Comparative proteomics analysis of differential proteins in respond to doxorubicin resistance in myelogenous leukemia cell lines

Shi, Qinghong; Gao, Shen; Song, Lina; Zhao, Yueming; Li, Xiaoou; Wu, Jian‐Lin; He, Chengyan; Zhang, Dongliang; Zhao, Haifeng

doi:10.1186/s12953-014-0057-y

Cited by 49 publications

(44 citation statements)

References 48 publications

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“…Indeed, several studies in different cancer models have demonstrated an efficient suppression of MDR by glycolytic inhibitors823244445. Moreover, during the course of this work another study has suggested the possibility of an accelerated process of glycolysis in MDR cells to increase their energy supply46. To our knowledge, a comparison between MDR and drug-sensitive cells in terms of metabolic energy supply, using different modulators of glycolysis and OXPHOS, has never been performed.…”

Section: Discussionmentioning

confidence: 84%

Identification of the metabolic alterations associated with the multidrug resistant phenotype in cancer and their intercellular transfer mediated by extracellular vesicles

Lopes-Rodrigues

Luca

Mleczko

et al. 2017

Sci Rep

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Multidrug resistance (MDR) is a serious obstacle to efficient cancer treatment. Overexpression of P-glycoprotein (P-gp) plays a significant role in MDR. Recent studies proved that targeting cellular metabolism could sensitize MDR cells. In addition, metabolic alterations could affect the extracellular vesicles (EVs) cargo and release. This study aimed to: i) identify metabolic alterations in P-gp overexpressing cells that could be involved in the development of MDR and, ii) identify a potential role for the EVs in the acquisition of the MDR. Two different pairs of MDR and their drug-sensitive counterpart cancer cell lines were used. Our results showed that MDR (P-gp overexpressing) cells have a different metabolic profile from their drug-sensitive counterparts, demonstrating decreases in the pentose phosphate pathway and oxidative phosphorylation rate; increases in glutathione metabolism and glycolysis; and alterations in the methionine/S-adenosylmethionine pathway. Remarkably, EVs from MDR cells were capable of stimulating a metabolic switch in the drug-sensitive cancer cells, towards a MDR phenotype. In conclusion, obtained results contribute to the growing knowledge about metabolic alterations in MDR cells and the role of EVs in the intercellular transfer of MDR. The specific metabolic alterations identified in this study may be further developed as targets for overcoming MDR.

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Section: Discussionmentioning

confidence: 84%

Identification of the metabolic alterations associated with the multidrug resistant phenotype in cancer and their intercellular transfer mediated by extracellular vesicles

Lopes-Rodrigues

Luca

Mleczko

et al. 2017

Sci Rep

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“…Salivation plays an important role in food consumption and digestion in rodents [37], and have roles in host defense [38]. Thus, salivary gland impairment may potentially impact food intake, body weight and some innate defense mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Carotid Catheterization and Automated Blood Sampling Induce Systemic IL-6 Secretion and Local Tissue Damage and Inflammation in the Heart, Kidneys, Liver and Salivary Glands in NMRI Mice

et al. 2016

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Automated blood sampling through a vascular catheter is a frequently utilized technique in laboratory mice. The potential immunological and physiological implications associated with this technique have, however, not been investigated in detail. The present study compared plasma levels of the cytokines IL-1β, IL-2, IL-6, IL-10, IL-17A, GM-CSF, IFN-γ and TNF-α in male NMRI mice that had been subjected to carotid artery catheterization and subsequent automated blood sampling with age-matched control mice. Body weight and histopathological changes in the surgical area, including the salivary glands, the heart, brain, spleen, liver, kidneys and lungs were compared. Catheterized mice had higher levels of IL-6 than did control mice, but other cytokine levels did not differ between the groups. No significant difference in body weight was found. The histology revealed inflammatory and regenerative (healing) changes at surgical sites of all catheterized mice, with mild inflammatory changes extending into the salivary glands. Several catheterized mice had multifocal degenerative to necrotic changes with inflammation in the heart, kidneys and livers, suggesting that thrombi had detached from the catheter tip and embolized to distant sites. Thus, catheterization and subsequent automated blood sampling may have physiological impact. Possible confounding effects of visceral damage should be assessed and considered, when using catheterized mouse models.

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“…Samples were loaded onto an IPG strips with linear PH gradient 4–7 and 24 cm length (GE Healthcare, Uppsala, Sweden). After hydrated, the strips were performed isoelectric focusing (IEF) and SDS-PAGE gels electrophoresis to separate proteins as described48.…”

Section: Methodsmentioning

confidence: 99%

“…Subcellular localization was predicted using CELLO V.2.5 (http://cello.life.nctu.edu.tw). Identified proteins were further analyzed using the STRING V10.0 database (http://string-db.org/) for PPI network analysis, to statistically determine the functions and pathways most strongly associated with the protein list48. KEGG (http://www.genome.jp/kegg/pathway) pathway analysis was performed to determine their molecular interaction and reaction networks52.…”

Section: Methodsmentioning

confidence: 99%

Comparative Proteomics Reveals that Phosphorylation of β Carbonic Anhydrase 1 Might be Important for Adaptation to Drought Stress in Brassica napus

Wang

Jin

et al. 2016

Sci Rep

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Little is known about the mechanism of drought tolerance in rapeseed (Brassica napus L.). In this study, different morphological and physiological responses to drought stress were studied in three rapeseed cultivars. For the cultivar 2AF009 with high drought tolerance, comparative proteomic analyses were conducted to determine the molecular mechanism behind. Approximately 138 differentially abundant proteins (DAPs) and 1232 phosphoproteins containing 4469 phosphopeptides were identified. Furthermore, 337 phosphoproteins containing 547 phosphorylation sites demonstrated significant changes. These drought-responsive DAPs and phosphoproteins were mainly involved in signal transduction, photosynthesis, and glutathione-ascorbate metabolism. Notably, 9 DAPs were also identified as drought-responsive phosphoproteins, especially beta carbonic anhydrase 1 (βCA1), which was represented by eight distinct protein spots with different abundant levels during drought stress. Tyr207 phosphorylated site of βCA1 was down-regulated at the phosphorylation level during drought stress, which was also located in the substrate-binding active region of three-dimensional (3D) structure. Moreover, drought stress inhibited CA activity. We concluded that Tyr207 was the most likely phosphorylation target affecting the enzyme activity, and phosphorylation of βCA1 might be important for the response to drought stress in rapeseed. The study provided a new clue for the drought tolerance mechanism in B.napus.

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Comparative proteomics analysis of differential proteins in respond to doxorubicin resistance in myelogenous leukemia cell lines

Cited by 49 publications

References 48 publications

Identification of the metabolic alterations associated with the multidrug resistant phenotype in cancer and their intercellular transfer mediated by extracellular vesicles

Identification of the metabolic alterations associated with the multidrug resistant phenotype in cancer and their intercellular transfer mediated by extracellular vesicles

Carotid Catheterization and Automated Blood Sampling Induce Systemic IL-6 Secretion and Local Tissue Damage and Inflammation in the Heart, Kidneys, Liver and Salivary Glands in NMRI Mice

Comparative Proteomics Reveals that Phosphorylation of β Carbonic Anhydrase 1 Might be Important for Adaptation to Drought Stress in Brassica napus

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