Comparative proteomic study of phytotoxic effects of silver nanoparticles and silver ions on tobacco plants

Štefanić, Petra Peharec; Jarnević, Martina; Cvjetko, Petra; Biba, Renata; Šikić, Sandra; Tkalec, Mirta; Cindrić, Mario; Letofsky-Papst, Ilse; Balen, Biljana

doi:10.1007/s11356-019-05552-w

Cited by 24 publications

(12 citation statements)

References 52 publications

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“…The mechanisms explaining the impact of AgNPs on germination and plant biomass are still not completely elucidated. Positive effects can be ascribed to AgNPs ability to generate new pores on the seed coat during penetration, which may help to influx the nutrients inside the seed or AgNPs may carry the nutrients along with them, leading to accelerated germination and increased growth rate (Tkalec et al 2019). On the other hand, Zuverza-Mena et al (2016) demonstrated that AgNP-treatment of radish sprouts induced a decrease in water content in a dose-dependent manner, which is in accordance with increased dry matter content after AgNPexposure of tobacco seedlings recorded in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown both positive and negative impacts of AgNPs on plant metabolism, mainly depending on their concentration, size, shape and coating (Pallavi et al 2016, Jasim et al 2017, Cvjetko et al 2017, Peharec Štefanić et al 2019. Experimental methodology (growth medium, exposure method, exposure time) as well as plant system used (species and developmental stage) also significantly affect AgNP phytotoxicity (Yan and Chen 2019).…”

Section: Introductionmentioning

confidence: 99%

“…AgNP suspensions are prone to chemical transformations (oxidation, dissolution, aggregation and agglomeration) (Levard et al 2012, Gorham et al 2014 making it harder to determine whether the effects of AgNPs are purely nanoparticle-related or a consequence of silver ion release from the nanoparticles. So far, research has provided evidence for both Ag + ion-and AgNP-specific toxicity (Tkalec et al 2019). Ag + ions released from AgNPs can induce oxidative stress through excessive reactive oxygen species (ROS) production (Park et al 2009), disturb cell function by binding to cell components and modifying their activities (Montes et al 2017) and affect photosynthesis through competitive substitution of Cu + ions in plastocyanin (Sujak 2005, Jansson andHansson 2008).…”

Section: Introductionmentioning

confidence: 99%

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Silver nanoparticles affect germination and photosynthesis in tobacco seedlings

Biba

Tkalec

Cvjetko

et al. 2020

Acta bot. Croat. (Online)

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Extensive commercialization of silver nanoparticles (AgNPs) raises the risk of their accumulation in the soil-plant system. Once released into the environment, AgNPs are prone to chemical transformations, which makes it hard to determine whether their phytotoxic effects are purely NP-related or a consequence of released Ag+ ions. In this study the effects of 25, 50, 75, 100 and 150 μM AgNPs and AgNO3 on seed germination and early growth of tobacco (Nicotiana tabacum L.) seedlings were compared. Additionally, the effect on photosynthetic performance and pigment content were investigated. Germination rate and index values indicated delayed and slower germination in some AgNP treatments. Lower AgNP concentrations stimulated root growth, but induced prominent reduction in fresh weight. Contrary, all AgNO3 concentrations inhibited root growth but only the higher ones decreased fresh weight. Obtained results imply that the observed AgNP toxicity could be ascribed to NP form and can be correlated with high AgNP stability in the solid medium. On the other hand, majority of AgNP and AgNO3 treatments induced an increase in chlorophyll content which was accompanied with significantly lower values of relative electron transport rate and coefficient of photochemical quenching, implying an inhibition of the electron transport chain. Similar impact of AgNPs and AgNO3 on photosynthesis can be correlated with lower stability of AgNPs in the liquid medium, resulting in AgNP aggregation and dissolution of Ag+ ions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Silver nanoparticles affect germination and photosynthesis in tobacco seedlings

Biba

Tkalec

Cvjetko

et al. 2020

Acta bot. Croat. (Online)

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“…Many studies have already shown AgNP phytotoxic effects that have been mainly attributed to the excess production of reactive oxygen species (ROS), leading to increased oxidative damage to lipids, proteins, and DNA [1,[17][18][19]. However, studies that investigated the impact of AgNPs on plant germination, growth, and morphology showed both stimulatory [20,21] and detrimental [22,23] effects.…”

Section: Introductionmentioning

confidence: 99%

“…In the present study we have investigated the stability of differently coated AgNPs in a nutrient medium, as well as their effect on germination and early growth of tobacco (Nicotiana tabacum L.), an important crop plant that is commonly used as a model organism in abiotic stress research [18,19,25,26]. Tobacco seeds were sown on a nutrient media supplemented with the same concentrations of laboratory-synthesised AgNPs stabilised with two types of surface coatings, polyvinylpyrrolidone (PVP; AgNP-PVP) and cetyltrimethylammonium bromide (CTAB; AgNP-CTAB).…”

Section: Introductionmentioning

confidence: 99%

Coating-Dependent Effects of Silver Nanoparticles on Tobacco Seed Germination and Early Growth

Biba

Matić

Lyons

et al. 2020

IJMS

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Silver nanoparticles (AgNPs) are used in a wide range of consumer products because of their excellent antimicrobial properties. AgNPs released into the environment are prone to transformations such as aggregation, oxidation, or dissolution so they are often stabilised by coatings that affect their physico-chemical properties and change their effect on living organisms. In this study we investigated the stability of polyvinylpyrrolidone (PVP) and cetyltrimethylammonium bromide (CTAB) coated AgNPs in an exposure medium, as well as their effect on tobacco germination and early growth. AgNP-CTAB was found to be more stable in the solid Murashige and Skoog (MS) medium compared to AgNP-PVP. The uptake and accumulation of silver in seedlings was equally efficient after exposure to both types of AgNPs. However, AgNP-PVP induced only mild toxicity on seedlings growth, while AgNP-CTAB caused severe negative effects on all parameters, even compared to AgNO3. Moreover, CTAB coating itself exerted negative effects on growth. Cysteine addition generally alleviated AgNP-PVP-induced negative effects, while it failed to improve germination and growth parameters after exposure to AgNP-CTAB. These results suggest that the toxic effects of AgNP-PVP are mainly a consequence of release of Ag+ ions, while phytotoxicity of AgNP-CTAB can rather be ascribed to surface coating itself.

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