Comparative proteomic analysis of the Arabidopsis cbl1 mutant in response to salt stress

Abstract

Many environmental stimuli, including light, biotic and abiotic stress factors, induce changes in cellular Ca2+ concentrations in plants. Such Ca2+ signatures are perceived by sensor molecules such as calcineurin B‐like (CBL) proteins. AtCBL1, a member of the CBL family which is highly inducible by multiple stress signals, is known to function in the salt stress signal transduction pathway and to positively regulate the plant tolerance to salt. To shed light into the molecular mechanisms of the salt stress response mediated by AtCBL1, a two‐dimensional DIGE proteomic approach was applied to identify the differentially expressed proteins in Arabibdopsis wild‐type and cbl1 null mutant plants in response to salt stress. Seventy‐three spots were found altered in expression by least 1.2‐fold and 50 proteins were identified by MALDI‐TOF/TOF‐MS, including some well‐known and novel salt‐responsive proteins. These proteins function in various processes, such as signal transduction, ROS scavenging, energy production, carbon fixation, metabolism, mRNA processing, protein processing and structural stability. Receptor for activated C kinase 1C (RACK1C, spot 715), a WD40 repeat protein, was up‐regulated in the cbl1 null mutant, and two rack1c mutant lines showed decreased tolerance to salt stress, suggesting that RACK1C plays a role in salt stress resistance. In conclusion, our work demonstrated the advantages of the proteomic approach in studies of plant biology and identified candidate proteins in CBL1‐mediated salt stress signaling network.

Calcium and Reactive Oxygen Species Rule the Waves of Signaling

“…Recently, a proteomic analysis of a cbl1 mutant revealed that in response to salt stress, several protein expression levels are altered compared with the wild type, further strengthening CBL1-involvement in salt stress responses (Shi et al, 2011). The affected proteins are predicted to function in various processes, such as signal transduction, ROS scavenging, energy production, carbon fixation, metabolism, mRNA and protein processing, and structural stability.…”

“…; Yang et al, 2008;Piao et al, 2010;Zhang et al, 2011Zhang et al, , 2013Chen et al, 2012;Wang et al, 2012;Deng et al, 2013;He et al, 2013). Moreover, lately, five novel CIPKs and two CBLs have been discovered in kidney bean (Phaseolus vulgaris) and 43 putative CIPK genes that are closely related to rice CIPKs have been identified in maize (Zea mays; Hamada et al, 2009;Chen et al, 2011). A function of a rice CIPK has been reported for OsCIPK31, which was found to modulate responses to abiotic stresses during seed germination and seedling growth (Piao et al, 2010), and OsCIPK23, which turned out to be a multistress-induced gene likely regulating signaling pathways during pollination and drought stress responses (Yang et al, 2008).…”

The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3

“…We used methods previously described [54], with slight modifications. The test materials were powdered in liquid nitrogen, and subsequently, the proteins were extracted using an ultrasonic crusher.…”

Leaf proteome alterations in the context of physiological and morphological responses to drought and heat stress in barley (Hordeum vulgare L.)

“…Conventional gel-based proteomics has proved useful in barley research to quantify changes in protein abundance in grains during development (Finnie et al ., 2006), in roots in response to salt stress (Witzel et al ., 2009) and in shoots in response to heat stress (Süle et al ., 2004). Proteomic research using fluorescent labels and two-dimensional difference gel electrophoresis (2D-DIGE) has been successfully applied in barley for the identification of proteins associated with malting quality (March et al ., 2012) and in Arabidopsis (Shi et al ., 2011) and wheat (Gao et al ., 2011) to identify proteins responsive to salt. By quantifying changes in protein abundance, one can gain insight into the biochemical processes that underlie the plant’s morphological and physiological acclimations to abiotic stress.…”