Comparative proteomic and transcriptomic analysis reveals high pH–induced expression signatures of Chinese shrimp Fenneropenaeus chinensis

Li, Zhaoxia; Tang, Xiaoqi; Li, Jian; Ya, HE

doi:10.1007/s10142-021-00779-8

Cited by 16 publications

(2 citation statements)

References 68 publications

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“…cells. As a component of innate immunity, the chemokine signaling pathway plays multiple roles in immune responses, which could help shrimps to survive under various stresses (Yu et al, 2019;Li et al, 2021). Rap1 and hippo signaling pathways are involved in the regulation of a range of immune functions.…”

Section: Discussionmentioning

confidence: 99%

Identification of microRNAs in black tiger shrimp (Penaeus monodon) under acute low-salinity stress

Shi,

Jiang,

Yang

et al. 2024

Front. Mar. Sci.

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Salinity is a common abiotic stress in the culture of penaeid shrimp. Through post-transcriptional regulation of gene transcripts, microRNAs (miRNAs) play an important role in the adaptation to a stressful environment. However, the involvement of miRNAs in the salinity stress response of shrimp remains unclear. In the present study, the sequence and expression profile of miRNAs in the hepatopancreas of low-salinity-treated Penaeus monodon were obtained by the high-throughput sequencing technique. A total of 679 miRNAs were identified, including 167 miRNAs that were significantly differentially expressed after low-salinity exposure (p < 0.05). Remarkably, most of these miRNAs were downregulated, suggesting that a series of genes were activated to participate in stress response. In addition, 43 miRNAs differentially expressed at all treatment were selected as putative key modulators. Enrichment analysis of genes targeted by these miRNAs indicated that a network that consists of the nervous system, the immune system, and the endocrine system played a crucial role in maintaining the homeostasis of P. monodon under low-salinity stress. These findings may help contribute to a better understanding of the mechanism that regulates salinity tolerance in shrimp and provide valuable genetic information for subsequent studies.

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Section: Discussionmentioning

confidence: 99%

Identification of microRNAs in black tiger shrimp (Penaeus monodon) under acute low-salinity stress

Shi,

Jiang,

Yang

et al. 2024

Front. Mar. Sci.

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“…The pH is an important environmental factor affecting the life of aquatic crustaceans ( Han et al, 2018a ). In general, the effects of low pH on shrimps have received considerable attentions, however, the high pH caused by contamination of water, red tide, elevated photosynthesis rates that occur during the afternoon or saline-alkaline water is also very common in the shrimp farming pond and aquaculture systems ( Li et al, 2021 ). Especially, the pH of water in aquaculture farms can increase dramatically (potentially reaching values greater than pH 9.5) due to microalgal over-multiplication during the middle and late rearing periods, thereby causing damage to the reared organisms ( Lin et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Wang

et al. 2022

Front. Cell Dev. Biol.

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High pH is one of the main stressors affecting the shrimp survival, growth, and physiology in aquaculture ponds, but the cellular and molecular mechanism responsible for high pH stress has not been elucidated in shrimp. In this study, the shrimp acid-base disturbance and gill cell alterations were significantly observed and then single cell RNA-sequencing (scRNA-seq) was performed to study the sensitive and specific responses of gill cells to high pH stress. Three main gill cell types, including pillar cells, hemocytes and septal cells were identified. By comparative scRNA-seq analysis between control and pH group, the pillar cell was regarded as the target cell type in response to high pH stress with the down-regulation of ammonia excretion and H+ transport related genes and up-regulation of immune related genes. Notedly, high pH resulted in the emergence of a new immune cell subcluster in pillar cells, with immune activation and stress defense states. Pseudotime analysis also showed that the pillar cells could transform into the functionally inhibited ion cell subclusters and functionally activated immune cell subclusters after high pH stress. Further, the regulatory network of pillar cell population was predicted by WGCNA and two transcription factors were identified. In conclusion, these results provide key insights into the shrimp gill cell-type-specific mechanisms underlying high pH stress response at a single-cell resolution.

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GsPKS24, a calcineurin B-like protein-interacting protein kinase gene from Glycine soja, positively regulates tolerance to pH stress and ABA signal transduction

Ding,

Mi,

et al. 2023

Funct Integr Genomics

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Comparative proteomic and transcriptomic analysis reveals high pH–induced expression signatures of Chinese shrimp Fenneropenaeus chinensis

Cited by 16 publications

References 68 publications

Identification of microRNAs in black tiger shrimp (Penaeus monodon) under acute low-salinity stress

Identification of microRNAs in black tiger shrimp (Penaeus monodon) under acute low-salinity stress

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

GsPKS24, a calcineurin B-like protein-interacting protein kinase gene from Glycine soja, positively regulates tolerance to pH stress and ABA signal transduction

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