Comparative Proteomic Analysis Revealed the Mechanism of Tea Tree Oil Targeting Lipid Metabolism and Antioxidant System to Protect Hepatopancreatic Health in Macrobrachium rosenbergii

Abstract

Tea tree oil (TTO) is a pure natural plant essential oil. The studies evaluated the hepatopancreas lipid metabolism and antioxidant efficacy of Macrobrachium rosenbergii fed with 0 (CT group) and 100 mg/kg TTO (TT group) by label-free quantification proteomic analysis. Compared to the CT group, the TT group improved growth performance and increased the survival rate after stress. Dietary TTO also decreased hemolymph AST and ALT activities and decreased hepatopancreatic vacuolation. At the same time, hepatopancreas lipids droplets and hemolymph lipids (TG, TC, LDL-C) were decreased, and the peroxidation products content (MDA, LPO, 4-HNE) was also decreased. In addition, the levels of hepatopancreas antioxidant enzymes (T-AOC, CAT, and SOD) were increased in the TT group. With proteomic analysis, a total of 151 differentially expressed proteins (DEPs) (99 up-regulated and 52 down-regulated) were identified in the hepatopancreas. Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction analysis showed that the 16 DEPs have interactions, which are mainly involved in the pathways related to lipid metabolism (fatty acid biosynthesis, fatty acid metabolism, glycerophospholipid metabolism) and redox reaction (cytochrome P450 enzyme systems). Furthermore, the mRNA expression of 15 proteins followed the proteomic analysis with qRT-PCR validation. Pearson correlation analysis showed that fatty acids and glycerophospholipid metabolism-related proteins were highly correlated to peroxide content, glycerophospholipid metabolism, and cytochrome P450 system-related proteins (CYP1A1, GSTT1, GPX4) were highly correlated to AST and ALT. Additionally, GPX4 is closely related to peroxide content and antioxidant enzyme activity. Our results revealed that TTO plays a protective role in the hepatopancreas targeting the critical enzymes and antioxidant reactions in lipid metabolism. Provides a new perspective to elucidate the action path of TTO in protecting invertebrate hepatopancreas, highlights the influence of lipid metabolism on hepatopancreas health and the interaction between lipid metabolism and antioxidant system in the regulation of TTO.

A Sustained-Release Nanosystem with MRSA Biofilm-Dispersing and -Eradicating Abilities Accelerates Diabetic Ulcer Healing

“…Accordingly, Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein–protein interaction analyses of TTO were applied to elucidate the mechanism underlying the effects of TTO nanoliposomes (NL)/polyvinyl alcohol/chitosan (TTO-NL@PCS). 32 Following validation, the composite nano-sustained-release system reported herein was confirmed to aid in the healing of infected diabetic wounds.…”

Tea Tree Oil Mediates Antioxidant Factors Relish and Nrf2-Autophagy Axis Regulating the Lipid Metabolism of Macrobrachium rosenbergii

“…Use Two-Step SYBR ® Prime Script ® Plus RT-PCR Kit (TaKaRa, Japan) for 2 μg quantitative analysis of total RNA; the total system of sample loading was 20 μL. Based on Liu et al [ 36 ], an ABI 7500 Real-time PCR system was used for real-time quantitative PCR (RT-PCR), and the 2 −ΔΔCT method was used to calculate the relative gene expression.…”

Tea tree oil inhibits hydrogen sulfide-induced oxidative damage in chicken lungs through CYP450s/ROS pathway

“…Meanwhile, TTO termite-4-alcohol and α-pineol inhibited the production of superoxide, and then increased the levels of total antioxidant capacity (T-AOC), SOD, CAT and GSH-Px in the body, and reduced the content of malondialdehyde ( MDA ) ( Wang et al, 2021 ; Yang et al, 2022 ). TTO also regulated CYP1A1(cytochrome P450 family 1 subfamily A member 1), and GPX4 (glutathione peroxidase 4) gene expression ( Liu et al, 2022a ). In addition, further studies found that TTO treatment regulated mitochondrial apoptosis through p53-dependent pathway by regulating the expression of Bax (bcl2-associated X), Bcl-2 (B-cell lymphoma) and caspase-3 ( Ramadan et al, 2019 ).…”