Comparative proteomics analysis of differentially expressed proteins in soybean cell wall during flooding stress

Abstract

Flooding is a major problem for soybean crop as it reduces the growth and grain yield. To investigate the function of the soybean cell wall in the response to flooding stress, cell wall proteins were analyzed. Cell wall proteins from roots and hypocotyls of soybeans, which were germinated for 2 days and subjected to 2 days of flooding, were purified, separated by two-dimensional polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. Sixteen out of 204 cell wall proteins showed responses to flooding stress. Of these, two lipoxygenases, four germin-like protein precursors, three stem 28/31 kDa glycoprotein precursors, and one superoxide dismutase [Cu-Zn] were downregulated. A copper amine oxidase was found to have shifted from the basic to acidic zone following flooding stress. Based on these results, it was confirmed by the lignin staining that the lignification was suppressed in the root of soybean under the flooding stress. These results suggest that the roots and hypocotyls of soybean caused the suppression of lignification through decrease of these proteins by downregulation of reactive oxygen species and jasmonate biosynthesis under flooding stress.

Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions

“…The CRGs were chosen based on studies of gene expression under hypoxic conditions, such as the 18S gene analyzed in Arabidopsis (Liu et al, 2005) and soybeans (Komatsu et al, 2010); a number of genes validated as soybean reference genes, including ELF1B, TUA, and TUA2 in different tissues at different developmental stages (Jian et al, 2008); and ACTB in drought-stressed soybean roots and leaves (Stolf-Moreira et al, 2011).…”

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

“…A comparison of the 2-DE gel patterns showed that, under stress, a copper amine oxidase protein shifted from the basic zone to the acidic zone. Komatsu et al (2010a) also reported that lignification is suppressed in the roots of soybean following flooding stress. Several studies have identified ROS scavengers as common flooding-responsive proteins in total protein and cell wall fractions of soybean, and various ROS scavengers have been reported to be down-regulated under flooding stress in soybean seedlings (Alam et al, 2010a;Hashiguchi et al, 2009;Komatsu et al, 2009b;Komatsu et al, 2010aShi et al, 2008).…”

An anatomical study of developmental changes in maturing root tissues of pomegranate (Punica granatum L.) and formation of a unique type of periderm

“…Root tissue density or tissue volume is a matter of prime interest anatomically and it is important when studying adaptive responses of plants to their environmental conditions. Since root cell wall is the first to be affected by any stress signal (Komatsu et al 2010, Salvador et al 2013), studies on cell wall modification and their function deserve more attention. In this study we uncovered a unique layout of lingo-suberic biopolymers in pomegranate roots from growth to maturity which may be a highly species specific matter.…”