Comparative Proteomic Analysis of Tolerant and Sensitive Varieties Reveals That Phenylpropanoid Biosynthesis Contributes to Salt Tolerance in Mulberry

Gan, Tiantian; Lin, Ziwei; Bao, Lijun; Hui, Tian; Cui, Xiaopeng; Huang, Yanzhen; Wang, Hexin; Su, Chao; Feng, Jiao; Zhang, Minjuan; Qian, Yonghua

doi:10.3390/ijms22179402

Cited by 26 publications

(13 citation statements)

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“…Glutathione S-transferase were induced in GZ and degraded in HN in our study. Previous research indicated that overexpression of the glutathione S-transferase could enhanced salt resistance of mulberry ( Gan et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Multiomics Analyses of Two Sorghum Cultivars Reveal the Molecular Mechanism of Salt Tolerance

et al. 2022

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Sorghum [Sorghum bicolor (L.) Moench] is one of the most important cereal crops and contains many health-promoting substances. Sorghum has high tolerance to abiotic stress and contains a variety of flavonoids compounds. Flavonoids are produced by the phenylpropanoid pathway and performed a wide range of functions in plants resistance to biotic and abiotic stress. A multiomics analysis of two sorghum cultivars (HN and GZ) under different salt treatments time (0, 24, 48, and 72) was performed. A total of 45 genes, 58 secondary metabolites, and 246 proteins were recognized with significant differential abundances in different comparison models. The common differentially expressed genes (DEGs) were allocated to the “flavonoid biosynthesis” and “phenylpropanoid biosynthesis” pathways. The most enriched pathways of the common differentially accumulating metabolites (DAMs) were “flavonoid biosynthesis,” followed by “phenylpropanoid biosynthesis” and “arginine and proline metabolism.” The common differentially expressed proteins (DEPs) were mainly distributed in “phenylpropanoid biosynthesis,” “biosynthesis of cofactors,” and “RNA transport.” Furthermore, considerable differences were observed in the accumulation of low molecular weight nonenzymatic antioxidants and the activity of antioxidant enzymes. Collectively, the results of our study support the idea that flavonoid biological pathways may play an important physiological role in the ability of sorghum to withstand salt stress.

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Section: Discussionmentioning

confidence: 99%

Multiomics Analyses of Two Sorghum Cultivars Reveal the Molecular Mechanism of Salt Tolerance

et al. 2022

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“…But those studies were mainly focused on Arabidopsis thaliana , Nicotiana tabacum , Oryza sativa , and other model plants (Dani et al 2005; Jiang et al 2007; Lakra et al 2019; Manaa et al 2011; Ndimba et al 2005; Sobhanian et al 2010; Zörb et al 2010), and some halophytic species, such as Suaeda salsa , Mesembryanthemum crystallinum , Haloxylon salicornicum , Puccinellia tenuiflora , and Porteresia coarctata (Barkla et al 2009; Li et al 2011; Panda et al 2020; Sengupta & Majumder 2009; Yu et al 2011). Proteomics approach has also been exploited to identify the key salt‐tolerance proteins by comparing the related plant species or contrasting genotypes under salinity, such as Arabidopsis thaliana/Thellungiella halophila , Triticum aestivum/Thinopyrum ponticum , Oryza sativa , Hordeum vulgare , Zea mays , and recently in Morus alba (Chen et al 2019; Damaris et al 2016; Gan et al 2021; Hussain et al 2019; Lakra et al 2018, 2019; Luo et al 2018; Pang et al 2010; Wang et al 2008; Witzel et al 2010). However, in case of millets, only a single study reported salt‐specific proteome responses, i.e.…”

Section: Discussionmentioning

confidence: 99%

Integrated physiological and comparative proteomics analysis of contrasting genotypes of pearl millet reveals underlying salt‐responsive mechanisms

Jha

Maity

Singh

et al. 2021

Physiologia Plantarum

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Salinity stress poses a significant risk to plant development and agricultural yield. Therefore, elucidation of stress‐response mechanisms has become essential to identify salt‐tolerance genes in plants. In the present study, two genotypes of pearl millet (Pennisetum glaucum L.) with contrasting tolerance for salinity exhibited differential morpho‐physiological and proteomic responses under 150 mM NaCl. The genotype IC 325825 was shown to withstand the stress better than IP 17224. The salt‐tolerance potential of IC 325825 was associated with its ability to maintain intracellular osmotic, ionic, and redox homeostasis and membrane integrity under stress. The IC 325825 genotype exhibited a higher abundance of C4 photosynthesis enzymes, efficient enzymatic and non‐enzymatic antioxidant system, and lower Na+/K+ ratio compared with IP 17224. Comparative proteomics analysis revealed greater metabolic perturbation in IP 17224 under salinity, in contrast to IC 325825 that harbored pro‐active stress‐responsive machinery, allowing its survival and better adaptability under salt stress. The differentially abundant proteins were in silico characterized for their functions, subcellular‐localization, associated pathways, and protein–protein interaction. These proteins were mainly involved in photosynthesis/response to light stimulus, carbohydrate and energy metabolism, and stress responses. Proteomics data were validated through expression profiling of the selected genes, revealing a poor correlation between protein abundance and their relative transcript levels. This study has provided novel insights into salt adaptive mechanisms in P. glaucum, demonstrating the power of proteomics‐based approaches. The critical proteins identified in the present study could be further explored as potential objects for engineering stress tolerance in salt‐sensitive major crops.

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“…In the cross-talk between different signaling networks, various salt-stress proteins regulate osmoregulation and ion transport and compartmentalize toxic ions in the vacuole. Such activities require the coordinated activity of vesicles and proteins trafficking in different cellular organelles (Nam et al, 2012;Wang et al, 2015b;Zhao et al, 2018;Gan et al, 2021). Thus, plants likely express specific proteins to perform particular functions (Figure 1).…”

Section: How Stress Proteins Enable Plants To Resist Salt Stressmentioning

confidence: 99%

Salt stress proteins in plants: An overview

et al. 2022

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Salinity stress is considered the most devastating abiotic stress for crop productivity. Accumulating different types of soluble proteins has evolved as a vital strategy that plays a central regulatory role in the growth and development of plants subjected to salt stress. In the last two decades, efforts have been undertaken to critically examine the genome structure and functions of the transcriptome in plants subjected to salinity stress. Although genomics and transcriptomics studies indicate physiological and biochemical alterations in plants, it do not reflect changes in the amount and type of proteins corresponding to gene expression at the transcriptome level. In addition, proteins are a more reliable determinant of salt tolerance than simple gene expression as they play major roles in shaping physiological traits in salt-tolerant phenotypes. However, little information is available on salt stress-responsive proteins and their possible modes of action in conferring salinity stress tolerance. In addition, a complete proteome profile under normal or stress conditions has not been established yet for any model plant species. Similarly, a complete set of low abundant and key stress regulatory proteins in plants has not been identified. Furthermore, insufficient information on post-translational modifications in salt stress regulatory proteins is available. Therefore, in recent past, studies focused on exploring changes in protein expression under salt stress, which will complement genomic, transcriptomic, and physiological studies in understanding mechanism of salt tolerance in plants. This review focused on recent studies on proteome profiling in plants subjected to salinity stress, and provide synthesis of updated literature about how salinity regulates various salt stress proteins involved in the plant salt tolerance mechanism. This review also highlights the recent reports on regulation of salt stress proteins using transgenic approaches with enhanced salt stress tolerance in crops.

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Comparative Proteomic Analysis of Tolerant and Sensitive Varieties Reveals That Phenylpropanoid Biosynthesis Contributes to Salt Tolerance in Mulberry

Cited by 26 publications

References 61 publications

Multiomics Analyses of Two Sorghum Cultivars Reveal the Molecular Mechanism of Salt Tolerance

Multiomics Analyses of Two Sorghum Cultivars Reveal the Molecular Mechanism of Salt Tolerance

Integrated physiological and comparative proteomics analysis of contrasting genotypes of pearl millet reveals underlying salt‐responsive mechanisms

Salt stress proteins in plants: An overview

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