Comparative Proteomic Profiles of Typical Strain and Genetically Altered Variant of Vibrio cholerae O1, Biovar El Tor

Abstract

Objective of the study was to identify the differences in the production of proteins in typical strain and genetically altered variant of V. cholerae O1, biovar El Tor.Materials and methods. Natural strains M1062 (Astrakhan, 1970) and M1509 (Moscow, 2010) were used as model strains in this work. Strains were cultivated on Luria Bertani agar at 37 °C. Electrophoresis was performed in accordance with W.K. Laemmli technique (1970), mass-spectrometric profiling – the method described by A. Shevchenko et al.Results and discussion. Mass-spectrometric scanning of cell lysates of the examined strains showed significant similarity of their proteomes (615 common proteins). The identified differences pertained to high expression of proteins in the strain M1062, participating in biosynthesis of DNA/RNA, included into “purines, pyrimidines, nucleosides” group, as well as regulatory proteins. In M1509 strain, biosynthesis of the proteins responsible for pathogenesis, adaptation under the influence of unfavorable environmental factors, included into “co-factors, vitamins, pigments” group, involved in lipid, carbohydrate, and protein metabolism, cellular processes, as well as proteins-transporters was increased. It has been suggested that the wide dissemination of El Tor genovariants is probably due to enhanced pathogenic and adaptive properties and also to the considerable transformation of cell metabolism.

Vibrio cholerae secretion system of the type VI

“…ранее нами было проведено сравнительное масс-спектрометрическое сканирование белков из лизатов клеток двух токсигенных клинических штаммов V. cholerae о1-серогруппы биовара эль тор: типичного м1062 (астрахань, 1970) и генетически измененного м1509 (москва, 2012), выращенных на LB-агаре при температуре 37 °с. среди 787 белков типичного штамма м1062 действительно не выявлено ни одного белка сс6т, в то же время у генетически измененного штамма м1509 обнаружено два белка: VipA и VipB, -участвующих в формировании внешней трубки [35]. возможно, в циркулирующих в настоящее время генетически измененных штаммах V. cholerae о1 биовара эль тор произошло изменение регуляторных механизмов, что способствовало активации сс6т, и продукция белков-эффекторов происходит при культивировании данных штаммов в лабораторных условиях, но для проверки данного предположения необходимы дополнительные исследования.…”