Comparative proteomic analysis using 2DE‐LC‐MS/MS reveals the mechanism of Fuzhuan brick tea extract against hepatic fat accumulation in rats with nonalcoholic fatty liver disease

Abstract

Fuzhuan brick tea has received increasing attention in recent years owing to its benefits for nonalcoholic fatty liver disease (NAFLD) and associated metabolic syndrome. For exploring the ameliorative mechanism, the liver proteomes from three groups of rats fed either a normal control diet (NCD), a high fat diet (HFD), or a HFD supplemented with high-dose Fuzhuan brick tea extract (FTE) (HFD + HFTE) were comprehensively compared by quantitative proteomics using 2DE-LC-MS/MS. This is the first study of the effects of tea aqueous extract on the liver proteome of rats suffering from metabolic syndrome. The results showed that 57 proteins displayed more than 1.5-fold differences in at least one of two comparisons of HFD versus NCD and HFD versus HFD + HFTE due to HFD feeding and FTE treatment, respectively. Of them, over 75% of proteins exhibited a similar tendency of expression in the two comparisons, meaning FTE was able to correct HFD effects on rat livers. By function analyses, an extensive list of proteins was involved in sugar and lipid metabolism. Compared with HFD-fed rats, the reduced lipogenesis and enhanced β-oxidation, tricarboxylic acid cycle and respiratory chain in HFD + HFTE-fed rats, which mainly contributed to ameliorate hepatic fat accumulation and associated NAFLD. Additionally, some putative drug targets were also revealed such as COX2, PGAM1, ACACB, FAS, and ECHS1.

Kudingcha and Fuzhuan Brick Tea Prevent Obesity and Modulate Gut Microbiota in High‐Fat Diet Fed Mice

“…In recent years, a growing body of animal and human studies showed that dietary supplementation with tea was a potentially viable nutritional strategy for the prevention of obesity . It has been demonstrated that FBT and KDC consumption could prevent weight gain and metabolic disorders induced by HFD feeding . Furthermore, Lactobacillus johnsonii and another Lactobacillus sp.…”

Fuzhuan tea consumption imparts hepatoprotective effects and alters intestinal microbiota in high saturated fat diet‐fed rats

“…In high fat diet‐fed rats, Fuzhuan tea reduced body weight and hepatic steatosis, and suppressed development of hypolipidemia . Another study demonstrated that rats consuming Fuzhuan tea with HFD showed protein expression consistent with reduced lipogenesis and enhanced β‐oxidation, tricarboxylic acid cycle, and electron transport when compared with HFD‐fed rats not receiving the tea . Finally, several studies have demonstrated inhibitory effects of Fuzhuan tea on enteric pathogens and one study showed stimulation of specific commensal organisms, including Lactobacilli .…”

The hypoglycemic effect of extract/fractions from Fuzhuan Brick‐Tea in streptozotocin‐induced diabetic mice and their active components characterized by LC‐QTOF‐MS/MS

“…Recently, the attention of Fuzhuan Brick‐Tea is considerable due to its benefits to human health, especially the anti‐obesity and hypolipidemic effects (Foster et al., 2016; Li et al., 2013; Liu et al., 2015). According to the latest report, the hypoglycemic effect of water extract from Fuzhuan Brick‐Tea was investigated in type II diabetic (Huang, Liu, Huang, Yang, & Qin, 2016).…”