 1 could differentiate those in the OP group from those in the ON and normal groups (Huo, Li & Qiao, 2019). Our study is the first to investigate plasma proteomic profiling in Chinese postmenopausal women with OP, ON, and normal bone mass using TMT and PRM approaches.…”

Peer Review #3 of "Proteomic profiling analysis of postmenopausal osteoporosis and osteopenia identifies potential proteins associated with low bone mineral density (v0.1)"

“…There are several studies that have analyzed the quantitative proteome profiles and explored particular BMD-related proteins or genes based on peripheral blood monocytes (Shao et al, 2019;Zhang et al, 2016). Recently, a comparative proteomics analysis of serum microvesicles for the evaluation of OP was reported, and the authors found that Profilin 1 could differentiate those in the OP group from those in the ON and normal groups (Huo et al, 2019).…”

The Role of Extracellular Vesicles in Osteoporosis: A Scoping Review

“…Profilin 1 was further prevalidated in independent sample sets, which could be distinguished between the osteoporosis group, osteopenia group, and normal group ( p < 0.05). Their data indicated that the serum microvesicles proteome can be used for evaluation and diagnosis as important indicators of bone-loss disease [ 32 ]. In addition, Ming Chen and others from China 301 Hospital compared the protein profiles of plasma exosomes from 60 patients with osteoporosis, osteopenia, and normal bone mass, aiming to find potential new diagnoses.…”